Gut-muscle Axis: Exploring the Gut Microbiota Influences Host Energy Sources and Exercise Performance

Gut-muscle Axis: Exploring the Gut Microbiota Influences Host Energy Sources and Exercise Performance Via Modulation of Lactate Metabolism and Utilization

Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science.

This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.

Study Overview

• Status: Not yet recruiting
• Conditions: Marathon Runner
• Intervention / Treatment: Device: running

Detailed Description

The gut microbiota, which is comprised of trillions of microbes, is the collective term for the gut microbiota. It establishes a symbiotic relationship with the host and plays a vital role in maintaining host health. Through training, the investigators can adjust the composition and functions of gut microbiota. Exercise also alters the body's energy metabolism pathways, which, in turn, impacts the gut microbiota and its metabolic products. Understanding how the body utilizes and regulates energy during exercise is essential for enhancing athletic performance. This study aims to explore the relationships among post-exercise energy metabolites, gut microbiota, and their metabolic products, applying omics technologies in the field of sports science.

This interdisciplinary research will contribute to fields such as exercise physiology, sports nutrition, exercise biochemistry, and sports medicine, providing innovative and practical research insights for advancing sports science in our country.

• Study Type: Interventional
• Enrollment (Estimated): 90
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Yi-Ju Hsu; Phone Number: +886-3-328-3201 #2430; Email: ruby780202@ntsu.edu.tw

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Marathon Running Group

Inclusion Criteria:

• Training frequency of at least 3 times per week in the past year, with each session involving at least 1 hour of running.
• Participation in at least 2 marathon events within the past 2 years, with a completion time of under 4 hours and 30 minutes for a full marathon, or under 2 hours and 15 minutes for a half marathon.

Exclusion Criteria:

• Excludes individuals with cardiovascular diseases, hypertension, epilepsy, bronchitis, severe asthma, heart disease, inflammatory bowel disease, bacterial or viral infections, disabilities, or chronic bone diseases.
• Habitual smokers.
• Individuals with highly irregular eating habits or sleep patterns due to shift work or night work.
• History of chronic neuro-muscular injuries or disorders in the past 6 months.
• Individuals who have received antibiotic treatment or taken probiotic supplements in the past 6 months.

General Healthy Adult Group

Inclusion Criteria:

• Generally healthy, untrained individuals.

Exclusion Criteria:

• Excludes individuals with cardiovascular diseases, hypertension, epilepsy, bronchitis, severe asthma, heart disease, inflammatory bowel disease, bacterial or viral infections, disabilities, or chronic bone diseases.
• Habitual smokers.
• Individuals with highly irregular eating habits or sleep patterns due to shift work or night work.
• History of chronic neuro-muscular injuries or disorders in the past 6 months.
• Individuals who have received antibiotic treatment or taken probiotic supplements in the past 6 months.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Marathon running group; Run 10 kilometers on a treadmill.	Device: running; Running 10 kilometers on a treadmill
No Intervention: General healthy adult group; No intervention will be conducted.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Concentration of lactate	Measure changes in lactate concentration.	The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, immediately after exercise, and at 5, 15, 30, and 60 minutes post-exercise.
Concentration of creatine kinase	Measure changes in creatine kinase concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of glucose	Measure changes in glucose concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of creatinine	Measure changes in creatinine concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of blood urea nitrogen	Measure changes in blood urea nitrogen concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of lactate dehydrogenase	Measure changes in lactate dehydrogenase concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of free fatty acid	Measure changes in free fatty acid concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
Concentration of cortisol	Measure changes in cortisol concentration.	The testing time points are set as follows: before exercise, immediately after exercise, and 60 minutes post-exercise.
ood frequency questionnaire(FFQ)	The participants' diets were assessed using a semiquantitative food frequency questionnaire(FFQ). The FFQ used in this study was validated in a previous study; the questionnaire exhibited reliability and validity for identifying major nutrients in the diets of Taiwanese vegetarians and omnivores.(Chiu TH et al., 2014) In this study, the nutrient analysis programme used to calculate the results of the FFQ was based on Taiwan's Food Composition Database. To assess the intake frequency of each food item, frequencies ranged from "never" or "<1 time per month" to "more than 2 times per day.	Before exercise
Gut microbiota analysis	Collect participant stool samples for gut microbiota analysis.	The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.
Untargeted metabolome analysis	Collect participant stool samples for metabolome analysis.Metabolite identification aims to analyze the metabolome composition of biological samples, investigate the regulation of metabolic pathways, and explore biochemical mechanisms.	The testing time points are set as follows: before exercise, immediately after exercise, and at 24 and 48 hours post-exercise.

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Body composition	The investigators used the InBody 570 (In-body, Seoul, South Korea), the bioelectrical impedance analyzer (BIA) to measure body composition. The device can screen frequencies of 1, 5, 50, 260, 500 and 1000 kHz within 60 seconds. After cleaning the palms and soles of the feet, the subject stood vertically on the electrodes of the instrument, holding the sensing handle with both hands and keeping the arms away from the body at a 30° angle, and avoided talking or moving during the measurement. The device measured body weight (kg), BMI (kg/m²), fat mass (%), and lean mass (kg).	Before exercise
Rate of Perceived Exertion(RPE)	Assess perceived exercise intensity.	The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise.
Heart rate	Assess changes in heart rate.	The testing time points are set as follows: before exercise, at the 5-kilometer mark during exercise, and immediately after exercise.

Collaborators and Investigators

• Sponsor: National Taiwan Sport University
• Investigators: Principal Investigator: Yi-Ju Hsu, Associate Professor

Study record dates

Study Major Dates

• Study Start (Estimated): November 8, 2024
• Primary Completion (Estimated): December 31, 2024
• Study Completion (Estimated): December 31, 2024

Study Registration Dates

• First Submitted: October 26, 2024
• First Submitted That Met QC Criteria: October 31, 2024
• First Posted (Estimated): November 1, 2024

Study Record Updates

• Last Update Posted (Estimated): November 1, 2024
• Last Update Submitted That Met QC Criteria: October 31, 2024
• Last Verified: October 1, 2024

More Information

Terms related to this study

• Other Study ID Numbers: NTSUIRB - 112 - 007

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No