Eccentric Exercise and Oxidative Stress
13 november 2012 uppdaterad av: Dr. Anastasios Theodorou, European University Cyprus
The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Studieöversikt
Status
Avslutad
Betingelser
Intervention / Behandling
Detaljerad beskrivning
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly.
A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging.
Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks.
Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Studietyp
Interventionell
Inskrivning (Faktisk)
20
Fas
- Inte tillämpbar
Kontakter och platser
Det här avsnittet innehåller kontaktuppgifter för dem som genomför studien och information om var denna studie genomförs.
Studieorter
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Nicosia, Cypern, 1516
- European University Cyprus
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Deltagandekriterier
Forskare letar efter personer som passar en viss beskrivning, så kallade behörighetskriterier. Några exempel på dessa kriterier är en persons allmänna hälsotillstånd eller tidigare behandlingar.
Urvalskriterier
Åldrar som är berättigade till studier
18 år till 70 år (Vuxen, Äldre vuxen)
Tar emot friska volontärer
Ja
Kön som är behöriga för studier
Manlig
Beskrivning
Inclusion Criteria:
- subject provides written informed consent
- physiological health profile
- stable at their anthropometric characteristics for at least the last year
Exclusion Criteria:
- muscle disease
- prior history of musculoskeletal injury to the lower limbs
- smokers
- consumed any nutritional supplement the last 3 months
- performed intense eccentric exercise the last 6 months
- non Caucasian
Studieplan
Det här avsnittet ger detaljer om studieplanen, inklusive hur studien är utformad och vad studien mäter.
Hur är studien utformad?
Designdetaljer
- Primärt syfte: Stödjande vård
- Tilldelning: Icke-randomiserad
- Interventionsmodell: Parallellt uppdrag
- Maskning: Ingen (Open Label)
Vapen och interventioner
Deltagargrupp / Arm |
Intervention / Behandling |
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Experimentell: Young group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Experimentell: Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Vad mäter studien?
Primära resultatmått
Resultatmått |
Tidsram |
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Muscle torque (torque)
Tidsram: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Range of motion, ROM (degrees)
Tidsram: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Delayed onset muscle soreness, DOMS (scale 1-10)
Tidsram: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Creatine kinase, CK (activity IU)
Tidsram: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Sekundära resultatmått
Resultatmått |
Tidsram |
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F2-isoprostanes (pg/mg creatinine)
Tidsram: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Protein carbonyls (nmol/mg protein)
Tidsram: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione, GSH (μmol/g Hb)
Tidsram: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Catalase (U/g Hb)
Tidsram: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Superoxide dismutase, SOD (U/g Hb)
Tidsram: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione peroxidase, GPx (U/g Hb)
Tidsram: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
Tidsram: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Uric acid (mM)
Tidsram: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Albumin (μM)
Tidsram: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Bilirubin (μM)
Tidsram: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Samarbetspartners och utredare
Det är här du hittar personer och organisationer som är involverade i denna studie.
Sponsor
Samarbetspartners
Utredare
- Studiestol: Michalis G Nikolaidis, PhD, Aristotle University Of Thessaloniki
Publikationer och användbara länkar
Den som ansvarar för att lägga in information om studien tillhandahåller frivilligt dessa publikationer. Dessa kan handla om allt som har med studien att göra.
Allmänna publikationer
- Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.
- Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.
- Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.
- Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.
- Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.
Studieavstämningsdatum
Dessa datum spårar framstegen för inlämningar av studieposter och sammanfattande resultat till ClinicalTrials.gov. Studieposter och rapporterade resultat granskas av National Library of Medicine (NLM) för att säkerställa att de uppfyller specifika kvalitetskontrollstandarder innan de publiceras på den offentliga webbplatsen.
Studera stora datum
Studiestart
1 maj 2012
Primärt slutförande (Faktisk)
1 juli 2012
Avslutad studie (Faktisk)
1 juli 2012
Studieregistreringsdatum
Först inskickad
6 november 2012
Först inskickad som uppfyllde QC-kriterierna
13 november 2012
Första postat (Uppskatta)
20 november 2012
Uppdateringar av studier
Senaste uppdatering publicerad (Uppskatta)
20 november 2012
Senaste inskickade uppdateringen som uppfyllde QC-kriterierna
13 november 2012
Senast verifierad
1 november 2012
Mer information
Termer relaterade till denna studie
Nyckelord
Andra studie-ID-nummer
- EUC-017
- EUC-019 (Annat bidrag/finansieringsnummer: European University Cyprus)
Denna information hämtades direkt från webbplatsen clinicaltrials.gov utan några ändringar. Om du har några önskemål om att ändra, ta bort eller uppdatera dina studieuppgifter, vänligen kontakta register@clinicaltrials.gov. Så snart en ändring har implementerats på clinicaltrials.gov, kommer denna att uppdateras automatiskt även på vår webbplats .
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