Polyphenols and Overfeeding (Poly-Nut)
Polyphenols: Protection From Overfeeding-induced Insulin Resistance?
Obesity is a complex and multifactorial disease representing a major public health challenge. Indeed, overweight and obese people carry a major risk of developing insulin resistance and type 2 diabetes mellitus. It has been demonstrated that polyphenols could be used to counteract some of the mechanisms involved in the generation of insulin resistance. The model of overfeeding represents a very good study model of the metabolic complications of obesity, since it has been shown to induce a transient state of insulin resistance in the human.
The purpose of this research is to study the effects of a overfeeding (+50% of daily caloric needs over 31 days) with or without polyphenols supplementation (2g/days over 31 days) on insulin sensitivity measured in the setting of a hyperinsulinemic euglycemic clamp and on post prandial partitioning of exogenous lipids after a test meal.
During 31 days, the volunteers will consume chocolate bars, chips, chocolate breads and cola in addition to their usual diet and will be supplemented with 2g of grape polyphenols extract or with placebo.
研究概览
地位
条件
研究类型
注册 (实际的)
阶段
- 不适用
联系人和位置
学习地点
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Pierre-bénite、法国、69495
- Rhône Alpes Human Nutrition Center (CRNH Rhône-Alpes)
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- Male
- Aged from 18 to 55 years
- Body Mass Index of 23 to 27 kg/m²
- Normal blood pressure
- Fasting glycemia < 7 mmol/L
- CRPus < 10mg/L
- Triglycerides ≤ 3mmol/L
Exclusion Criteria:
- Medical or surgical history which may affect the results (renal -cardiovascular - hepatic- endocrine-inflammatory diseases)
- Subjects under treatment which may interfere with the measured parameters
- Eating disorder
- Intensive sportive activity
- Dairy products allergy or intolerance
学习计划
研究是如何设计的？
设计细节
- 主要用途：基础科学
- 分配：随机化
- 介入模型：并行分配
- 屏蔽：四人间
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
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实验性的：2g of grape polyphenol extract supplement
Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
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Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
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安慰剂比较：2g of placebo (lactose)
Men will have to consume daily 2g of placebo during the 31 days of overfeeding.
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Men will have to consume daily 2g of placebo during the 31 days of overfeeding.
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Peripheral insulin sensitivity
大体时间：During 31 days
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Volunteers will have peripheral and hepatic insulin sensitivity determined by using a two-stage hyperinsulinemic euglycemic clamp with stable isotopically labeled tracer infusion.
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During 31 days
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Hepatic insulin sensitivity
大体时间：before and after the 31 days of overfeeding
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Endogenous glucose production (EGP) will be determined during the last 30 minutes of the low-dose insulin infusion.
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before and after the 31 days of overfeeding
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Change in genes expression in adipose and muscle tissues
大体时间：Before and after the 31 days of overfeeding
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RT-PCR
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Before and after the 31 days of overfeeding
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Anthropometry, Fat quantification and Abdominal fat distribution
大体时间：During 31 days
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Weight, height, waist and hip circumferences, fat mass using dual x-ray absorptiometry (DEXA) and abdominal fat distribution using resonance magnetic imaging (RMI).
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During 31 days
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Fasting and Postprandial partitioning of exogenous lipid
大体时间：Fasting and during all the postprandial period (0-300 minutes)
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13C enrichment will be measured in blood samples and breath test during a test meal performed before and after the 31 days of overfeeding.
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Fasting and during all the postprandial period (0-300 minutes)
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Modification in intestinal microbiota
大体时间：Before and after the 31 days of overfeeding
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Feces analyses
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Before and after the 31 days of overfeeding
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Energy expenditure and substrate oxidation with indirect calorimetry
大体时间：Fasting and during all the postprandial period (0-300 minutes)
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Energy metabolism will be measured before and after the 31 days of overfeeding.
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Fasting and during all the postprandial period (0-300 minutes)
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合作者和调查者
调查人员
- 首席研究员：Martine LAVILLE, MD, PhD、Rhône Alpes Human Nutrition Center (CRNH Rhône-Alpes)
出版物和有用的链接
一般刊物
- Bartova S, Madrid-Gambin F, Fernandez L, Carayol J, Meugnier E, Segrestin B, Delage P, Vionnet N, Boizot A, Laville M, Vidal H, Marco S, Hager J, Moco S. Grape polyphenols decrease circulating branched chain amino acids in overfed adults. Front Nutr. 2022 Oct 26;9:998044. doi: 10.3389/fnut.2022.998044. eCollection 2022.
- Segrestin B, Delage P, Nemeth A, Seyssel K, Disse E, Nazare JA, Lambert-Porcheron S, Meiller L, Sauvinet V, Chanon S, Simon C, Ratiney H, Beuf O, Pralong F, Yassin NA, Boizot A, Gachet M, Burton-Pimentel KJ, Vidal H, Meugnier E, Vionnet N, Laville M. Polyphenol Supplementation Did Not Affect Insulin Sensitivity and Fat Deposition During One-Month Overfeeding in Randomized Placebo-Controlled Trials in Men and in Women. Front Nutr. 2022 May 9;9:854255. doi: 10.3389/fnut.2022.854255. eCollection 2022.
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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