Malaria as a Risk Factor for COVID-19 in Western Kenya and Burkina Faso (MALCOV)

It is unknown whether malaria or malaria treatment affects COVID-19 severity, immune responses to SARS-CoV-2 virus, or viral loads and/or duration of shedding and therewith the onwards spread of SARS-COV-2. An observational cohort study will be conducted in 708 newly diagnosed COVID-19 patient of all ages in western Kenya and Burkina-Faso. They will be enrolled in hospitals with COVID-19 testing facilities from a source population screened for SARS-CoV-2 (N~4,720). Approximately 142 of the 708 COVID-19 patients are expected to be co-infected with malaria. They will be enrolled in the nested malaria treatment trial and randomized to receive 3-days of artemether-lumefantrine (the current standard of care) or pyronaridine-artesunate, a highly effective antimalarial with known antiviral properties against SARS-CoV-2 in-vitro, that is newly registered and being rolled out in Africa. Disease progression will be assessed and nasal swabs and blood samples will be taken during home/clinic visits on days 1, 3, 7, 14, 21, 28, and 42. Patients self-isolating will be phoned daily in between scheduled visits for the first 14 days to assess signs and symptoms. Hospitalisation, self-isolation and home-based care will follow national guidelines. The WHO clinical progression scale and FLU-PRO plus scales will be used to compare disease progression between COVID-19 patients with and without malaria, and by malaria. Other endpoints include seroconversion/reversion rates, chemokine/cytokine responses, T and B cell responses, viral load and duration of viral carriage. Infection prevention and control (IPC), including the use of personal protection equipment (PPE), and measures for patient transport will follow national guidelines in each country. Written informed consent/assent will be sought. The study is anticipated to start in January 2021 and last for approximately 18 months.

Study Overview

• Status: Completed
• Conditions: Covid-19; Malaria
• Intervention / Treatment: Drug: Artemether-lumefantrine (AL); Drug: Pyronaridine-artesunate (PA)

Detailed Description

Background: In Africa, COVID-19 has the potential to cripple the continent's fragile healthcare systems and be devastating economically. It is unknown whether malaria infection worsens COVID-19, affects the acquisition of protective antibodies against the SARS-CoV-2 virus, or contributes to its onwards spread by resulting in higher viral loads and/or longer duration of viral shedding. It is also unknown if the effective clearance of malaria parasites and/or the choice of antimalarials affects any of these potential associations. His study will determine if the antimalarial pyronaridine, in the fixed-dose combination of pyronaridine-artesunate, has a positive, negative or negligible effect on COVID-19 disease progression or duration of viral carriage and the seroconversion rate to SARS-CoV-2.

Methods: A malaria treatment trial will be conducted nested within a larger observational COVID-19 cohort study in highly malaria-endemic areas in western Kenya and Burkina-Faso. The COVID-19 cohort study consists of approximately 708 newly diagnosed COVID-19 patient of all ages. They will be enrolled from a source population of approximately 4,720 individuals of all ages screened for SARS-CoV-2. It is anticipated that approximately 142 of the 708 cohort participants will be co-infected with malaria. These co-infected participants will be enrolled in the nested malaria treatment trial if they have uncomplicated malaria and are able to take oral medication. They will be randomized to receive either a standard 3-day treatment course of artemether-lumefantrine (the current first-line treatment) or pyronaridine-artesunate, a new highly effective antimalarial combination that is being rolled out as first or second-line treatment in western Kenya and Burkina Faso. All 142 patients will be followed for 42 days and nasal swabs and blood samples taken on days 1, 3, 7, 14, and 28. Malaria smears will be taken on days 3, 7, 14, 21, 28 and 42. The primary endpoint is the rate of SARS-CoV-2 clearance by day-7.

To limit the transmission of SARS-CoV-2, strict adherence to infection prevention and control (IPC) guidelines, including use of personal protection equipment (PPE), and measures for patient transport will be followed as per national guidelines in each country. Written informed consent/assent will be sought.

Partners: This 18-months study is funded by the Bill and Melinda Gates Foundation and is part of a collaboration between the Kenyan Medical Research Institute (KEMRI) in Kenya; the US Centers for Disease Control and Prevention (CDC); the Liverpool School of Tropical Medicine (LSTM); the Ministry of Health, Kenya; the Groupe de Recherche Action en Santé (GRAS), Ouagadougou, Burkina Faso; the Ministry of Health in Burkina Faso, and the London School of Hygiene and Tropical Medicine (LSHTM). LSTM and LSHTM will act as sponsors for the studies in Kenya and Burkina Faso, respectively.

