Effects of Chlorhexidine on Human Gingival Fibroblasts
In Vitro Effects of a New Chlorhexidine-based Solution on Human Gingival Fibroblasts Survival and Function
This prospective in vitro study aims to evaluate the effects of two chlorhexidine-based mouthwash formulations on the survival and function of human gingival fibroblasts (hGFs). Gingival fibroblasts obtained from discarded gingival tissue collected during routine dental procedures will be cultured and exposed to different concentrations of a conventional 0.2% chlorhexidine mouthwash and a 0.2% chlorhexidine mouthwash supplemented with N-acetylcysteine (NAC) and hyaluronic acid (HA).
Cell viability, apoptosis, migration, and gene expression will be assessed at multiple time points using Trypan Blue exclusion assay, fluorescence-activated cell sorting (FACS), scratch assay, and quantitative real-time PCR. The study is designed to investigate the concentration-dependent effects of chlorhexidine on fibroblast viability and wound-healing-related functions and to determine whether the addition of NAC and HA may improve the biological compatibility of chlorhexidine-based formulations.
Studieoversikt
Status
Studietype
Registrering (Antatt)
Kontakter og plasseringer
Studiekontakt
- Navn: Roberto Professor Roberto Rotundo, Associate Professor
- Telefonnummer: 0226432643
- E-post: rotundo.roberto@hsr.it
Deltakelseskriterier
Kvalifikasjonskriterier
Alder som er kvalifisert for studier
- Voksen
- Eldre voksen
Tar imot friske frivillige
Prøvetakingsmetode
Studiepopulasjon
Beskrivelse
Inclusion Criteria:
- Male or female adults aged 18 years or older.
- Undergoing routine dental surgical procedures (e.g., tooth extraction or gingivectomy).
- Willing and able to provide written informed consent for the use of discarded gingival tissue for research purposes.
- Systemically healthy.
Exclusion Criteria:
- Presence of systemic diseases.
- Presence of relevant medical comorbidities.
- Current smoker.
- Refusal to provide informed consent for the use of discarded biological material.
Studieplan
Hvordan er studiet utformet?
Designdetaljer
Kohorter og intervensjoner
Gruppe / Kohort |
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Healthy Adult Donors
Human Gingival Fibroblast Cultures
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Hva måler studien?
Primære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
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Human Gingival Fibroblast Viability
Tidsramme: 24 hours, 3 days and 7 days from the start of cell culture exposure
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Cell viability of human gingival fibroblast cultures exposed in vitro to different concentrations of chlorhexidine-based mouthwash formulations will be assessed using Trypan Blue exclusion assay and live/dead analysis by fluorescence-activated cell sorting (FACS).
Viability will be expressed as the proportion of viable cells compared with total counted cells.
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24 hours, 3 days and 7 days from the start of cell culture exposure
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Samarbeidspartnere og etterforskere
Sponsor
Studierekorddatoer
Studer hoveddatoer
Studiestart (Antatt)
Primær fullføring (Antatt)
Studiet fullført (Antatt)
Datoer for studieregistrering
Først innsendt
Først innsendt som oppfylte QC-kriteriene
Først lagt ut (Faktiske)
Oppdateringer av studieposter
Sist oppdatering lagt ut (Faktiske)
Siste oppdatering sendt inn som oppfylte QC-kriteriene
Sist bekreftet
Mer informasjon
Begreper knyttet til denne studien
Andre studie-ID-numre
- Chx.01
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