Radioprotective Effects of Zinc and Selenium on Mice Spermatogenesis

Bagheri H, Salajegheh A, Javadi A, Amini P, Shekarchi B, Shabeeb D, Eleojo Musa A, Najafi M, Bagheri H, Salajegheh A, Javadi A, Amini P, Shekarchi B, Shabeeb D, Eleojo Musa A, Najafi M

Abstract

Background: Spermatogenesis system is one of the most radiosensitive organs in the body. A usual therapeutic dose of radiation such as the conventional 2 Gy in each fraction of radiotherapy and lower doses seen in diagnostic radiology or a radiation disaster affect the process of spermatogenesis potently. Selenium and zinc are two important elements playing key roles in the development of sperms and also have radioprotective effects.

Objective: In this study aims to evaluate the radioprotective effect of zinc and selenium against radiation-induced mice testis injury.

Material and methods: In this experimental study, 30 mice were divided equally into 6 groups, including control selenium treated, zinc treated, radiation, radiation + selenium, radiation + zinc. Treatments started from 2 days before irradiation with 2 Gy cobalt-60 gamma rays. After 37 days, all mice were killed for histopathological evaluations.

Results: Results showed that exposure to radiation caused a potent effect on spermatogenesis system. Treatment with selenium reversed these radiation effects potently, while zinc had some limited protective effects. Zinc treatment itself caused a detrimental effect on epididymis and, in combination with radiation, it leads to more damage to seminiferous tubules.

Conclusion: In contrast to previous studies that proposed zinc to protect spermatogenesis against various toxic agents, results of this study showed that although zinc may protect from some parameters, it potentiates radiation damage on seminiferous tubules and has a detrimental effect on the epididymis. By contrast, zinc and selenium could alleviate radiation-induced toxicity on the most of the evaluated parameters.

Keywords: Epididymis; Radiation; Selenium; Seminiferous Tubules; Spermatogenesis; Zinc.

Copyright: © Journal of Biomedical Physics and Engineering.

Figures

Figure 1
Figure 1
Results of radioprotective effect of selenium or zinc on mice spermatogenesis system. A: Control; B: Zinc treatment; C: Selenium treatment; D: Radiation; E: Radiation plus Zinc; F: Radiation plus Selenium. Irradiation led to severe reduction of germ cells, while zinc attenuated decreased numbers of germ cells, and selenium completely reversed radiation toxicity (H&E staining ×40).

