Eccentric Exercise and Oxidative Stress
13. November 2012 aktualisiert von: Dr. Anastasios Theodorou, European University Cyprus
The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Studienübersicht
Status
Abgeschlossen
Bedingungen
Intervention / Behandlung
Detaillierte Beschreibung
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly.
A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging.
Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks.
Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Studientyp
Interventionell
Einschreibung (Tatsächlich)
20
Phase
- Unzutreffend
Kontakte und Standorte
Dieser Abschnitt enthält die Kontaktdaten derjenigen, die die Studie durchführen, und Informationen darüber, wo diese Studie durchgeführt wird.
Studienorte
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Nicosia, Zypern, 1516
- European University Cyprus
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Teilnahmekriterien
Forscher suchen nach Personen, die einer bestimmten Beschreibung entsprechen, die als Auswahlkriterien bezeichnet werden. Einige Beispiele für diese Kriterien sind der allgemeine Gesundheitszustand einer Person oder frühere Behandlungen.
Zulassungskriterien
Studienberechtigtes Alter
18 Jahre bis 70 Jahre (Erwachsene, Älterer Erwachsener)
Akzeptiert gesunde Freiwillige
Ja
Studienberechtigte Geschlechter
Männlich
Beschreibung
Inclusion Criteria:
- subject provides written informed consent
- physiological health profile
- stable at their anthropometric characteristics for at least the last year
Exclusion Criteria:
- muscle disease
- prior history of musculoskeletal injury to the lower limbs
- smokers
- consumed any nutritional supplement the last 3 months
- performed intense eccentric exercise the last 6 months
- non Caucasian
Studienplan
Dieser Abschnitt enthält Einzelheiten zum Studienplan, einschließlich des Studiendesigns und der Messung der Studieninhalte.
Wie ist die Studie aufgebaut?
Designdetails
- Hauptzweck: Unterstützende Pflege
- Zuteilung: Nicht randomisiert
- Interventionsmodell: Parallele Zuordnung
- Maskierung: Keine (Offenes Etikett)
Waffen und Interventionen
Teilnehmergruppe / Arm |
Intervention / Behandlung |
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Experimental: Young group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Experimental: Elderly group
Participants will underwent two isokinetic eccentric exercise sessions
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Participants will perform two isokinetic eccentric exercise bouts
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Was misst die Studie?
Primäre Ergebnismessungen
Ergebnis Maßnahme |
Zeitfenster |
|---|---|
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Muscle torque (torque)
Zeitfenster: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Range of motion, ROM (degrees)
Zeitfenster: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Delayed onset muscle soreness, DOMS (scale 1-10)
Zeitfenster: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Creatine kinase, CK (activity IU)
Zeitfenster: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Sekundäre Ergebnismessungen
Ergebnis Maßnahme |
Zeitfenster |
|---|---|
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F2-isoprostanes (pg/mg creatinine)
Zeitfenster: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Protein carbonyls (nmol/mg protein)
Zeitfenster: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione, GSH (μmol/g Hb)
Zeitfenster: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Catalase (U/g Hb)
Zeitfenster: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Superoxide dismutase, SOD (U/g Hb)
Zeitfenster: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glutathione peroxidase, GPx (U/g Hb)
Zeitfenster: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
Zeitfenster: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Uric acid (mM)
Zeitfenster: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Albumin (μM)
Zeitfenster: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Bilirubin (μM)
Zeitfenster: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
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Mitarbeiter und Ermittler
Hier finden Sie Personen und Organisationen, die an dieser Studie beteiligt sind.
Sponsor
Mitarbeiter
Ermittler
- Studienstuhl: Michalis G Nikolaidis, PhD, Aristotle University Of Thessaloniki
Publikationen und hilfreiche Links
Die Bereitstellung dieser Publikationen erfolgt freiwillig durch die für die Eingabe von Informationen über die Studie verantwortliche Person. Diese können sich auf alles beziehen, was mit dem Studium zu tun hat.
Allgemeine Veröffentlichungen
- Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.
- Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.
- Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.
- Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.
- Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.
Studienaufzeichnungsdaten
Diese Daten verfolgen den Fortschritt der Übermittlung von Studienaufzeichnungen und zusammenfassenden Ergebnissen an ClinicalTrials.gov. Studienaufzeichnungen und gemeldete Ergebnisse werden von der National Library of Medicine (NLM) überprüft, um sicherzustellen, dass sie bestimmten Qualitätskontrollstandards entsprechen, bevor sie auf der öffentlichen Website veröffentlicht werden.
Haupttermine studieren
Studienbeginn
1. Mai 2012
Primärer Abschluss (Tatsächlich)
1. Juli 2012
Studienabschluss (Tatsächlich)
1. Juli 2012
Studienanmeldedaten
Zuerst eingereicht
6. November 2012
Zuerst eingereicht, das die QC-Kriterien erfüllt hat
13. November 2012
Zuerst gepostet (Schätzen)
20. November 2012
Studienaufzeichnungsaktualisierungen
Letztes Update gepostet (Schätzen)
20. November 2012
Letztes eingereichtes Update, das die QC-Kriterien erfüllt
13. November 2012
Zuletzt verifiziert
1. November 2012
Mehr Informationen
Begriffe im Zusammenhang mit dieser Studie
Schlüsselwörter
Andere Studien-ID-Nummern
- EUC-017
- EUC-019 (Andere Zuschuss-/Finanzierungsnummer: European University Cyprus)
Diese Informationen wurden ohne Änderungen direkt von der Website clinicaltrials.gov abgerufen. Wenn Sie Ihre Studiendaten ändern, entfernen oder aktualisieren möchten, wenden Sie sich bitte an register@clinicaltrials.gov. Sobald eine Änderung auf clinicaltrials.gov implementiert wird, wird diese automatisch auch auf unserer Website aktualisiert .
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