Eccentric Exercise and Oxidative Stress

November 13, 2012 updated by: Dr. Anastasios Theodorou, European University Cyprus

The Effect of Eccentric Exercise on Oxidative Stress in the Elderly

The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.

Study Type

Interventional

Enrollment (Actual)

20

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Cyprus
      • Nicosia, Cyprus, 1516
        • European University Cyprus

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 70 years (Adult, Older Adult)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Male

Description

Inclusion Criteria:

  • subject provides written informed consent
  • physiological health profile
  • stable at their anthropometric characteristics for at least the last year

Exclusion Criteria:

  • muscle disease
  • prior history of musculoskeletal injury to the lower limbs
  • smokers
  • consumed any nutritional supplement the last 3 months
  • performed intense eccentric exercise the last 6 months
  • non Caucasian

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Supportive Care
  • Allocation: Non-Randomized
  • Interventional Model: Parallel Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Young group
Participants will underwent two isokinetic eccentric exercise sessions
Other: Exercise
Participants will perform two isokinetic eccentric exercise bouts
Experimental: Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
Other: Exercise
Participants will perform two isokinetic eccentric exercise bouts

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Time Frame
Muscle torque (torque)
Time Frame: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Range of motion, ROM (degrees)
Time Frame: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Delayed onset muscle soreness, DOMS (scale 1-10)
Time Frame: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Creatine kinase, CK (activity IU)
Time Frame: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Secondary Outcome Measures

Outcome Measure
Time Frame
F2-isoprostanes (pg/mg creatinine)
Time Frame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Protein carbonyls (nmol/mg protein)
Time Frame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glutathione, GSH (μmol/g Hb)
Time Frame: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Catalase (U/g Hb)
Time Frame: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Superoxide dismutase, SOD (U/g Hb)
Time Frame: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glutathione peroxidase, GPx (U/g Hb)
Time Frame: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
Time Frame: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Uric acid (mM)
Time Frame: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Albumin (μM)
Time Frame: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Bilirubin (μM)
Time Frame: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

European University Cyprus

Collaborators

Aristotle University Of Thessaloniki

Investigators

  • Study Chair: Michalis G Nikolaidis, PhD, Aristotle University Of Thessaloniki

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

May 1, 2012

Primary Completion (Actual)

July 1, 2012

Study Completion (Actual)

July 1, 2012

Study Registration Dates

First Submitted

November 6, 2012

First Submitted That Met QC Criteria

November 13, 2012

First Posted (Estimate)

November 20, 2012

Study Record Updates

Last Update Posted (Estimate)

November 20, 2012

Last Update Submitted That Met QC Criteria

November 13, 2012

Last Verified

November 1, 2012

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • EUC-017
  • EUC-019 (Other Grant/Funding Number: European University Cyprus)

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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