Nutrient Supplementation Influences Mitochondria Adaptation

Effect of Whey Protein Hydrolysate on Adaptions to Endurance Training in Well-trained Runners

Twenty-four well trained runners (VO2max 60.73.7 ml O2 Kg-1 min1) completed a six week block randomized controlled intervention period. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO- CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 0.1g carbohydrate kg-1) after each exercise session. The CHO group ingested energy matched carbohydrate. Post-absorptive muscle biopsies were obtained pre and post intervention for analysis HAD, CS and PGC-1. Subjects completed two physical tests 6 km performance test (pre, week 1 and week 6) and aVo2max test (pre and post intervention).

Study Overview

• Status: Completed
• Conditions: Performance; Nutrition; Protein
• Intervention / Treatment: Dietary supplement: PROTEIN-CARBOHYDRATE; Dietary supplement: CARBOHDYRATE

• Study Type: Interventional
• Enrollment (Actual): 24
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Aarhus, Denmark, 8000
    • Aarhus University, Department for Public Health, Section for Sport Science
  • Aarhus, Denmark, 8000
    • Sport Science, Department of Public Health, Aarhus University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 55 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

1. maximal oxygen uptake (VO2max) of >50ml O2 kg-1 min-1 for women and >55ml O2 kg-1 min-1 for men,
2. running at least 3 times a week and
3. running being the primarily training form

Exclusion Criteria:

1. use of medicine
2. diagnosed metabolic diseases
3. injuries which hindered running and body mass index (BMI) < 25 kg m-2

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: PROTEIN-CARBOHYDRATE; Intervention group receiving protein and carbohydrate supplement	Dietary supplement: PROTEIN-CARBOHYDRATE; PRO before and PRO-CHO after training
Placebo Comparator: CARBOHYDRATE; Intervention groups receiving carbohydrate supplement	Dietary supplement: CARBOHDYRATE; CHO before and after training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Maximal oxygen uptake	maximal oxygen uptake (L O2/min)	Change from week 1 to week 6

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
VDAC	Mitochondria target measured by western blotting	Change from week 1 to week 6
CytC	Mitochondria enzyme measured by western blotting	Change from week 1 to week 6
Performance	6 km trial test	Change from week 1 to week 6
COX-IV	Mitochondria target measured by western blotting	Change from week 1 to week 6
HSP60	Mitochondria target measured by western blotting	Change from week 1 to week 6
CD36	Fat transporter measured by western blotting	Change from week 1 to week 6

Collaborators and Investigators

• Sponsor: University of Aarhus

Publications and helpful links

General Publications

• Hansen M, Oxfeldt M, Larsen AE, Thomsen LS, Rokkedal-Lausch T, Christensen B, Rittig N, De Paoli FV, Bangsbo J, Ortenblad N, Madsen K. Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners. J Int Soc Sports Nutr. 2020 Sep 7;17(1):46. doi: 10.1186/s12970-020-00376-3.

Study record dates

Study Major Dates

• Study Start (Actual): October 1, 2013
• Primary Completion (Actual): February 1, 2014
• Study Completion (Actual): June 1, 2014

Study Registration Dates

• First Submitted: December 1, 2017
• First Submitted That Met QC Criteria: June 7, 2018
• First Posted (Actual): June 19, 2018

Study Record Updates

• Last Update Posted (Actual): May 14, 2020
• Last Update Submitted That Met QC Criteria: May 12, 2020
• Last Verified: May 1, 2020

More Information

Terms related to this study

• Other Study ID Numbers: AURunners2017

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No