The Effect of Non-Surgical Periodontal Treatment on Systemic Inflammatory Markers and Lipid Profile in Young Adults With Severe Periodontitis

The goal of this clinical study was to evaluate whether non-surgical treatment for severe periodontitis (gum disease) could improve body-wide inflammation and blood fat levels in young adults with severe periodontitis. The main questions it aimed to answer were:

Did non-surgical periodontal treatment improve gum health in young adults with severe periodontitis?

Did non-surgical periodontal treatment change body-wide inflammation and blood fat levels after treatment?

Researchers compared participants with severe periodontitis to healthy participants without periodontitis.

Participants with severe periodontitis:

• Received oral hygiene instructions, deep cleaning below the gumline, root surface cleaning, and removal of teeth that could not be saved
• Had full-mouth gum examinations
• Provided blood samples at the start of the study and again at 2 and 6 months after treatment

Healthy participants:

• Had gum examinations
• Provided blood samples for comparison at the start of the study

Study Overview

• Status: Completed
• Conditions: Periodontitis, Aggressive
• Intervention / Treatment: Procedure: Non-surgical periodontal treatment

• Study Type: Interventional
• Enrollment (Actual): 50
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Turkey (Türkiye)
  • Istanbul, Turkey (Türkiye)
    • Istanbul University, Faculty of Dentistry, Department of Periodontology

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Young adults aged 19 to 36 years
• At least 20 teeth present
• Clinical attachment loss of at least 5 mm affecting at least 8 teeth
• Bone loss around at least 3 teeth other than incisors and first molars

Exclusion Criteria:

• Systemic disease
• Pregnancy or lactation
• Current or former smoking
• Systemic conditions that could alter the host response
• Medical conditions requiring antibiotic prophylaxis during routine dental procedures
• Antibiotic therapy within the previous 6 months
• Previous periodontal treatment

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Healthy Group; The healthy control arm included systemically and periodontally healthy young adults with no periodontal or systemic disease who met the same exclusion criteria as the periodontitis group. Participants did not receive periodontal treatment. They underwent baseline full-mouth periodontal examination and blood sampling to provide healthy reference values.
Active Comparator: Periodontitis Group; The periodontitis arm included systemically healthy young adults with severe periodontitis. Participants underwent baseline full-mouth periodontal examination and blood sampling, received non-surgical periodontal treatment, and were re-evaluated at 2 and 6 months after treatment.	Procedure: Non-surgical periodontal treatment; Non-surgical periodontal treatment consisted of oral hygiene instruction, subgingival scaling, root planing, and extraction of unsalvageable teeth under local anesthesia. Treatment was completed within 1 week. Clinical periodontal measurements and blood samples were obtained at baseline and repeated at 2 and 6 months after completion of treatment.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
The change in serum high-sensitivity C-reactive protein (hs-CRP) concentration after periodontal treatment	This change is measured in serum, as mg/dl, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. The study was powered on the change in this parameter from baseline to 6 months after treatment.	6 months
The change in HDL cholesterol concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in LDL cholesterol concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in total cholesterol concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in triglyceride concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in albumin concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in von Willebrand factor (vWf) level after periodontal treatment	This parameter is measured in blood samples, as percentage (%), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in creatinine concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in fibrinogen concentration after periodontal treatment	This parameter is measured in blood samples, as mg/dL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in Factor VII level after periodontal treatment	This parameter is measured in blood samples, as percentage (%), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in interleukin-1 receptor (IL-1ra) antagonist concentration after periodontal treatment	This parameter is measured in blood samples, as pg/mL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in interleukin-6 (IL-6) level after periodontal treatment	This parameter is measured in blood samples, as pg/mL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in soluble tumor necrosis factor receptor I (sTNFRI) concentration after periodontal treatment	This parameter is measured in blood samples, as pg/mL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in soluble tumor necrosis factor receptor II concentration (sTNFRII) after periodontal treatment	This parameter is measured in blood samples, as pg/mL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months
The change in tissue plasminogen activator (tPA) concentration after periodontal treatment	This parameter is measured in blood samples, as ng/mL, recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the systemic biomarker panel examined to characterize the systemic inflammatory and metabolic profile of the study participants.	6 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
The change in sulcus bleeding index (SBI) after periodontal treatment	This parameter is measured by clinical examination, as a percentage (%), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the clinical periodontal parameters recorded to characterize the periodontal status of the study participants.	6 months
The change in plaque index (PI) after periodontal treatment	This parameter is measured by clinical examination, as a percentage (%), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the clinical periodontal parameters recorded to characterize the periodontal status of the study participants.	6 months
The change in clinical attachment level (CAL) after periodontal treatment	This parameter is measured by clinical examination, in millimeters (mm), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the clinical periodontal parameters recorded to characterize the periodontal status of the study participants.	6 months
The change in probing pocket depth (PPD) after periodontal treatment	This parameter is measured by clinical examination, in millimeters (mm), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the clinical periodontal parameters recorded to characterize the periodontal status of the study participants.	6 months
The change in level of gingival margin (LGM) after periodontal treatment	This parameter is measured by clinical examination, in millimeters (mm), recorded at baseline, 2 and 6 months after non-surgical periodontal treatment. It is part of the clinical periodontal parameters recorded to characterize the periodontal status of the study participants.	6 months

Collaborators and Investigators

• Sponsor: Istanbul University

Study record dates

Study Major Dates

• Study Start (Actual): July 1, 2006
• Primary Completion (Actual): December 1, 2009
• Study Completion (Actual): June 1, 2010

Study Registration Dates

• First Submitted: March 13, 2026
• First Submitted That Met QC Criteria: April 9, 2026
• First Posted (Actual): April 13, 2026

Study Record Updates

• Last Update Posted (Actual): April 13, 2026
• Last Update Submitted That Met QC Criteria: April 9, 2026
• Last Verified: April 1, 2026

More Information

Terms related to this study

• Keywords: Inflammation; Periodontitis; Periodontal Debridement; Systemic Biomarkers
• Additional Relevant MeSH Terms: Periodontal Diseases; Mouth Diseases; Stomatognathic Diseases; Pathologic Processes; Pathological Conditions, Signs and Symptoms; Periodontitis; Inflammation; Aggressive Periodontitis
• Other Study ID Numbers: 2006/1469

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No