Eccentric Exercise and Oxidative Stress
13. november 2012 opdateret af: Dr. Anastasios Theodorou, European University Cyprus
The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Studieoversigt
Status
Afsluttet
Betingelser
Intervention / Behandling
Detaljeret beskrivelse
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly.
A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging.
Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks.
Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Undersøgelsestype
Interventionel
Tilmelding (Faktiske)
20
Fase
- Ikke anvendelig
Kontakter og lokationer
Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.
Studiesteder
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Nicosia, Cypern, 1516
- European University Cyprus
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Deltagelseskriterier
Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.
Berettigelseskriterier
Aldre berettiget til at studere
18 år til 70 år (Voksen, Ældre voksen)
Tager imod sunde frivillige
Ja
Køn, der er berettiget til at studere
Han
Beskrivelse
Inclusion Criteria:
- subject provides written informed consent
- physiological health profile
- stable at their anthropometric characteristics for at least the last year
Exclusion Criteria:
- muscle disease
- prior history of musculoskeletal injury to the lower limbs
- smokers
- consumed any nutritional supplement the last 3 months
- performed intense eccentric exercise the last 6 months
- non Caucasian
Studieplan
Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Støttende pleje
- Tildeling: Ikke-randomiseret
- Interventionel model: Parallel tildeling
- Maskning: Ingen (Åben etiket)
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
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Eksperimentel: Young group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Eksperimentel: Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Tidsramme |
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Muscle torque (torque)
Tidsramme: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Range of motion, ROM (degrees)
Tidsramme: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Delayed onset muscle soreness, DOMS (scale 1-10)
Tidsramme: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Creatine kinase, CK (activity IU)
Tidsramme: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Sekundære resultatmål
Resultatmål |
Tidsramme |
|---|---|
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F2-isoprostanes (pg/mg creatinine)
Tidsramme: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Protein carbonyls (nmol/mg protein)
Tidsramme: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione, GSH (μmol/g Hb)
Tidsramme: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Catalase (U/g Hb)
Tidsramme: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Superoxide dismutase, SOD (U/g Hb)
Tidsramme: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione peroxidase, GPx (U/g Hb)
Tidsramme: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
Tidsramme: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Uric acid (mM)
Tidsramme: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Albumin (μM)
Tidsramme: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Bilirubin (μM)
Tidsramme: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Samarbejdspartnere og efterforskere
Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.
Sponsor
Samarbejdspartnere
Efterforskere
- Studiestol: Michalis G Nikolaidis, PhD, Aristotle University Of Thessaloniki
Publikationer og nyttige links
Den person, der er ansvarlig for at indtaste oplysninger om undersøgelsen, leverer frivilligt disse publikationer. Disse kan handle om alt relateret til undersøgelsen.
Generelle publikationer
- Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.
- Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.
- Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.
- Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.
- Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.
Datoer for undersøgelser
Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.
Studer store datoer
Studiestart
1. maj 2012
Primær færdiggørelse (Faktiske)
1. juli 2012
Studieafslutning (Faktiske)
1. juli 2012
Datoer for studieregistrering
Først indsendt
6. november 2012
Først indsendt, der opfyldte QC-kriterier
13. november 2012
Først opslået (Skøn)
20. november 2012
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Skøn)
20. november 2012
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
13. november 2012
Sidst verificeret
1. november 2012
Mere information
Begreber relateret til denne undersøgelse
Nøgleord
Andre undersøgelses-id-numre
- EUC-017
- EUC-019 (Andet bevillings-/finansieringsnummer: European University Cyprus)
Disse oplysninger blev hentet direkte fra webstedet clinicaltrials.gov uden ændringer. Hvis du har nogen anmodninger om at ændre, fjerne eller opdatere dine undersøgelsesoplysninger, bedes du kontakte register@clinicaltrials.gov. Så snart en ændring er implementeret på clinicaltrials.gov, vil denne også blive opdateret automatisk på vores hjemmeside .