Influence of WNT4 VEZT FSHB and SIRT1 SNPs in Endometriosis: a Case Control Study of the Sardinian Population

February 12, 2021 updated by: Stefano Angioni, University of Cagliari

Influence of WNT4 VEZT FSHB and SIRT1 Genetic Polymorphisms in the Pathogenesis of Endometriosis: a Case Control Study of the Sardinian Population

Endometriosis, defined as the presence of endometrial tissue outside the uterine cavity, affects 6-10% of the general population of women in childbearing age.

The pathogenesis of the disease is unknown. The purpose of this study is to evaluate the influence of certain polymorphisms of genes WNT4, VEZT, FSHB, known to be involved in molecular mechanisms associated with phenomena of proliferation and development of endometriotic lesions, and SIRT1, that based on metabolomics studies, could hypothetically have a role in the pathogenesis of the disease. The study focus on the Sardinian population, known to have unique genetic characteristics due to geographical isolation.

Study Overview

Status

Unknown

Conditions

Intervention / Treatment

Detailed Description

Endometriosis is a benign estrogen-dependent disease characterized by the presence of endometrial tissue in its glandular and stromal components in locations other than the uterine cavity . Represents one of the most common gynecological, chronic inflammatory diseases of women of reproductive age, affecting approximately 10% of the female population, although this estimate may be lower than the real data considering the heterogeneity of the clinical manifestations of the disease and the tendency to therefore be underdiagnosed . Endometriosis is often associated with infertility and severe pain symptomatology including chronic pelvic pain, dyspareunia and dysmenorrhea. Despite numerous studies, the pathogenesis of the disease is still unknown. Studies on family aggregation and twins have given prominence to the genetic component, demonstrating how the genetic structure inherited from an individual influences 51% of the predisposition to develop endometriosis in life. A great deal of work has involved the identification of different gene polymorphisms able to help explain the susceptibility to the development of endometriosis, often with conflicting results . Genome-wide association studies (GWAS) and related meta-analyzes have led to the identification of disease risk loci by providing new insights into potential molecular pathways involved in endometriosis. At present, 19 independent single-nucleotide polymorphisms (SNPs) have been validly associated with endometriosis, explaining 5.19% of the variance of the disease.In particular, a recent study investigated three of these SNPs on the Greek population: rs7521902, rs10859871 and rs11031006, mapped respectively on the WNT4, VEZT and FSHB genes, highlighting a significant association with endometriosis. The WNT4 gene, located on chromosome 1, codes for a fundamental protein in the development of the female reproductive system . The expression of WNT4 has also been demonstrated at the level of the normal peritoneum suggesting a possible metaplastic hypothesis in promoting the transformation of peritoneal cells into endometriotic cells using pathways involved in the development of female genital tracts. Moreover WNT4 is equally expressed in the eutopic endometrium during the proliferative and secretory phases; variants of this gene could hypothetically contribute to abnormal growth of endometrial cells in ectopic sites. VEZT is a gene located on chromosome 12 and coding for vezatin, an important component of the cadherin-catenin complex which is essential for the formation and maintenance of adherent joints. The protein is expressed in most epithelial cells and is crucial for the formation of cell-cell contact junctions. The expression of VEZT is altered in the endometrium of patients with endometriosis and is an excellent candidate for having a causal role in endometriosis. In particular, the rs10859871 polymorphism was associated with an increase in the expression of this gene.The FSHB gene, located on chromosome 11, codes for the hormone-specific subunit b of the stimulating follicle hormone (FSH), a key promoter of ovarian follicle growth and estrogen production. The extended GWAS of the Sapkota group identifies a significant association between the rs11031006 polymorphism and the risk of endometriosis.

