Breaking Sitting and Metabolic Health in Sedentary Overweight and Obese Adults

Acute Effects of Breaking Prolonged Sitting on Postprandial Glycaemic Response, Incretin Hormones Secretion and Eating Behaviour in Overweight and Obese Adults

The purpose of this project is to investigate the effects of breaking up prolonged sitting on postprandial metabolic responses, gut hormones secretion and energy balance in sedentary overweight and obese adults.

Study Overview

• Status: Completed
• Conditions: Energy Balance; Metabolic Health; Gut Hormones
• Intervention / Treatment: Behavioral: Prolonged sitting; Behavioral: Breaking sitting

Detailed Description

Two trials are included in this project - Prolonged sitting and Breaking sitting.

In the Prolonged sitting trial, after consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min. In the Breaking sitting trial, after consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min. Blood samples were collected in a regular pattern throughout the trials. Energy balance (e.g., energy intake and spontaneous physical activity) was assessed using food diary and an accelerometer until midnight in the trial days once participants left laboratory.

• Study Type: Interventional
• Enrollment (Actual): 13
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Taiwan
  • Taipei, Taiwan
    • National Taiwan Normal University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 45 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Physical inactive males and females
• Aged between 20 and 45 years
• Overweight and obese healthy adults (waist circumference greater than 90 cm for males and 80 cm for females)
• Weight stable for more than 3 months (no change in weight +/- 3%)
• Non-smoker
• Able to walk comfortably on a treadmill

Exclusion Criteria:

• Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia
• Waist circumference < 90 cm for male and < 80 cm for female
• Taking medications that may influence lipid or carbohydrate metabolism or immune system function
• Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Prolonged sitting	Behavioral: Prolonged sitting; After consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min.
Experimental: Breaking sitting; Breaking prolonged sitting	Behavioral: Breaking sitting; After consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Gut hormones responses	Changes in blood gut hormones responses (e.g., GLP-1, PYY and GIP concentrations measured in pmol/L) between trials	320 minutes

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Incremental area under curve (iAUC) for metabolic health	Changes in incremental area under curve (iAUC) for insulin and glucose between trials	320 minutes
Visual analogue scale (VAS)	Changes in VAS from 0-100 mm (e.g., appetite and mood, etc.) between trials	320 minutes
Energy intake	Changes in energy intake (e.g, using a food diary to record) after leaving the laboratory until midnight	600 minutes after leaving the labalotory
Physical activity level	Changes in physical activity level (e.g., using an accelerometer to record) after leaving the laboratory until midnight	600 minutes after leaving the labalotory
Lipid profile	Changes in blood lipid profile (e.g., TAG and cholesterol, etc.) between trials	320 minutes
Blood pressure	Changes in blood pressure (e.g., diastolic and systolic blood pressure) between trials	320 minutes
Computerized cognitive testing	Changes in cognitive function (e.g., accuracy and reaction time) between trials	320 minutes

Collaborators and Investigators

• Sponsor: National Taiwan Normal University
• Investigators: Principal Investigator: Chen, PhD, National Taiwan Normal University

Study record dates

Study Major Dates

• Study Start (Actual): December 29, 2020
• Primary Completion (Actual): March 1, 2023
• Study Completion (Actual): March 1, 2023

Study Registration Dates

• First Submitted: March 15, 2022
• First Submitted That Met QC Criteria: March 23, 2022
• First Posted (Actual): April 1, 2022

Study Record Updates

• Last Update Posted (Actual): May 1, 2024
• Last Update Submitted That Met QC Criteria: April 29, 2024
• Last Verified: April 1, 2024

More Information

Terms related to this study

• Keywords: GLP-1; Energy balance; PYY; Postprandial glycaemia; Breaking sitting
• Additional Relevant MeSH Terms: Overnutrition; Nutrition Disorders; Body Weight; Overweight
• Other Study ID Numbers: 201912HM107-

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No