A six-month randomized controlled trial of whole soy and isoflavones daidzein on body composition in equol-producing postmenopausal women with prehypertension

Zhao-Min Liu, Suzanne C Ho, Yu-Ming Chen, Jean Woo, Zhao-Min Liu, Suzanne C Ho, Yu-Ming Chen, Jean Woo

Abstract

Objectives: This paper reported the effects of commonly used whole soy foods (soy flour) and purified daidzein (one of the major isoflavones and the precursor of equol) on changes in anthropometric measurements and body composition in a 6-month double-blind, randomized, placebo-controlled trial among prehypertensive postmenopausal women who are also equol producers.

Methods: 270 eligible women were randomized to either one of the three treatments: 40 g soy flour (whole soy group), 40 g low-fat milk powder + 63 mg daidzein (daidzein group), or 40 g low-fat milk powder (placebo group) daily each for 6 months. Anthropometric indicators and body composition were measured before and after intervention.

Results: 253 subjects completed the study with good compliance. Urinary isoflavones levels suggested good compliance of subjects with supplementation. Whole soy and purified daidzein had no significant effect on body weight, body mass index (BMI), waist and hip circumferences, waist to hip ratio (WHR), body fat percentage, fat mass, and free fat mass.

Conclusion: Six-month consumption of whole soy and purified daidzein at provided dosage had no improvement on body weight and composition compared with isocaloric milk placebo among prehypertensive equol-producing postmenopausal women. This trial is registered with ClinicalTrials.gov NCT01270737.

Figures

Figure 1
Figure 1
GI discomfort: gastrointestinal discomfort; BP: blood pressure; Whole soy group: intake 40 g soy flour per day; Daidzein group: intake 63 mg daidzein + 40 g low-fat milk powder per day; Placebo group: intake 40 g low-fat milk powder per day.

