Eccentric Exercise and Oxidative Stress
13 november 2012 bijgewerkt door: Dr. Anastasios Theodorou, European University Cyprus
The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Studie Overzicht
Toestand
Voltooid
Conditie
Interventie / Behandeling
Gedetailleerde beschrijving
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly.
A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging.
Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks.
Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Studietype
Ingrijpend
Inschrijving (Werkelijk)
20
Fase
- Niet toepasbaar
Contacten en locaties
In dit gedeelte vindt u de contactgegevens van degenen die het onderzoek uitvoeren en informatie over waar dit onderzoek wordt uitgevoerd.
Studie Locaties
-
-
-
Nicosia, Cyprus, 1516
- European University Cyprus
-
-
Deelname Criteria
Onderzoekers zoeken naar mensen die aan een bepaalde beschrijving voldoen, de zogenaamde geschiktheidscriteria. Enkele voorbeelden van deze criteria zijn iemands algemene gezondheidstoestand of eerdere behandelingen.
Geschiktheidscriteria
Leeftijden die in aanmerking komen voor studie
18 jaar tot 70 jaar (Volwassen, Oudere volwassene)
Accepteert gezonde vrijwilligers
Ja
Geslachten die in aanmerking komen voor studie
Mannelijk
Beschrijving
Inclusion Criteria:
- subject provides written informed consent
- physiological health profile
- stable at their anthropometric characteristics for at least the last year
Exclusion Criteria:
- muscle disease
- prior history of musculoskeletal injury to the lower limbs
- smokers
- consumed any nutritional supplement the last 3 months
- performed intense eccentric exercise the last 6 months
- non Caucasian
Studie plan
Dit gedeelte bevat details van het studieplan, inclusief hoe de studie is opgezet en wat de studie meet.
Hoe is de studie opgezet?
Ontwerpdetails
- Primair doel: Ondersteunende zorg
- Toewijzing: Niet-gerandomiseerd
- Interventioneel model: Parallelle opdracht
- Masker: Geen (open label)
Wapens en interventies
Deelnemersgroep / Arm |
Interventie / Behandeling |
|---|---|
|
Experimenteel: Young group
Participants will underwent two isokinetic eccentric exercise sessions
|
Participants will perform two isokinetic eccentric exercise bouts
|
|
Experimenteel: Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
|
Participants will perform two isokinetic eccentric exercise bouts
|
Wat meet het onderzoek?
Primaire uitkomstmaten
Uitkomstmaat |
Tijdsspanne |
|---|---|
|
Muscle torque (torque)
Tijdsspanne: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Range of motion, ROM (degrees)
Tijdsspanne: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Delayed onset muscle soreness, DOMS (scale 1-10)
Tijdsspanne: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Creatine kinase, CK (activity IU)
Tijdsspanne: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Secundaire uitkomstmaten
Uitkomstmaat |
Tijdsspanne |
|---|---|
|
F2-isoprostanes (pg/mg creatinine)
Tijdsspanne: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Protein carbonyls (nmol/mg protein)
Tijdsspanne: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Glutathione, GSH (μmol/g Hb)
Tijdsspanne: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Catalase (U/g Hb)
Tijdsspanne: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Superoxide dismutase, SOD (U/g Hb)
Tijdsspanne: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Glutathione peroxidase, GPx (U/g Hb)
Tijdsspanne: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
Tijdsspanne: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Uric acid (mM)
Tijdsspanne: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Albumin (μM)
Tijdsspanne: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
|
Bilirubin (μM)
Tijdsspanne: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
|
Medewerkers en onderzoekers
Hier vindt u mensen en organisaties die betrokken zijn bij dit onderzoek.
Sponsor
Medewerkers
Onderzoekers
- Studie stoel: Michalis G Nikolaidis, PhD, Aristotle University Of Thessaloniki
Publicaties en nuttige links
De persoon die verantwoordelijk is voor het invoeren van informatie over het onderzoek stelt deze publicaties vrijwillig ter beschikking. Dit kan gaan over alles wat met het onderzoek te maken heeft.
Algemene publicaties
- Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.
- Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.
- Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.
- Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.
- Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.
Studie record data
Deze datums volgen de voortgang van het onderzoeksdossier en de samenvatting van de ingediende resultaten bij ClinicalTrials.gov. Studieverslagen en gerapporteerde resultaten worden beoordeeld door de National Library of Medicine (NLM) om er zeker van te zijn dat ze voldoen aan specifieke kwaliteitscontrolenormen voordat ze op de openbare website worden geplaatst.
Bestudeer belangrijke data
Studie start
1 mei 2012
Primaire voltooiing (Werkelijk)
1 juli 2012
Studie voltooiing (Werkelijk)
1 juli 2012
Studieregistratiedata
Eerst ingediend
6 november 2012
Eerst ingediend dat voldeed aan de QC-criteria
13 november 2012
Eerst geplaatst (Schatting)
20 november 2012
Updates van studierecords
Laatste update geplaatst (Schatting)
20 november 2012
Laatste update ingediend die voldeed aan QC-criteria
13 november 2012
Laatst geverifieerd
1 november 2012
Meer informatie
Termen gerelateerd aan deze studie
Trefwoorden
Andere studie-ID-nummers
- EUC-017
- EUC-019 (Ander subsidie-/financieringsnummer: European University Cyprus)
Deze informatie is zonder wijzigingen rechtstreeks van de website clinicaltrials.gov gehaald. Als u verzoeken heeft om uw onderzoeksgegevens te wijzigen, te verwijderen of bij te werken, neem dan contact op met register@clinicaltrials.gov. Zodra er een wijziging wordt doorgevoerd op clinicaltrials.gov, wordt deze ook automatisch bijgewerkt op onze website .