Gingival TrxR1 Expression in Periodontal Health and Periodontitis
2026年5月14日 更新者：Fuat Cetinkaya、Ataturk University
Thioredoxin Reductase 1 Levels in Gingival Tissue as an Oxidative Stress Biomarker in Periodontitis
This study aims to investigate the role of the thioredoxin system in the development and progression of periodontitis.
Researchers will examine the levels of specific antioxidant markers, such as thioredoxin reductase 1 (TrxR1), glutathione (GSH), and malondialdehyde (MDA), in gingival tissue samples collected from healthy individuals and patients with different stages of periodontal disease.
The goal is to determine if TrxR1 levels can serve as a potential biomarker for diagnosing the severity and activity of periodontitis.
研究概览
研究类型
观察性的
注册 (实际的)
60
联系人和位置
本节提供了进行研究的人员的详细联系信息，以及有关进行该研究的地点的信息。
学习地点
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Erzurum、土耳其（türkiye）、25240
- Ataturk University Faculty of Dentistry
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参与标准
研究人员寻找符合特定描述的人，称为资格标准。这些标准的一些例子是一个人的一般健康状况或先前的治疗。
资格标准
适合学习的年龄
- 成人
- 年长者
接受健康志愿者
是的
取样方法
非概率样本
研究人群
Patients visiting the Department of Periodontology at Atatürk University Faculty of Dentistry who meet the clinical criteria for health or periodontitis.
描述
Inclusion Criteria:
- Being between the ages of 18 and 70.
- Having no known systemic disease.
- Non-smokers.
- No history of medication or supplement use in the last 3 months.
- For the Periodontitis groups: Interdental clinical attachment loss (CAL), probing pocket depth (PPD), and radiographic bone loss according to 2017 World Workshop criteria.
Exclusion Criteria:
- Smoking.
- Pregnancy or breastfeeding.
- Presence of a systemic disease (e.g., diabetes mellitus, hypertension, rheumatoid arthritis, cancer, thyroid disorders, kidney or lung diseases).
- History of any periodontal surgical procedure within the last 6 months.
- Use of medication or supplements within the last 3 months.
学习计划
本节提供研究计划的详细信息，包括研究的设计方式和研究的衡量标准。
研究是如何设计的？
设计细节
队列和干预
团体/队列 |
干预/治疗 |
|---|---|
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Healthy Control Group
Systemically and periodontally healthy individuals with no history of bone loss, probing depths ≤ 3 mm, and bleeding on probing < 10%.
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Gingival tissue samples are collected from all participants.
These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods.
Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
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Stage 1-2 Periodontitis Group
Patients diagnosed with Stage 1 or Stage 2 periodontitis according to the 2017 World Workshop classification, characterized by interdental clinical attachment loss and radiographic bone loss.
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Gingival tissue samples are collected from all participants.
These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods.
Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
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Stage 3-4 Periodontitis Group
Patients diagnosed with Stage 3 or Stage 4 periodontitis, characterized by severe clinical attachment loss, deep probing depths, and significant radiographic bone loss potentially leading to tooth loss.
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Gingival tissue samples are collected from all participants.
These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods.
Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Thioredoxin Reductase 1 (TrxR1) mRNA Expression Levels
大体时间：Baseline (at the time of tissue collection)
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The expression levels of TrxR1 in gingival tissue are determined by quantitative real-time PCR (qPCR).
This measure evaluates the potential of TrxR1 as a biomarker for periodontitis severity.
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Baseline (at the time of tissue collection)
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Thioredoxin (TXN) mRNA Expression Levels
大体时间：Baseline (at the time of tissue collection)
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Evaluation of TXN gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin.
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Baseline (at the time of tissue collection)
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Thioredoxin-Interacting Protein (TXNIP) mRNA Expression Levels
大体时间：Baseline (at the time of tissue collection)
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Evaluation of TXNIP gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin. Time Frame: Baseline (at the time of tissue collection) |
Baseline (at the time of tissue collection)
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Malondialdehyde (MDA) Levels.
大体时间：Baseline (at the time of tissue collection).
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MDA levels in gingival tissue homogenates measured using the thiobarbituric acid reactive substances (TBARS) assay to evaluate lipid peroxidation.
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Baseline (at the time of tissue collection).
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Glutathione (GSH) Levels.
大体时间：Baseline (at the time of tissue collection).
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GSH levels in gingival tissue homogenates measured using spectroscopic methods to evaluate antioxidant status.
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Baseline (at the time of tissue collection).
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合作者和调查者
在这里您可以找到参与这项研究的人员和组织。
研究记录日期
这些日期跟踪向 ClinicalTrials.gov 提交研究记录和摘要结果的进度。研究记录和报告的结果由国家医学图书馆 (NLM) 审查，以确保它们在发布到公共网站之前符合特定的质量控制标准。
研究主要日期
学习开始 (实际的)
2023年8月30日
初级完成 (实际的)
2025年8月10日
研究完成 (实际的)
2025年10月15日
研究注册日期
首次提交
2026年5月14日
首先提交符合 QC 标准的
2026年5月14日
首次发布 (实际的)
2026年5月20日
研究记录更新
最后更新发布 (实际的)
2026年5月20日
上次提交的符合 QC 标准的更新
2026年5月14日
最后验证
2026年5月1日
更多信息
与本研究相关的术语
其他研究编号
- B.30.2.ATA.0.01.00/163
- TDK-2023-13289 (其他标识符：Atatürk University Scientific Research Projects)
计划个人参与者数据 (IPD)
计划共享个人参与者数据 (IPD)？
是的
IPD 计划说明
Individual participant data that underlie the results reported in this article, after de-identification, will be made available upon request.
IPD 共享时间框架
Data will be available beginning 6 months and ending 36 months following article publication.
IPD 共享访问标准
Proposals should be directed to turgdemir@hotmail.com.
To gain access, data requestors will need to sign a data access agreement.
IPD 共享支持信息类型
- 研究方案
- 树液
药物和器械信息、研究文件
研究美国 FDA 监管的药品
不
研究美国 FDA 监管的设备产品
不
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