References

    1. Kelly T, Yang W, Chen CS, Reynolds K, He J. Global burden of obesity in 2005 and projections to 2030. International Journal of Obesity. 2008;32(9):1431–1437.
    1. Elmadfa I, Meyer AL. Body composition, changing physiological functions and nutrient requirements of the elderly. Annals of Nutrition and Metabolism. 2008;52(supplement 1):2–5.
    1. Misso ML, Jang C, Adams J, et al. Differential expression of factors involved in fat metabolism with age and the menopause transition. Maturitas. 2005;51(3):299–306.
    1. Sipilä S. Body composition and muscle performance during menopause and hormone replacement therapy. Journal of Endocrinological Investigation. 2003;26(9):893–901.
    1. Vaughn N, Rizzo A, Doane D, Beverly JL, de Mejia EG. Intracerebroventricular administration of soy protein hydrolysates reduces body weight without affecting food intake in rats. Plant Foods for Human Nutrition. 2008;63(1):41–46.
    1. Cederroth CR, Vinciguerra M, Gjinovci A, et al. Dietary phytoestrogens activate AMP-activated protein kinase with improvement in lipid and glucose metabolism. Diabetes. 2008;57(5):1176–1185.
    1. Velasquez MT, Bhathena SJ. Role of dietary soy protein in obesity. International Journal of Medical Sciences. 2007;4(2):72–82.
    1. Aoyama T, Fukui K, Takamatsu K, Hashimoto Y, Yamamoto T. Soy protein isolate and its hydrolysate reduce body fat of dietary obese rats and genetically obese mice (yellow KK) Nutrition. 2000;16(5):349–354.
    1. Naaz A, Yellayi S, Zakroczymski MA, et al. The soy isoflavone genistein decreases adipose deposition in mice. Endocrinology. 2003;144(8):3315–3320.
    1. Guthrie JR, Ball M, Murkies A, Dennerstein L. Dietary phytoestrogen intake in mid-life Australian-born women: relationship to health variables. Climacteric. 2000;3(4):254–261.
    1. Yamori Y. Worldwide epidemic of obesity: hope for Japanese diets. Clinical and Experimental Pharmacology & Physiology. 2004;31(supplement 2):S2–S4.
    1. Goodman-Gruen D, Kritz-Silverstein D. Usual dietary isoflavone intake and body composition in postmenopausal women. Menopause. 2003;10(5):427–432.
    1. Nachtigal MC, Patterson RE, Stratton KL, Adams LA, Shattuck AL, White E. Dietary supplements and weight control in a middle-age population. Journal of Alternative and Complementary Medicine. 2005;11(5):909–915.
    1. Choquette S, Riesco E, Cormier E, Dion T, Aubertin-Leheudre M, Dionne IJ. Effects of soya isoflavones and exercise on body composition and clinical risk factors of cardiovascular diseases in overweight postmenopausal women: a 6-month double-blind controlled trial. The British Journal of Nutrition. 2011;105(8):1199–1209.
    1. Christie DR, Grant J, Darnell BE, Chapman VR, Gastaldelli A, Sites CK. Metabolic effects of soy supplementation in postmenopausal Caucasian and African American women: a randomized, placebo-controlled trial. The American Journal of Obstetrics and Gynecology. 2010;203(2):153.e1–153.e9.
    1. Sites CK, Cooper BC, Toth MJ, Gastaldelli A, Arabshahi A, Barnes S. Effect of a daily supplement of soy protein on body composition and insulin secretion in postmenopausal women. Fertility and Sterility. 2007;88(6):1609–1617.
    1. Liao F, Shieh M, Yang S, Lin S, Chien Y. Effectiveness of a soy-based compared with a traditional low-calorie diet on weight loss and lipid levels in overweight adults. Nutrition. 2007;23(7-8):551–556.
    1. Azadbakht L, Nurbakhsh S. Effect of soy drink replacement in a weight reducing diet on anthropometric values and blood pressure among overweight and obese female youths. Asia Pacific Journal of Clinical Nutrition. 2011;20(3):383–389.
    1. Takahira M, Noda K, Fukushima M, et al. Randomized, double-blind, controlled, comparative trial of formula food containing soy protein versus milk protein in visceral fat obesity: FLAVO study. Circulation Journal. 2011;75(9):2235–2243.
    1. Baer DJ, Stote KS, Paul DR, Harris GK, Rumpler WV, Clevidence BA. Whey protein but not soy protein supplementation alters body weight and composition in free-living overweight and obese adults. Journal of Nutrition. 2011;141(8):1489–1494.
    1. Anderson JW, Fuller J, Patterson K, Blair R, Tabor A. Soy compared to casein meal replacement shakes with energy-restricted diets for obese women: randomized controlled trial. Metabolism: Clinical and Experimental. 2007;56(2):280–288.
    1. Wu J, Oka J, Ezaki J, et al. Possible role of equol status in the effects of isoflavone on bone and fat mass in postmenopausal Japanese women: a double-blind, randomized, controlled trial. Menopause. 2007;14(5):866–874.
    1. Muthyala RS, Ju YH, Sheng S, et al. Equol, a natural estrogenic metabolite from soy isoflavones: convenient preparation and resolution of R- and S-equols and their differing binding and biological activity through estrogen receptors alpha and beta. Bioorganic and Medicinal Chemistry. 2004;12(6):1559–1567.
    1. Setchell KDR, Brown NM, Lydeking-Olsen E. The clinical importance of the metabolite equol—a clue to the effectiveness of soy and its isoflavones. Journal of Nutrition. 2002;132(12):3577–3584.