• Study Type: Interventional
• Enrollment (Actual): 143
• Phase: Phase 3

Contacts and Locations

Study Locations

• Burkina Faso
  • Ouagadougou, Burkina Faso, 06BP10248
    • Ouagadougou Hospitals
• Kenya
  • Kisumu, Kenya, 40100
    • Kisumu County Referral Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 6 months to 100 years (Child, Adult, Older Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Laboratory confirmed SARS-CoV-2 infection, with positive molecular test results within the past 72 hours*
• Aged >=6 months **
• Resident in the study area
• The participant or caretaker is willing and able to give informed consent or assent with parent/guardian informed consent for participation in the study
• Agrees not to self-medicate with chloroquine, hydroxychloroquine or other antimalarials with potential anti-SARS-CoV-2 properties
• Not previously diagnosed with COVID-19
• Contactable by phone for follow-up permitting real-time, reliable information
• Uncomplicated malaria, defined as able to take oral medication
• Bodyweight ≥5kg
• Confirmed malaria infection by RDT (pLDH) or microscopy

Exclusion Criteria:

• Unwilling or unable to provide informed consent/assent
• The participant is judged by the Investigator to be at significant risk of failing to comply with the provisions of the protocol as to cause harm to self or seriously interfere with the validity of the study results
• Inability/unlikely to be in the study area for the duration of the 28-day follow-up period
• Pregnant or lactating women
• Severe disease requiring parenteral treatment
• Currently receiving, or recently received (within the last 28 days) pyronaridine-artesunate or artemether-lumefantrine
• Received chloroquine in the last three days
• Inability/unlikely to be in the study area for the duration of the 42-day follow-up period
• Known hypersensitivity or specific contraindication to the use of any of the study drugs in the treatment arms
• Known chronic kidney disease (signs or symptoms of stage IV renal impairment or receiving dialysis)
• Known liver cirrhosis (Child-Pugh Class B or greater) or signs or symptoms of severe hepatotoxicity

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Artemether-lumefantrine; Artemether-lumefantrine, standard 3-day antimalarial treatment regimen.	Drug: Artemether-lumefantrine (AL); Current first line treatment of malaria. Dose: Bodyweight (kg) Dose (mg) of artemether + lumefantrine given twice daily for 3 days (total, six doses) 5 to < 15 20 + 120 15 to < 25 40 + 240 25 to < 35 60 + 360 >=35 80 + 480; Twice daily for 3 days (total, six doses); Other Names: Coartem
Experimental: Pyronaridine-artesunate; Pyronaridine-artesunate, standard 3-day antimalarial treatment regimen.	Drug: Pyronaridine-artesunate (PA); Antimalarial; Dose: Body weight (kg) Dose (mg) of pyronaridine + aresunate given once daily for 3 days (total, three doses) 5 to < 8 60 + 20 8 to <15 120 + 40 15 to <20 180 + 60 20 to <24 kg 180 + 60 24 to <45 360 + 120 45 to <65 540 + 180 >=65 720 + 240; Once-daily for 3 days (total, three doses).; Other Names: Pyramax

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Incidence of SARS-CoV-2 clearance	Defined as the proportion of participants with a negative nasal swab on Day 7 after the start of treatment	by day 7

