Polyphenols and Overfeeding (Poly-Nut)
Polyphenols: Protection From Overfeeding-induced Insulin Resistance?
Obesity is a complex and multifactorial disease representing a major public health challenge. Indeed, overweight and obese people carry a major risk of developing insulin resistance and type 2 diabetes mellitus. It has been demonstrated that polyphenols could be used to counteract some of the mechanisms involved in the generation of insulin resistance. The model of overfeeding represents a very good study model of the metabolic complications of obesity, since it has been shown to induce a transient state of insulin resistance in the human.
The purpose of this research is to study the effects of a overfeeding (+50% of daily caloric needs over 31 days) with or without polyphenols supplementation (2g/days over 31 days) on insulin sensitivity measured in the setting of a hyperinsulinemic euglycemic clamp and on post prandial partitioning of exogenous lipids after a test meal.
During 31 days, the volunteers will consume chocolate bars, chips, chocolate breads and cola in addition to their usual diet and will be supplemented with 2g of grape polyphenols extract or with placebo.
調査の概要
状態
条件
研究の種類
入学 (実際)
段階
- 適用できない
連絡先と場所
研究場所
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Pierre-bénite、フランス、69495
- Rhône Alpes Human Nutrition Center (CRNH Rhône-Alpes)
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参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- Male
- Aged from 18 to 55 years
- Body Mass Index of 23 to 27 kg/m²
- Normal blood pressure
- Fasting glycemia < 7 mmol/L
- CRPus < 10mg/L
- Triglycerides ≤ 3mmol/L
Exclusion Criteria:
- Medical or surgical history which may affect the results (renal -cardiovascular - hepatic- endocrine-inflammatory diseases)
- Subjects under treatment which may interfere with the measured parameters
- Eating disorder
- Intensive sportive activity
- Dairy products allergy or intolerance
研究計画
研究はどのように設計されていますか？
デザインの詳細
- 主な目的：基礎科学
- 割り当て：ランダム化
- 介入モデル：並列代入
- マスキング：4倍
武器と介入
参加者グループ / アーム |
介入・治療 |
|---|---|
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実験的：2g of grape polyphenol extract supplement
Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
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Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
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プラセボコンパレーター：2g of placebo (lactose)
Men will have to consume daily 2g of placebo during the 31 days of overfeeding.
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Men will have to consume daily 2g of placebo during the 31 days of overfeeding.
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この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Peripheral insulin sensitivity
時間枠：During 31 days
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Volunteers will have peripheral and hepatic insulin sensitivity determined by using a two-stage hyperinsulinemic euglycemic clamp with stable isotopically labeled tracer infusion.
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During 31 days
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二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Hepatic insulin sensitivity
時間枠：before and after the 31 days of overfeeding
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Endogenous glucose production (EGP) will be determined during the last 30 minutes of the low-dose insulin infusion.
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before and after the 31 days of overfeeding
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Change in genes expression in adipose and muscle tissues
時間枠：Before and after the 31 days of overfeeding
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RT-PCR
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Before and after the 31 days of overfeeding
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Anthropometry, Fat quantification and Abdominal fat distribution
時間枠：During 31 days
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Weight, height, waist and hip circumferences, fat mass using dual x-ray absorptiometry (DEXA) and abdominal fat distribution using resonance magnetic imaging (RMI).
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During 31 days
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Fasting and Postprandial partitioning of exogenous lipid
時間枠：Fasting and during all the postprandial period (0-300 minutes)
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13C enrichment will be measured in blood samples and breath test during a test meal performed before and after the 31 days of overfeeding.
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Fasting and during all the postprandial period (0-300 minutes)
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Modification in intestinal microbiota
時間枠：Before and after the 31 days of overfeeding
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Feces analyses
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Before and after the 31 days of overfeeding
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Energy expenditure and substrate oxidation with indirect calorimetry
時間枠：Fasting and during all the postprandial period (0-300 minutes)
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Energy metabolism will be measured before and after the 31 days of overfeeding.
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Fasting and during all the postprandial period (0-300 minutes)
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協力者と研究者
スポンサー
捜査官
- 主任研究者：Martine LAVILLE, MD, PhD、Rhône Alpes Human Nutrition Center (CRNH Rhône-Alpes)
出版物と役立つリンク
一般刊行物
- Bartova S, Madrid-Gambin F, Fernandez L, Carayol J, Meugnier E, Segrestin B, Delage P, Vionnet N, Boizot A, Laville M, Vidal H, Marco S, Hager J, Moco S. Grape polyphenols decrease circulating branched chain amino acids in overfed adults. Front Nutr. 2022 Oct 26;9:998044. doi: 10.3389/fnut.2022.998044. eCollection 2022.
- Segrestin B, Delage P, Nemeth A, Seyssel K, Disse E, Nazare JA, Lambert-Porcheron S, Meiller L, Sauvinet V, Chanon S, Simon C, Ratiney H, Beuf O, Pralong F, Yassin NA, Boizot A, Gachet M, Burton-Pimentel KJ, Vidal H, Meugnier E, Vionnet N, Laville M. Polyphenol Supplementation Did Not Affect Insulin Sensitivity and Fat Deposition During One-Month Overfeeding in Randomized Placebo-Controlled Trials in Men and in Women. Front Nutr. 2022 May 9;9:854255. doi: 10.3389/fnut.2022.854255. eCollection 2022.
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
本研究に関する用語
キーワード
その他の研究ID番号
- 2013-807
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