References

    1. Marjault H B, Allemand I. Consequences of irradiation on adult spermatogenesis: Between infertility and hereditary risk. Mutat Res. 2016;770:340–8. doi: 10.1016/j.mrrev.2016.07.004.
    1. Moreno S G, Dutrillaux B, Coffigny H. High sensitivity of rat foetal germ cells to low dose-rate irradiation. Int J Radiat Biol. 2001;77:529–38. doi: 10.1080/09553000010030211.
    1. Trasler J M. Epigenetics in spermatogenesis. Mol Cell Endocrinol. 2009;306:33–6. doi: 10.1016/j.mce.2008.12.018.
    1. Coleman C N, Blakely W F, Fike J R, et al. Molecular and cellular biology of moderate-dose (1–10 Gy) radiation and potential mechanisms of radiation protection: report of a workshop at Bethesda, Maryland, December 17–18, 2001. Radiat Res. 2003;159:812–34.
    1. Holdcraft R W, Braun R E. Hormonal regulation of spermatogenesis. Int J Androl. 2004;27:335–42. doi: 10.1111/j.1365-2605.2004.00502.x.
    1. Hidiroglou M, Knipfel J E. Zinc in mammalian sperm: a review. J Dairy Sci. 1984;67:1147–56. doi: 10.3168/jds.S0022-0302(84)81416-2.
    1. Emami S, Hosseinimehr S J, Taghdisi S M, Akhlaghpoor S. Kojic acid and its manganese and zinc complexes as potential radioprotective agents. Bioorg Med Chem Lett. 2007;17:45–8. doi: 10.1016/j.bmcl.2006.09.097.
    1. Veldwijk M R, Herskind C, Sellner L, et al. Normal-tissue radioprotection by overexpression of the copper-zinc and manganese superoxide dismutase genes. Strahlenther Onkol. 2009;185:517–23. doi: 10.1007/s00066-009-1973-0.
    1. Floersheim G L, Floersheim P. Protection against ionising radiation and synergism with thiols by zinc aspartate. Br J Radiol. 1986;59:597–602. doi: 10.1259/0007-1285-59-702-597.
    1. Moslemi M K, Tavanbakhsh S. Selenium–vitamin E supplementation in infertile men: effects on semen parameters and pregnancy rate. Int J Gen Med. 2011;4:99–104. doi: 10.2147/ijgm.s16275.
    1. Amini P, Kolivand S, Saffar H, Rezapoor S, Motevaseli E, Najafi M, et al. Protective effect of Selenium-L-methionine on radiation-induced acute pneumonitis and lung fibrosis in rat. Curr Clin Pharmacol. 2018 doi: 10.2174/1574884714666181214101917.
    1. Verma P, Kunwar A, Priyadarsini K I. Effect of Low-Dose Selenium Supplementation on the Genotoxicity, Tissue Injury and Survival of Mice Exposed to Acute Whole-Body Irradiation. Biol Trace Elem Res. 2017;179:130–9. doi: 10.1007/s12011-017-0955-9.
    1. Boran C, Ozkan K U. The effect of zinc therapy on damaged testis in pre-pubertal rats. Pediatr Surg Int. 2004;20:444–8. doi: 10.1007/s00383-004-1173-z.
    1. Ayhanci A, Yaman S, Appak S, Gunes S. Hematoprotective effect of seleno-L-methionine on cyclophosphamide toxicity in rats. Drug Chem Toxicol. 2009;32:424–8. doi: 10.1080/01480540903130682.
    1. Khan S, Adhikari J S, Rizvi M A, Chaudhury N K. Radioprotective potential of melatonin against 60 Co γ-ray-induced testicular injury in male C57BL/6 mice. J Biomed Sci. 2015;22:61. doi: 10.1186/s12929-015-0156-9.
    1. Mahdavi M, Mozdarani H. Protective effects of famotidine and vitamin C against radiation induced cellular damage in mouse spermatogenesis process. International Journal of Radiation Research. 2011;8:223.
    1. Songthaveesin C, Saikhun J, Kitiyanant Y, Pavasuthipaisit K. Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study. Asian Journal of Andrology. 2004;6:331–6.
    1. Shaban N Z, Zahran A M A, El-Rashidy F H, Kodous A S A. Protective role of hesperidin against γ-radiation-induced oxidative stress and apoptosis in rat testis. Journal of Biological Research-Thessaloniki. 2017;24:5.
    1. Sieber F, Muir S A, Cohen E P, Fish B L, et al. Dietary selenium for the mitigation of radiation injury: effects of selenium dose escalation and timing of supplementation. Radiat Res. 2011;176:366–74. [ ]
    1. Sieber F, Muir S A, Cohen E P, North P E, et al. High-dose selenium for the mitigation of radiation injury: a pilot study in a rat model. Radiat Res. 2009;171:368–73. doi: 10.1667/0033-7587-171.3.368.
    1. Puspitasari I M, Abdulah R, Yamazaki C, Kameo S, Nakano T, Koyama H. Updates on clinical studies of selenium supplementation in radiotherapy. Radiat Oncol. 2014;9:125. doi: 10.1186/1748-717X-9-125. [ ]
    1. Dani V, Dhawan D K. Radioprotective role of zinc following single dose radioiodine (131I) exposure to red blood cells of rats. Indian J Med Res. 2005;122:338–42.

Source: PubMed

Sponsorit ja yhteistyökumppanit

Sairaudet

Huumeiden interventiot

3
Tilaa