Is Adipose Tissue a Reservoir for SARS-Cov2 Spread in Covid-19 Patients? (TA/SARS-Cov2)

Is Adipose Tissue a Reservoir for SARS-Cov2 Spread?

Resident cells of human adipose tissue express ACE2 and DPP4, receptors for SARS-Cov2. The hypothesis is that the virus may enter and spread in fat depots.

Study Overview

• Status: Unknown
• Conditions: Covid-19 Patients
• Intervention / Treatment: Procedure: biopsies of subcutaneous adipose tissue

• Study Type: Interventional
• Enrollment (Anticipated): 5
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Vanina OLIVERI, PM; Phone Number: 0033 4 92 03 42 54; Email: oliveri.v@chu-nice.fr

Study Locations

• France
  • Nice, France, 06000
    • Chu de Nice
    • Contact: Vanina OLIVERI, PM; Email: oliveri.v@chu-nice.fr
    • Principal Investigator: Berengère Chignon-Sicard, PH

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Male or female ≥ 18 years old
• Patients testing positive for Covid19
• Patients affiliated to social security
• Signing of free and informed consent

Exclusion Criteria:

• Vulnerable persons (- minors,
• adults under guardianship or curatorship,
• pregnant women,
• persons deprived of their liberty,
• persons who do not master the French language)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: patients with Covid-19	Procedure: biopsies of subcutaneous adipose tissue; Two 4mm punch biopsies of subcutaneous abdominal or thight adipose tissue

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
The presence of SARS-CoV2 in adipose tissue of Covid19 patients	RT-qPCR for SARS-Cov-2 from RNAs and by immunodetection	Day 0

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
The expression of inflammatory cytokines in adipose tissue of Covid19 patients	RT-qPCR for IL6	Day 0
The expression of inflammatory cytokines in adipose tissue of Covid19 patients	RT-qPCR for IL1	Day 0
The expression of inflammatory cytokines in adipose tissue of Covid19 patients	RT-qPCR for TNFa	Day 0

Collaborators and Investigators

• Sponsor: Centre Hospitalier Universitaire de Nice

Study record dates

Study Major Dates

• Study Start (Anticipated): July 1, 2020
• Primary Completion (Anticipated): July 9, 2020
• Study Completion (Anticipated): July 9, 2021

Study Registration Dates

• First Submitted: June 10, 2020
• First Submitted That Met QC Criteria: June 11, 2020
• First Posted (Actual): June 11, 2020

Study Record Updates

• Last Update Posted (Actual): June 11, 2020
• Last Update Submitted That Met QC Criteria: June 11, 2020
• Last Verified: June 1, 2020

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Coronavirus Infections; Coronaviridae Infections; Nidovirales Infections; RNA Virus Infections; Virus Diseases; Infections; Respiratory Tract Infections; Respiratory Tract Diseases; Pneumonia, Viral; Pneumonia; Lung Diseases; COVID-19
• Other Study ID Numbers: 20-PP-15

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No