The Effect of Different Methods for Modulating Postprandial Fat Oxidation on Postprandial Lipemia the Next Day

Time-restricted Feeding Increases Fat Oxidation Rate But Not Affect Postprandial Lipemia: a Crossover Trial

Background: Studies have revealed that time-restricted feeding affects the fat oxidation rate; however, its effects on the fat oxidation rate and hyperlipidemia following high-fat meals are unclear. This study investigated the effects of 5-day time-restricted feeding on the fat oxidation rate and postprandial lipemia following high fat meals. Methods: In this random crossover experimental study, eight healthy male adults were included each in the 5-day time-restricted feeding trial and the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00. The meals of the control trial were provided at 08:00, 14:00, and 20:00. The contents of the meals of both trials were the same, and the calories of the meals met the 24-hour energy requirement of the participants. After 5 days of the intervention, the participants consumed high-fat meals on the sixth day, and their physiological changes were determined.

Study Overview

• Status: Completed
• Conditions: Postprandial Lipemia; Time-restricted Feeding
• Intervention / Treatment: Other: time-restricted feeding trial

Detailed Description

The experiment was conducted over 6 days. On the first day, the participants arrived at the laboratory at 08:00 and quietly rested for 20 minutes in the supine position; gas analyzers were used to record their energy consumption. Subsequently, the participants were randomly allocated to the time-restricted feeding trial or the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00, and the participants were required to consume all the food during this time. The meals of the control trial were provided at 08:00, 14:00, and 20:00, but the consumption time was not limited. In addition to regular meals, a snack with approximately 200 calories was provided to the participants for consumption. The participants in the time-restricted feeding trial were only allowed to consume the snack from 12:00 to 20:00, whereas no restrictions were imposed on the control trial for snack consumption. The meals of the participants were provided by dieticians. Based on the results of the pretest, the calories of each meal met the daily energy requirement of the participants. The macronutrient consumption for TRF and CON were listed in table 1.

After experiment completion on the fifth day, the participants returned to the laboratory on the eighth day from 08:00 to 09:00. They rested for 10 minutes in the supine position, and gas analyzers were used to collect the gas data of the participants for 20 minutes. Next, a catheter was inserted into the forearm of each participant to collect fasting blood samples. After blood sample collection, the participants were provided with a specific high-fat meal. The participants rested quietly in the laboratory for 4 hours, and their blood lipid changes during this period were observed.

• Study Type: Interventional
• Enrollment (Actual): 8
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Taiwan
  • Taichung, Taiwan, 404
    • National Taiwan University of Sport

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• All the participants had not undergone physical training;
• they did not exercise regularly;
• and they did not have any diseases that would prevent them from performing exercises, such as high blood pressure, hyperlipidemia, heart disease, joint disease, and osteoporosis.

Exclusion Criteria:

• Undergone physical training;
• Exercise regularly;
• Have any diseases that would prevent them from performing exercises, such as high blood pressure, hyperlipidemia, heart disease, joint disease, and osteoporosis.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: time-restricted feeding trial; The participants were divided into the time-restricted feeding trial for 5 days	Other: time-restricted feeding trial; The 5-day time-restricted feeding trial and the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00.
Experimental: control trial; The participants in the control trial did not practice intermittent fasting methods	Other: time-restricted feeding trial; The 5-day time-restricted feeding trial and the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
postprandial triglycerides concentrations	postprandial triglycerides concentrations (mmol/L)	on the 6th day
postprandial glucose concentrations	postprandial glucose concentrations (mmol/L)	on the 6th day
postprandial insulin concentrations	postprandial insulin concentrations (pmol/L)	on the 6th day
postprandial free fatty acid concentrations	postprandial FFA concentrations (mmol/L)	on the 6th day
postprandial glycerol concentrations	postprandial glycerol concentrations (umol/L)	on the 6th day

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
fat oxidation	fasting fat oxidation rate (g/min-1)	on the 6th day

Collaborators and Investigators

• Sponsor: National Taiwan Sport University
• Investigators: Study Chair: ChihHui Chiu, PhD, National Taiwan University of Sport

Study record dates

Study Major Dates

• Study Start (Actual): June 4, 2021
• Primary Completion (Actual): February 3, 2022
• Study Completion (Actual): February 3, 2022

Study Registration Dates

• First Submitted: February 2, 2022
• First Submitted That Met QC Criteria: February 17, 2022
• First Posted (Actual): February 22, 2022

Study Record Updates

• Last Update Posted (Actual): February 22, 2022
• Last Update Submitted That Met QC Criteria: February 17, 2022
• Last Verified: February 1, 2022

More Information

Terms related to this study

• Keywords: intermittent fasting
• Additional Relevant MeSH Terms: Metabolic Diseases; Lipid Metabolism Disorders; Dyslipidemias; Hyperlipidemias
• Other Study ID Numbers: 110-10

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No