Influenza Challenge Study in Healthy Volunteers

An Open Label Dose-Ranging Influenza Challenge Study in Healthy Volunteers

A study to determine the infectious titre of an Influenza A (H1N1) (A/New Caledonia/20/99) virus for use in other Influenza challenge studies for the development of Influenza vaccines, antiviral agents and to better understand the host response to Influenza infection.

Study Overview

• Status: Completed
• Conditions: Influenza
• Intervention / Treatment: Biological: High Titre Influenza virus; Biological: Medium - High Influenza virus; Biological: Medium Low Titre Influenza virus; Biological: Low titre

Detailed Description

The primary aim was to determine the infectious titre of an Influenza A (H1N1) (A/New Caledonia/20/99) virus for use in other Influenza challenge studies for the development of Influenza vaccines, antiviral agents and to better understand the host response to Influenza infection.

In addition, the safety of the inoculum, the virus shedding from the nasal mucosa following challenge with Influenza., changes in mRNA and protein expression from blood and nasal brush samples. were assessed Self reported symptoms following challenge were recorded. Samples were taken to facilitate validation of CMI and other immunologically relevant assays in development

Overall Trial Design:

For each group of subjects, quarantine, challenge phase, release from quarantine and decontamination occured concurrently at the designated study site throughout the challenge phase of the study.

The volunteers were be stratified into four groups depending on their intended challenge titre of virus:

High titre Medium-high titre Medium-low titre Low titre Influenza inoculation (nasal drops)

• Study Type: Interventional
• Enrollment (Actual): 24
• Phase: Phase 2; Phase 1

Contacts and Locations

Study Locations

• United Kingdom
  • London, United Kingdom, e1 2ax
    • hVIVO Services Ltd, QMB Bioenterprise building

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Age 18 to 45 years, inclusive.
• Comprehension of the study requirements;availability for the required study period, ability to attend scheduled study visits, and willingness to participate in the inpatient challenge.
• Willingness to provide written consent for participation after reading the Consent Form and after having adequate opportunity to discuss the study with an investigator or qualified deputy.
• Good general health status as determined by a screening evaluation no greater than 45 days prior to the quarantine and challenge phase.
• For female subjects, provision of a history of reliable contraceptive practices [hysterectomy or bilateral tubal ligation, oral or implanted contraceptive use, intrauterine device, barrier method plus spermicide, history of a single male partner with vasectomy, or a history of abstinence deemed credible by the investigator(s)]. The provision of this history does NOT replace the requirement to perform, and obtain negative results in, pregnancy tests.

Exclusion Criteria:

• Presence of any significant acute or chronic, uncontrolled medical or psychiatric illness,
• Health care workers (including doctors, nurses, medical students and allied healthcare professionals) anticipated to have patient contact within two weeks of viral challenge. Healthcare workers who volunteer should not work with patients until 14 days after challenge or until their symptoms are fully resolved.
• Venous access deemed inadequate for the phlebotomy demands of the study.
• Positive serologic tests for HIV, Hepatitis B surface antigen, or Hepatitis C antibody.
• Evidence of drug abuse or a positive urine Class A drug or alcohol screen.
• Female subjects, who are known to be pregnant or who have a positive urine β-HCG detected prior to challenge.
• Chronic use of any medication or other product (prescription or over-the-counter), for symptoms of rhinitis or nasal congestion or for any chronic nasopharyngeal complaint, or chronic use of any intranasal medication for any indication.
• Any history during adulthood of asthma of any etiology (chronic obstructive pulmonary disease), or any use of a bronchodilator or other asthma medication within adulthood.
• Smokers unwilling/unable to desist for the quarantine phase duration of the study.
• Subject is type I or type II diabetic.
• Subject is allergic to Gentamicin.
• Acute use of any medication or other product, prescription or over-the-counter, for symptoms of rhinitis or nasal congestion within seven (7) days prior to challenge. This includes any corticosteroid or beta agonist containing nasal spray.
• An Abnormal ECG deemed clinically relevant by the Investigator(s).
• Any anatomic or neurologic abnormality impairing the gag reflex or conducive to aspiration, or history suggestive of such a problem or any abnormality significantly altering the anatomy of the nose or nasopharynx.
• Receipt of systemic glucocorticoids (in a dose ≥5 mg prednisone daily or equivalent) within one month, or any other cytotoxic or immunosuppressive drug within six months prior to challenge.
• Receipt of any investigational drug within six (6) months prior to challenge, or prior participation in a clinical trial of any Influenza vaccine, or any investigational vaccine or experimental Influenza viral challenge delivered directly to the respiratory tract within 1 year prior to challenge.

