Quinoa seeds leach phytoecdysteroids and other compounds with anti-diabetic properties
Brittany L Graf, Alexander Poulev, Peter Kuhn, Mary H Grace, Mary Ann Lila, Ilya Raskin, Brittany L Graf, Alexander Poulev, Peter Kuhn, Mary H Grace, Mary Ann Lila, Ilya Raskin
Abstract
Quinoa (Chenopodium quinoa Willd.) contains high levels of biologically active phytoecdysteroids, which have been implicated in plant defense from insects, and have shown a range of beneficial pharmacological effects in mammals. We demonstrated that the most prevalent phytoecdysteroid, 20-hydroxyecdysone (20HE), was secreted (leached) from intact quinoa seeds into water during the initial stages of seed germination. Leaching efficiency was optimized by ethanol concentration (70% ethanol), temperature (80°C), time (4h), and solvent ratio (5 ml/g seed). When compared to extraction of macerated seeds, the leaching procedure released essentially all the 20HE available in the seeds (491 μg/g seed). The optimized quinoa leachate (QL), containing 0.86% 20HE, 1.00% total phytoecdysteroids, 2.59% flavonoid glycosides, 11.9% oil, and 20.4% protein, significantly lowered fasting blood glucose in obese, hyperglycemic mice. Leaching effectively releases and concentrates bioactive phytochemicals from quinoa seeds, providing an efficient means to produce a food-grade mixture that may be useful for anti-diabetic applications.
Keywords: Ecdysterone; Flavonoids; Metabolic syndrome; Nutraceutical; Phytoecdysteroids.
Conflict of interest statement
Conflict of interest
IR and MAL have equity in Nutrasorb LLC, which is involved in quinoa R&D.
Copyright © 2014 Elsevier Ltd. All rights reserved.
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Source: PubMed