Characterization of Airway Mucus in Bronchiectasis Patients and Healthy Controls
研究概览
地位
条件
详细说明
In this study the investigators will investigate the mucus properties in individuals with idiopathic bronchiectasis and healthy individuals without a history of lung disease. In order to understand how mucus is abnormal in disease conditions, the investigators need to understand the mucus biochemical and biophysical properties in healthy individuals. In this study the investigators will prospectively recruit patients with bronchiectasis and healthy individuals from the community to provide airway sputum samples. In these samples the investigators will measure an array of mucus properties. This will help our understanding of the mucus-obstructive lung diseases and facilitate the development of appropriate and effective disease prevention strategies.
A key question is whether mucus is hyper-concentrated and has abnormal biophysical properties in individuals with mucus-obstructive lung disease. To answer this question, the investigators need to understand the properties of the mucus in individuals with idiopathic bronchiectasis and no history of lung disease. This study will allow us to collect airway mucus (sputum) from individuals with bronchiectasis and healthy individuals with no history of lung disease.
研究类型
注册 (实际的)
联系人和位置
学习地点
-
-
North Carolina
-
Chapel Hill、North Carolina、美国、27599
- Center for Environmental Medicine Asthma and Lung Biology
-
Chapel Hill、North Carolina、美国、27599
- Marsico Research Institure at Meadowmont
-
-
参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Participants who meet all the following criteria are eligible for the study:
- Written informed consent
- Bronchiectasis patients: Available CT of the chest that shows evidence of dilated airways fulfilling radiographic criteria for bronchiectasis in more than one lobe and chronic cough.
Participants who meet any of the following criteria will not be eligible for the study:
- Healthy individuals: A history of lung disease, current asthma symptoms or medication use, premature birth (<37 week gestation), a history of lung disease of infancy, or neurological or cardiovascular illness, current smoker or a history of smoking tobacco products.
- Bronchiectasis patients: Premature birth (<37 week gestation), a history of lung disease of infancy, or neurological or cardiovascular illness.
- Younger than 18 years of age
学习计划
研究是如何设计的？
设计细节
队列和干预
团体/队列 |
|---|
|
control
healthy individuals with no history of lung disease
|
|
bronchiectasis patients
individuals with a diagnoses of bronchiectasis
|
研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Mucus concentration (% solids)
大体时间：6 months
|
The percentage of solid content of sputum (an index of hydration) will be calculated my measuring the wet to dry weight ratio using a microbalance (mean percent solids ± standard deviation).
|
6 months
|
次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Biophysical properties of mucus by rheology
大体时间：6 months
|
The rheological properties of the sputum will be assayed by macro-rheology (cone and plate) and microbead rheology.
Macro-rheology provides measures of the viscosity (median log viscosity in Pascal (range)) and elasticity (median log elasticity in Pascal (range) of the sputum.
Microbead rheology is performed by analyzing the diffusive motion of embedded 1μm tracer particles (6).
The primary outcome measure from microbead rheology will be the mean square displacement MSD(Mean squared displacement) (median MSD cm2 s-1 (range)).
|
6 months
|
|
Mucin composition by mass spectroscopy
大体时间：6 months
|
We will use mass spectroscopy to determine the relative abundance of the two major polymeric mucin proteins, MUC5B(Mucin type 5B)and MUC5AC(Mucin type 5AC), which are responsible for the viscoelastic properties of mucus.
|
6 months
|
|
Sputum extracellular nucleotide analysis
大体时间：6 months
|
Sputum adenosine and adenine nucleotides will be ethenoderivatized and measured by HPLC(High performance liquid chromatography) analysis (7) (median nucleotide concentration in μg/ml (range)).
|
6 months
|
|
Osmotic Pressure
大体时间：6 months
|
The osmotic pressure of the mucus will be assessed by placing an aliquot of mucus onto an osmometer with a 10kDa (10 kilodaltons) molecular weight polyethersulfone membrane (Millipore) separating the test chamber and reference chamber filled with PBS (phosphate-buffered saline) (mean osmotic pressure in Pascal ± standard deviation).
|
6 months
|
|
Total mucin concentration
大体时间：6 months
|
Mucins will be quantitated using sepharose CL2B(Cross-linked derivative of Sepharose 2B) UPLC(Ultra performance liquid chromatography) coupled to an Optilab interference refractometer with a filter at 680 nm and parallel flow chambers for solvent and the solution (mean mucin concentration (μg/ml) ± standard deviation) (4).
With this approach, we will ensure that the solute is at full Donnan equilibrium with the solution within the context of gel chromatography, which creates an accurate equilibration of solute and solvent.
This procedure also separates the mucins from contaminating proteins, allowing the mucins to be isolated for biochemical studies.
DNAse can be added if significant DNA is present.
|
6 months
|
|
polymeric DNA concentration
大体时间：6 months
|
The quantification of free DNA will be performed using the DNA Quant-iT PicoGreen assay (Molecular Probes, Inc., Eugene, OR, USA), which utilizes an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (median log DNA concentration (μg/ml) (range)).
|
6 months
|
合作者和调查者
调查人员
- 学习椅：Richard Boucher, MD、University of North Carolina, Chapel Hill
- 首席研究员：Kathryn Ramsey、University of North Carolina, Chapel Hill
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
此信息直接从 clinicaltrials.gov 网站检索，没有任何更改。如果您有任何更改、删除或更新研究详细信息的请求，请联系 register@clinicaltrials.gov. clinicaltrials.gov 上实施更改，我们的网站上也会自动更新.