Chronic Presence of Epstein Barr Virus in Epithelial Cells From Gingiva is Associated With Periodontitis

The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Study Overview

• Status: Unknown
• Conditions: Aggressive Periodontitis; Chronic Periodontitis; Presence / Absence of EBV
• Intervention / Treatment: Procedure: dental root planing; Procedure: dental root planing

• Study Type: Interventional
• Enrollment (Anticipated): 30
• Phase: Not Applicable

Contacts and Locations

Study Locations

• France
  • Nice, France, 0600
    • Recruiting
    • Dr Severine VINCENT
    • Contact: Severine VINCENT, PH

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• 10 patients affected by chronic periodontitis
• 10 patients affected by aggressive periodontitis
• 10 healthy patients (orthodontics extraction)
• Subject who have read and understood the information note relative under investigation and who have signed the informed consent
• major patient

Exclusion Criteria:

• Patient with no contre-indication to periodontal treatment
• Patient with serious blood disease
• Patient who exhibits systemic condition incompatible with realization of the study
• Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants
• Patient who presents a buccal carcinoma treated with radiotherapy
• Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy
• Patient who presents a linguistic or psychic incapacity to understand information

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Health Services Research
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: chronic periodontitis	Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,[1] thus helping to establish a periodontium that is free of disease.[2]; Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.
Active Comparator: aggressive periodontitis	Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,[1] thus helping to establish a periodontium that is free of disease.[2]; Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.
Active Comparator: healthy patient (orthodontics extraction)	Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,[1] thus helping to establish a periodontium that is free of disease.[2]; Procedure: dental root planing; The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Percentage of gingival epithelial cells infected by EBV (by immunofluorescence and In situ Hybridization)	at time=0

Secondary Outcome Measures

Outcome Measure	Time Frame
Epithelial tissue infected by EBV (by immunofluorescence and in situ hybridization)	at time = 0
Presence / absence of latency and / or lytic proteins (by immunofluorescence and in situ hybridization)	at time = 0

Collaborators and Investigators

• Sponsor: Centre Hospitalier Universitaire de Nice
• Investigators: Principal Investigator: Severine VINCENT, PH, CHU de Nice Hôpital Saint Roch

Study record dates

Study Major Dates

• Study Start: January 1, 2011
• Primary Completion (Actual): June 1, 2011
• Study Completion (Anticipated): January 1, 2014

Study Registration Dates

• First Submitted: November 17, 2010
• First Submitted That Met QC Criteria: November 22, 2010
• First Posted (Estimate): November 23, 2010

Study Record Updates

• Last Update Posted (Estimate): December 18, 2012
• Last Update Submitted That Met QC Criteria: December 17, 2012
• Last Verified: November 1, 2010

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Periodontitis; Aggressive Periodontitis; Chronic Periodontitis
• Other Study ID Numbers: 10-AOI-01