The metabolomic profile in patients affected by endometriosis have identified beta-hydroxybutyrate (bOHB) as a significantly increased metabolite in affected patients. The bOHB is a ketonic body that acts as an energy carrier from adipocytes to peripheral tissues. However, it plays a heterogeneous role in cellular signaling and regulation of gene expression. Several enzymes involved in the generation of ketone bodies from lipids are both acetylated and succinylated and target a group of enzymatic activity proteins that act as NAD-dependent deacylases: sirtuins. Sirtuins have recently been studied for their involvement in the field of female reproductive function. Among these, sirtuine 1, SIRT1, acts as histone-deacetylase and is implicated in innumerable phenomena such as the regulation of gene transcription, aging, stress resistance, apoptosis, energy efficiency. Furthermore, SIRT1 plays an important role in regulating the expression of inflammatory cytokines in endometriotic stromal cells. The SIRT1 gene, located on chromosome 10, is over-expressed in the eutopic endometrium of women with endometriosis and is probably involved in the pathogenesis of endometriosis. SIRT1 controls among other things the acetylation of a fork transcription factor involved in the pathway metabolic synthesis of ketone bodies, FOXA2, in turn involved in the phenomena of proliferation and migration of endometriotic cells. Some SSPs of the SIRT1 gene are associated with insults from oxidative stress capable of inducing an abnormal proliferation of endometrial cells (k endometrium).

The study aims to verify the possible association between the presence of certain polymorphisms of genes known to have a hypothetical role in the pathogenesis of endometriosis and the development of this disease in the population of Sardinian origin.

The study will be carried out by molecular typing of the following single substitution polymorphisms (SNPs): rs7521902, rs10859871, rs11031006, rs2273773, mapped respectively in the WNT4, VEZT, FSHB and SIRT1 genes.

In this work will be described the frequency of alleles, genotypes and haplotypes of these SNPs among Sardinian women and will be evaluated their impact on susceptibility to the development of endometriosis.

The molecular-biological analyzes of single-substitution polymorphisms will be carried out starting from whole blood, taken, subject to informed consent, to exclusively Sardinian patients.

The investigators have chosen to limit the study only to patients originating from Sardinia, taking advantage of the peculiarity of this land, which can be considered a genetic macro-isolate. The genetic analysis of complex traits is in fact simplified in isolated populations like the Sardinian one, in which inbreeding and "founding effect" reduce the genetic diversity of complex and polygenic diseases such as endometriosis which can, in this way, be studied more easily.The goal is to identify a genetic characterization that can be used for the non-invasive diagnosis of the disease

Study Type

Observational

Enrollment (Anticipated)

200

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Italy
    • Cagliari
      • Monserrato, Cagliari, Italy, 09042
        • Recruiting
        • University of Cagliari,Obstetrics and Gynecological Department,
        • Contact:
        • Sub-Investigator:
          • Danilo Deiana

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years and older (ADULT, OLDER_ADULT)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Female

Sampling Method

Non-Probability Sample

Study Population

Sardinian women with or without diagnosis of endometriosis

Description

Inclusion Criteria (cases):

  • endometriosis diagnosis (clinic, ultrasound or histological)
  • women of Sardinian origin

Inclusion Criteria (controls):

  • no signs of endometriosis
  • women of Sardinian origin

Exclusion Criteria (cases and controls):

  • women of non-Sardinian origin
  • patients unable to provide written informed consent or to follow the procedures provided by the protocol

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

Cohorts and Interventions

Group / Cohort
Intervention / Treatment
Endometriosis
Sardinian Women with diagnosis of endometriosis
Genetic: SNPs analysis with Sanger sequencing
Research of frequency of SNPs (WNT4, VEZT, FSHB and SIRT1 genes) in Sardinian women affected by endometriosis and controls
Controls
Healthy blood Sardinian donors
Genetic: SNPs analysis with Sanger sequencing
Research of frequency of SNPs (WNT4, VEZT, FSHB and SIRT1 genes) in Sardinian women affected by endometriosis and controls

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Time Frame
Frequency of Single Nucleotide Polymorphisms of WNT4, VEZT, FSHB and SIRT1 in Sardinian women with and without endometriosis diagnosis
Time Frame: baseline
baseline

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

University of Cagliari

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

September 1, 2019

Primary Completion (Anticipated)

June 1, 2021

Study Completion (Anticipated)

December 1, 2021

Study Registration Dates

First Submitted

March 9, 2015

First Submitted That Met QC Criteria

March 9, 2015

First Posted (Estimate)

March 17, 2015

Study Record Updates

Last Update Posted (Actual)

February 15, 2021

Last Update Submitted That Met QC Criteria

February 12, 2021

Last Verified

February 1, 2021

More Information

Terms related to this study

Additional Relevant MeSH Terms

Other Study ID Numbers

  • ENDOSNPs

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

Subscribe