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Median viral load of SARS-CoV-2	Median CT value as detected from mid-nasal swabs by PCR	by day 14
Cumulative incidence of SARS-CoV-2 clearance	Defined as the proportion of participants with negative nasal swabs	by days 14, 21 and 28
Time to clearance of nasal SARS-CoV-2	Defined as the number of days to a negative SARS-CoV-2 RNA PCR tests (swabs collected on days 1, 3, 7, 14, 21 and 28)	by days 1, 3, 7, 14 and 28
Cumulative seroconversion rates (IgG, IgM, IgA)	proportion of antibody negative patients on enrolment who seroconvert	by days 7, 14, 21 and 28
IgG, IgM, IgA antibody titres against SARS-CoV-2	Geometric mean, maximum, and change from baseline	by days 7, 14, 21 and 28
IL-6, IL-7, IL-10, TNF-alpha and Interferon-Gamma	median, max and change from baseline	by days 3, 7, 14 and 28
CRP and angiotensin-2, angiopoietin-1 and angiopoietin-2	median, max and change from baseline	by days 3, 7, 14 and 28
Genomic responses to SARS-CoV-2 infection	Transcriptional profiling (gene expression) of whole blood and fixed whole blood for T and B cell markers	by day 28
Cellular immune responses to SARS-CoV-2 infection	T cell responses	by day 28
The clinical and parasitological antimalarial treatment response	Expressed as the proportion with early treatment failure, late clinical failure, late parasitological failure or an adequate clinical and parasitological response. Recrudescence will be differentiated from new infection by genotyping of malaria parasites	by day 42
COVID-19 disease severity	Defined by a severity index score for COVID-19	by day 28
COVID-19 disease duration	The proportion of days with symptoms after randomization	by day 28
COVID-19 fever duration	The proportion of days with a fever after randomization	by day 28
COVID-19 respiratory symptoms duration	The proportion of days with respiratory symptoms after randomization	by day 28
COVID-19 disease duration in days	The number of days until symptom clearance	by day 28
Treatment-related adverse events, serious adverse events, and adverse events resulting in treatment discontinuation	The cumulative proportion of patients with any of these events after the start of treatment	by day 7

Collaborators and Investigators

• Sponsor: Liverpool School of Tropical Medicine
• Collaborators: London School of Hygiene and Tropical Medicine; Bill and Melinda Gates Foundation; Centers for Disease Control and Prevention; Kenya Medical Research Institute; Groupe de Recherche Action en Sante; Centres for Disease Control and Prevention, Kenya.
• Investigators: Principal Investigator: Kariuki Simon, PhD, Kenya Medical Research Institute; Principal Investigator: Sirima Sodiomon, MD, PhD, Groupe de Recherche Action en Santé(GRAS)

Study record dates

Study Major Dates

• Study Start (Actual): January 8, 2021
• Primary Completion (Actual): November 1, 2022
• Study Completion (Actual): February 20, 2024

Study Registration Dates

• First Submitted: December 14, 2020
• First Submitted That Met QC Criteria: January 4, 2021
• First Posted (Actual): January 5, 2021

Study Record Updates

• Last Update Posted (Actual): February 23, 2024
• Last Update Submitted That Met QC Criteria: February 22, 2024
• Last Verified: February 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Coronavirus Infections; Coronaviridae Infections; Nidovirales Infections; RNA Virus Infections; Virus Diseases; Infections; Respiratory Tract Infections; Respiratory Tract Diseases; Pneumonia, Viral; Pneumonia; Lung Diseases; Vector Borne Diseases; Parasitic Diseases; Protozoan Infections; COVID-19; Malaria; Anti-Infective Agents; Antiviral Agents; Antineoplastic Agents; Antiprotozoal Agents; Antiparasitic Agents; Antimalarials; Anthelmintics; Schistosomicides; Antiplatyhelmintic Agents; Lumefantrine; Artemether; Artesunate; Artemether, Lumefantrine Drug Combination; Pyronaridine; Pyronaridine tetraphosphate, artesunate drug combination
• Other Study ID Numbers: 20-063; 202009642148520 (Registry Identifier: Pan African Clinical Trial Registry (PACTR))

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: We will encourage data sharing to ensure that the scientific potential of this study is maximized. The full anonymized research database will be made publicly available as soon as the full study findings have been published or based on any data requests that may occur during the study or analysis is still ongoing. For the databases, we will use a controlled access approach.
• IPD Sharing Time Frame: As soon as the full study findings have been published or based on any data requests that may occur during the study or analysis is still ongoing.
• IPD Sharing Access Criteria: Data access will be provided to researchers after a proposal has been approved by an independent review committee identified for this purpose. An agreement on how to collaborate will be reached based on any overlap between the proposal and any ongoing efforts. Proposals can be directed to email addresses provided in the publications and websites. To gain access, data requesters will need to sign a data-sharing agreement. The only limits to data sharing will be to safeguard research participants' confidentiality. External users will be bound by data-sharing agreements in line with the Data Sharing Policy from the respective Sponsors and the Gates Foundation to ensure that the privacy of individuals is protected. The agreement will prohibit any attempt to (a) identify study participants from the data or otherwise breach confidentiality, (b) make unapproved contact with study participants.
• IPD Sharing Supporting Information Type: STUDY_PROTOCOL

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No