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Gingival TrxR1 Expression in Periodontal Health and Periodontitis

14. maj 2026 opdateret af: Fuat Cetinkaya, Ataturk University

Thioredoxin Reductase 1 Levels in Gingival Tissue as an Oxidative Stress Biomarker in Periodontitis

This study aims to investigate the role of the thioredoxin system in the development and progression of periodontitis. Researchers will examine the levels of specific antioxidant markers, such as thioredoxin reductase 1 (TrxR1), glutathione (GSH), and malondialdehyde (MDA), in gingival tissue samples collected from healthy individuals and patients with different stages of periodontal disease. The goal is to determine if TrxR1 levels can serve as a potential biomarker for diagnosing the severity and activity of periodontitis.

Studieoversigt

Status

Afsluttet

Betingelser

Intervention / Behandling

Undersøgelsestype

Observationel

Tilmelding (Faktiske)

60

Kontakter og lokationer

Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.

Studiesteder

  • Tyrkiet (Türkiye)
      • Erzurum, Tyrkiet (Türkiye), 25240
        • Atatürk University Faculty of Dentistry

Deltagelseskriterier

Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.

Berettigelseskriterier

Aldre berettiget til at studere

  • Voksen
  • Ældre voksen

Tager imod sunde frivillige

Ja

Prøveudtagningsmetode

Ikke-sandsynlighedsprøve

Studiebefolkning

Patients visiting the Department of Periodontology at Atatürk University Faculty of Dentistry who meet the clinical criteria for health or periodontitis.

Beskrivelse

Inclusion Criteria:

  • Being between the ages of 18 and 70.
  • Having no known systemic disease.
  • Non-smokers.
  • No history of medication or supplement use in the last 3 months.
  • For the Periodontitis groups: Interdental clinical attachment loss (CAL), probing pocket depth (PPD), and radiographic bone loss according to 2017 World Workshop criteria.

Exclusion Criteria:

  • Smoking.
  • Pregnancy or breastfeeding.
  • Presence of a systemic disease (e.g., diabetes mellitus, hypertension, rheumatoid arthritis, cancer, thyroid disorders, kidney or lung diseases).
  • History of any periodontal surgical procedure within the last 6 months.
  • Use of medication or supplements within the last 3 months.

Studieplan

Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.

Hvordan er undersøgelsen tilrettelagt?

Design detaljer

Kohorter og interventioner

Gruppe / kohorte
Intervention / Behandling
Healthy Control Group
Systemically and periodontally healthy individuals with no history of bone loss, probing depths ≤ 3 mm, and bleeding on probing < 10%.
Andet: Gingival Tissue Analysis
Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
Stage 1-2 Periodontitis Group
Patients diagnosed with Stage 1 or Stage 2 periodontitis according to the 2017 World Workshop classification, characterized by interdental clinical attachment loss and radiographic bone loss.
Andet: Gingival Tissue Analysis
Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
Stage 3-4 Periodontitis Group
Patients diagnosed with Stage 3 or Stage 4 periodontitis, characterized by severe clinical attachment loss, deep probing depths, and significant radiographic bone loss potentially leading to tooth loss.
Andet: Gingival Tissue Analysis
Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.

Hvad måler undersøgelsen?

Primære resultatmål

Resultatmål
Foranstaltningsbeskrivelse
Tidsramme
Thioredoxin Reductase 1 (TrxR1) mRNA Expression Levels
Tidsramme: Baseline (at the time of tissue collection)
The expression levels of TrxR1 in gingival tissue are determined by quantitative real-time PCR (qPCR). This measure evaluates the potential of TrxR1 as a biomarker for periodontitis severity.
Baseline (at the time of tissue collection)

Sekundære resultatmål

Resultatmål
Foranstaltningsbeskrivelse
Tidsramme
Thioredoxin (TXN) mRNA Expression Levels
Tidsramme: Baseline (at the time of tissue collection)
Evaluation of TXN gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin.
Baseline (at the time of tissue collection)
Thioredoxin-Interacting Protein (TXNIP) mRNA Expression Levels
Tidsramme: Baseline (at the time of tissue collection)

Evaluation of TXNIP gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin.

Time Frame: Baseline (at the time of tissue collection)
Baseline (at the time of tissue collection)
Malondialdehyde (MDA) Levels.
Tidsramme: Baseline (at the time of tissue collection).
MDA levels in gingival tissue homogenates measured using the thiobarbituric acid reactive substances (TBARS) assay to evaluate lipid peroxidation.
Baseline (at the time of tissue collection).
Glutathione (GSH) Levels.
Tidsramme: Baseline (at the time of tissue collection).
GSH levels in gingival tissue homogenates measured using spectroscopic methods to evaluate antioxidant status.
Baseline (at the time of tissue collection).

Samarbejdspartnere og efterforskere

Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.

Sponsor

Ataturk University

Datoer for undersøgelser

Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.

Studer store datoer

Studiestart (Faktiske)

30. august 2023

Primær færdiggørelse (Faktiske)

10. august 2025

Studieafslutning (Faktiske)

15. oktober 2025

Datoer for studieregistrering

Først indsendt

14. maj 2026

Først indsendt, der opfyldte QC-kriterier

14. maj 2026

Først opslået (Faktiske)

20. maj 2026

Opdateringer af undersøgelsesjournaler

Sidste opdatering sendt (Faktiske)

20. maj 2026

Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier

14. maj 2026

Sidst verificeret

1. maj 2026

Mere information

Begreber relateret til denne undersøgelse

Nøgleord

Yderligere relevante MeSH-vilkår

Andre undersøgelses-id-numre

  • B.30.2.ATA.0.01.00/163
  • TDK-2023-13289 (Anden identifikator: Atatürk University Scientific Research Projects)

Plan for individuelle deltagerdata (IPD)

Planlægger du at dele individuelle deltagerdata (IPD)?

JA

IPD-planbeskrivelse

Individual participant data that underlie the results reported in this article, after de-identification, will be made available upon request.

IPD-delingstidsramme

Data will be available beginning 6 months and ending 36 months following article publication.

IPD-delingsadgangskriterier

Proposals should be directed to turgdemir@hotmail.com. To gain access, data requestors will need to sign a data access agreement.

IPD-deling Understøttende informationstype

  • STUDY_PROTOCOL
  • SAP

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