• Presence of any febrile illness or symptoms of upper viral respiratory infection:

  1. existing on the day of challenge or between admission for Influenza challenge and administration of the challenge inoculum. (Such subjects may be re-evaluated for enrollment after resolution of the illness);
  2. within two (2) weeks prior to challenge or if challenge is set to occur during November, December, January, February, or March if there are any symptoms suggestive of viral respiratory infection occurring between screening and challenge.
• History of frequent epistaxis (nose bleeds).
• Presence of household member or close contact (for an additional two weeks after discharge from the isolation facility) who is: (a) less than 3 years of age; (b) any person with any known immunodeficiency; (c) any person receiving immunosuppressant medications; (d) any person undergoing or soon to undergo cancer chemotherapy within 28 days of challenge; (e) any person who has diagnosed emphysema or COPD, is elderly residing in a nursing home, or with severe lung disease or medical condition including but not exclusive to the conditions listed in Appendix 8; or (f) any person who has received a transplant (bone marrow or solid organ).
• Any laboratory test which is abnormal and which is deemed by the Investigator(s) to be clinically significant. (This includes blood chemistry, haematology, cardiac iso-enzymes, or urinalysis).
• Known IgA deficiency, immotile cilia syndrome, or Kartagener's syndrome.
• History of seasonal hay fever or a seasonal allergic rhinitis (SAR), including the use of symptomatic prescription only medication and non prescription medication.
• As a result of the medical interview, physical exam, or screening investigations, the Investigator(s) considers the subject unfit for the study.
• Those employed or immediate relatives of those employed at Retroscreen Virology or the study site.
• Receipt of a northern hemisphere seasonal influenza vaccine in the 2006/07/08 winter seasons.
• Receipt of any systemic chemotherapy agent at any time.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: High Titre Influenza virus	Biological: High Titre Influenza virus; High Titre Influenza virus
Experimental: Medium - High Influenza virus	Biological: Medium - High Influenza virus; Medium - High Influenza virus
Experimental: Medium Low Titre Influenza virus	Biological: Medium Low Titre Influenza virus; Medium Low Titre Influenza virus
Experimental: Low titre Influenza Vaccine	Biological: Low titre; Low titre

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
The infection rate of an Influenza (A/New Caledonia/20/99) virus. in human volunteers	14 days

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Self Reported Symptoms	Volunteers complete a standardized diary card, rating there symptoms from 0 to 3. The total score for each day is reported.	14 days
Virus Shedding from Nasal Mucosa	Nasal wash samples are taken daily and virus titre measured using tissue culture and reported in TCID50.	14 days

Collaborators and Investigators

• Sponsor: Hvivo
• Investigators: Principal Investigator: Robert Lambkin-Williams, PhD, 020 7756 1300

Study record dates

Study Major Dates

• Study Start: January 1, 2008
• Primary Completion (Actual): December 1, 2008
• Study Completion (Actual): December 1, 2008

Study Registration Dates

• First Submitted: February 23, 2016
• First Submitted That Met QC Criteria: April 18, 2016
• First Posted (Estimate): April 21, 2016

Study Record Updates

• Last Update Posted (Estimate): April 21, 2016
• Last Update Submitted That Met QC Criteria: April 18, 2016
• Last Verified: February 1, 2016

More Information

Terms related to this study

• Keywords: immunology; pathogenicity
• Additional Relevant MeSH Terms: RNA Virus Infections; Virus Diseases; Infections; Respiratory Tract Infections; Respiratory Tract Diseases; Orthomyxoviridae Infections; Influenza, Human
• Other Study ID Numbers: RIS-110 (DEE-CS-002)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: Only in anonymised form