    1. Welty FK, Lee KS, Lew NS, Zhou J. Effect of soy nuts on blood pressure and lipid levels in hypertensive, prehypertensive, and normotensive postmenopausal women. Archives of Internal Medicine. 2007;167(10):1060–1067.
    1. Kreijkamp-Kaspers S, Kok L, Bots ML, Grobbee DE, Lampe JW, van der Schouw YT. Randomized controlled trial of the effects of soy protein containing isoflavones on vascular function in postmenopausal women. The American Journal of Clinical Nutrition. 2005;81(1):189–195.
    1. Hooper L, Kroon PA, Rimm EB, et al. Flavonoids, flavonoid-rich foods, and cardiovascular risk: a meta-analysis of randomized controlled trials. The American Journal of Clinical Nutrition. 2008;88(1):38–50.
    1. Messina M, Lampe JW, Birt DF, et al. Reductionism and the narrowing nutrition perspective: time for reevaluation and emphasis on food synergy. Journal of the American Dietetic Association. 2001;101(12):1416–1419.
    1. Setchell KDR, Cole SJ. Method of defining equol-producer status and its frequency among vegetarians. Journal of Nutrition. 2006;136(8):2188–2193.
    1. Yue-Xin Y. China Food Composition 2002. Beijing, China: Institute of Nutrition and Food Safety, Peking University Medical Press; 2002.
    1. Lukaski HC, Johnson PE, Bolonchuk WW, Lykken GI. Assessment of fat-free mass using bioelectrical impedance measurements of the human body. The American Journal of Clinical Nutrition. 1985;41(4):810–817.
    1. Song T, Barua K, Buseman G, Murphy PA. Soy isoflavone analysis: quality control and a new internal standard. The American Journal of Clinical Nutrition. 1998;68(6, supplement):1474S–1479S.
    1. St-Onge M, Claps N, Wolper C, Heymsfield SB. Supplementation with soy-protein-rich foods does not enhance weight loss. Journal of the American Dietetic Association. 2007;107(3):500–505.
    1. Kok L, Kreijkamp-Kaspers S, Grobbee DE, Lampe JW, van der Schouw YT. Soy isoflavones, body composition, and physical performance. Maturitas. 2005;52(2):102–110.
    1. Yamashita T, Sasahara T, Pomeroy SE, Collier G, Nestel PJ. Arterial compliance, blood pressure, plasma leptin, and plasma lipids in women are improved with weight reduction equally with a meat-based diet and a plant-based diet. Metabolism: Clinical and Experimental. 1998;47(11):1308–1314.
    1. Lang V, Bellisle F, Oppert J, et al. Satiating effect of proteins in healthy subjects: a comparison of egg albumin, casein, gelatin, soy protein, pea protein, and wheat gluten. The American Journal of Clinical Nutrition. 1998;67(6):1197–1204.
    1. Lang V, Bellisle F, Alamowitch C, et al. Varying the protein source in mixed meal modifies glucose, insulin and glucagon kinetics in healthy men, has weak effects on subjective satiety and fails to affect food intake. European Journal of Clinical Nutrition. 1999;53(12):959–965.
    1. Yalçin AS. Emerging therapeutic potential of whey proteins and peptides. Current Pharmaceutical Design. 2006;12(13):1637–1643.
    1. Maesta N, Nahas EAP, Nahas-Neto J, et al. Effects of soy protein and resistance exercise on body composition and blood lipids in postmenopausal women. Maturitas. 2007;56(4):350–358.
    1. Moeller LE, Peterson CT, Hanson KB, et al. Isoflavone-rich soy protein prevents loss of hip lean mass but does not prevent the shift in regional fat distribution in perimenopausal women. Menopause. 2003;10(4):322–331.
    1. Allison DB, Gadbury G, Schwartz LG, et al. A novel soy-based meal replacement formula for weight loss among obese individuals: a randomized controlled clinical trial. European Journal of Clinical Nutrition. 2003;57(4):514–522.
    1. Liu ZM, Ho SC, Chen YM, Ho YP. A mild favorable effect of soy protein with isoflavones on body compositiona 6-month double-blind randomized placebo-controlled trial among Chinese postmenopausal women. International Journal of Obesity. 2010;34(2):309–318.
    1. Messina M, Messina V. The role of soy in vegetarian diets. Nutrients. 2010;2(8):855–888.
    1. Society CN. Dietary Guidelines for Chinese Residents 2007. 2007,
    1. Clarkson TB, Anthony MS. Phytoestrogens and coronary heart disease. Bailliere’s Clinical Endocrinology and Metabolism. 1998;12(4):589–604.
    1. Prentice AM, Jebb SA. Beyond body mass index. Obesity Reviews. 2001;2(3):141–147.
    1. Houtkooper LB, Lohman TG, Going SB, Howell WH. Why bioelectrical impedance analysis should be used for estimating adiposity. The American Journal of Clinical Nutrition. 1996;64(3, supplement):436S–448S.
    1. Heitmann BL. Impedance: a valid method in assessment of body composition? European Journal of Clinical Nutrition. 1994;48(4):228–240.
    1. Kushner RF. Bioelectrical impedance analysis: a review of principles and applications. Journal of the American College of Nutrition. 1992;11(2):199–209.
    1. Ellis KJ. Human body composition: in vivo methods. Physiological Reviews. 2000;80(2):649–680.
    1. Després JP. Intra-abdominal obesity: an untreated risk factor for type 2 diabetes and cardiovascular disease. Journal of Endocrinological Investigation. 2006;29(3, supplement):77–82.

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