Effects of Disrupting Prolonged Sitting With Different Physical Activity Protocols on Metabolic Risk Factors in Sedentary Adults

Great controversy exists with respect to the optimal or the minimum volume, intensity, and frequency of physical activity capable of attenuating the hazards of prolonged sitting on the metabolic profile. Thus, our study aims to comprehensively investigate the effects of disrupting prolonged sitting with different physical activity protocols on metabolic risk factors in sedentary males.

Ten sedentary males will take part in this randomized cross-over trial consisting of four 27-h conditions. All conditions will be identical except for the physical activity: prolonged sitting intervention (SIT) participants will sit continuously for 9 hours; prolonged sitting+interval standing intervention (STAND), participants will stand for 15 min every 30 minutes (total 270 min) during the 9 hours of sitting; prolonged sitting+moderate-intensity exercise bout (MVPA) participants will perform a 30-min moderate-intensity exercise bout on a treadmill (energy-matched to STAND), after which they will sit for the remaining time; and prolonged sitting+moderate-intensity exercise bout +interval standing intervention (MVPA-STAND), participants will perform a 30-min moderate-intensity exercise bout on a treadmill (energy-matched to STAND), after which they will stand for 15 min every 30 minutes (total 240 min) during the remaining 8 hours of sitting. Blood glucose, insulin, lipids and cytokines will be determined.

The investigators expect that disrupting prolonged sitting with intermittent standing and a moderate-exercise bout will positively affect the metabolic profile of the participants. Furthermore, we will investigate if combined, these strategies will have an additive effect.

Study Overview

• Status: Unknown
• Conditions: Prolonged Sitting; Breaking Prolonged Sitting With Physical Activity
• Intervention / Treatment: Behavioral: Breaking prolonged sitting with physical activity

• Study Type: Interventional
• Enrollment (Anticipated): 10
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Fabiana Benatti; Phone Number: +45 35450898; Email: fabiana.braga.benatti@regionh.dk

Study Locations

• Denmark
  • Copenhagen, Denmark, 2100
    • Recruiting
    • Centre of Inflammation and Metabolism
    • Contact: Inge Holm
    • Principal Investigator: Bente Pedersen, PhD
    • Sub-Investigator: Fabiana Benatti, PhD

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 25 years to 55 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Males,
• age 25-55 y,
• BMI >18 and <35 kg/m2

Exclusion Criteria:

• Clinically diagnosed diabetes,
• dyslipidaemia,
• hypertension,
• use of glucose- and/or lipid-lowering medication,
• smoking,
• evidence of thyroid, liver, lung, heart or kidney disease,
• non-sedentary occupation and primary means of commuting to work (i.e., cycling) in the last 4 months,
• VO2max levels above the considered average fitness according to age,
• contraindication to increased levels of physical activity.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Prolonged sitting; 9 h of prolonged sitting
Experimental: Prolonged sitting + interval standing bouts; Stand bout for 15-min every 30 minutes during the 9 hours of sitting	Behavioral: Breaking prolonged sitting with physical activity
Experimental: Moderate exercise bout + Prolonged sitting; 30-min moderate-intensity exercise bout followed by 8 h of sitting	Behavioral: Breaking prolonged sitting with physical activity
Experimental: Moderate exercise bout + Prolonged sitting +Standing bouts; 30-min moderate-intensity exercise bout and stand bout for 15-min every 30 minutes during the remaining 8 hours of sitting	Behavioral: Breaking prolonged sitting with physical activity

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Area under the curve of glucose and insulin postprandial responses	Glucose and insulin levels will be assessed 10 min before, and 30, 60, 120 and 180 min after each main meal. These postprandial responses will be defined as the area under the curve (AUC) assessed over the 3-h period after each main meal (3-h AUC) and the cumulative AUC after all main meals (12-h AUC).	27 h

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Glucose levels	Glucose incursions will measured using the glucose monitoring (CGM) system throughout the 27 hours. Mean, maximum and minimum glucose values during the 27h will be the outcome measures (mmol/l).	27 hours
Area under the curve of lipid postprandial responses	Lipid levels will be assessed 10 min before, and 30, 60, 120 and 180 min after each main meal. These postprandial responses will be defined as the area under the curve (AUC) assessed over the 3-h period after each main meal (3-h AUC) and the cumulative AUC after all main meals (12-h AUC).	27 h

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Area under the curve of cytokine postprandial responses	Cytokine levels (TNF-alpha, IL-6, IL-10, and IL1-ra) will be assessed 10 min before, and 30, 60, 120 and 180 min after each main meal. These postprandial responses will be defined as the area under the curve (AUC) assessed over the 3-h period after each main meal (3-h AUC) and the cumulative AUC after all main meals (12-h AUC).	27 h

Collaborators and Investigators

• Sponsor: Rigshospitalet, Denmark
• Collaborators: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

Study record dates

Study Major Dates

• Study Start: August 1, 2014
• Primary Completion (Anticipated): June 1, 2016
• Study Completion (Anticipated): December 1, 2016

Study Registration Dates

• First Submitted: August 7, 2014
• First Submitted That Met QC Criteria: August 11, 2014
• First Posted (Estimate): August 13, 2014

Study Record Updates

• Last Update Posted (Estimate): January 13, 2016
• Last Update Submitted That Met QC Criteria: January 12, 2016
• Last Verified: January 1, 2016

More Information

Terms related to this study

• Other Study ID Numbers: ProlongedSitting