Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage

The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

Study Overview

• Status: Unknown
• Conditions: Sleep; Sleep Deprivation
• Intervention / Treatment: Other: Sleep deprivation; Other: Normal sleep

Detailed Description

The blood parameters analyzed will be:

• Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol.
• Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours.
• GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00.
• TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day.

It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.

• Study Type: Interventional
• Enrollment (Actual): 10
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • Sao Paulo, Brazil, 04020-050
    • Associação Fundo de Incentivo à Pesquisa

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 31 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• healthy subjects
• sedentary
• sleep duration between 6-8 hours peer night

Exclusion Criteria:

• smokers
• use vitamin supplements, like C and E
• drink two doses of alcohol/day or > 4 doses in one occasion
• use of anti-inflammatory drugs
• antilipidemic drugs
• anemia
• muscle or joint diseases
• cardiovascular diseases
• diabetes
• renal diseases
• sleep disturbances
• breathing disorders

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Sleep deprivation; Exercise induced-muscle damage protocol followed by 60h of sleep deprivation (two nights) and one night of normal sleep (rebound).	Other: Sleep deprivation; This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and than will keep awake for 60 hours (2 nights), followed by 1 night of sleep, all inside the lab. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.; Other Names: Sleep and muscle recovery
Experimental: Normal sleep; Exercise induced-muscle damage protocol followed by 3 nights of normal sleep	Other: Normal sleep; This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and and will be able to sleep the 3 nights. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Change from baseline in isometric strength	Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage

Secondary Outcome Measures

Outcome Measure	Time Frame
Area Under Curve for serum total and free testosterone, IGF-1, and cortisol	Blood samples will be collected every 2 hours, for 24 hours.

Other Outcome Measures

Outcome Measure	Time Frame
Area Under Curve for serum pro and anti-inflammatory cytokines	Blood samples will be collected every 4 hours, for 24 hours.

Collaborators and Investigators

• Sponsor: Federal University of São Paulo
• Collaborators: Fundação de Amparo à Pesquisa do Estado de São Paulo

Study record dates

Study Major Dates

• Study Start: July 1, 2013
• Primary Completion (Actual): December 1, 2013

Study Registration Dates

• First Submitted: March 6, 2014
• First Submitted That Met QC Criteria: March 6, 2014
• First Posted (Estimate): March 10, 2014

Study Record Updates

• Last Update Posted (Estimate): March 11, 2014
• Last Update Submitted That Met QC Criteria: March 10, 2014
• Last Verified: March 1, 2014

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Mental Disorders; Nervous System Diseases; Dyssomnias; Sleep Wake Disorders; Neurologic Manifestations; Sleep Deprivation
• Other Study ID Numbers: 2010070050