The difference of T cell phenotypes in end stage renal disease patients under different dialysis modality

Jiao Xiaoyan, Chen Rongyi, Cao Xuesen, Zou Jianzhou, Ji Jun, Ding Xiaoqiang, Yu Xiaofang, Jiao Xiaoyan, Chen Rongyi, Cao Xuesen, Zou Jianzhou, Ji Jun, Ding Xiaoqiang, Yu Xiaofang

Abstract

Background: Impaired T cell immune function exists in end-stage renal disease (ESRD) patients. Dialysis treatment may lead to changes in T cell subsets. In the present study, we aimed to identify alterations of T cell phenotypes in ESRD patients, especially in those receiving peritoneal dialysis (PD), and analyze the potential associated factors.

Methods: In the present study, 110 PD patients and 110 age/gender-matched hemodialysis (HD) patients who met the inclusion criteria were studied. Pre-dialysis blood samples were obtained and analyzed by flow cytometry to detect the expression of CD45RO and CCR7. Univariate and multivariate regression analyses were used to determine the factors associated with the alteration of T cell phenotypes.

Results: In all dialysis patients, age was associated with the frequencies of both CD4+ and CD8+ naïve T cells, effector memory (EM) T cells and effector memory RA (EMRA) T cells but not central memory (CM) T cells. Dialysis modality was also associated with T cell subsets. Compared with HD patients, PD patients showed an increase in both CD4+ and CD8+ CM T cells and a reduction in both CD4+ and CD8+ EM and EMRA T cells. However, the number of CD4+ naïve T cells was lower and the number of CD8+ naïve T cells was higher in PD patients than those in HD patients. In PD patients, further multivariate analysis revealed that the frequency of CD4+ naïve T cells was positively associated with nPCR, while the frequency of CD8+ naïve T cells was negatively associated with age.

Conclusion: In dialysis patients, the dialysis modality and age influence T cell subsets. There is a progression from naïve to effector T cells in HD patients compared with PD patients. In PD patients, different factors may influence the frequencies of CD4+ and CD8+ naïve T cells.

Keywords: End-stage renal disease; Hemodialysis; Peritoneal dialysis; T cell phenotypes.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart of flow cytometry analysis to identify CD4 and CD8 T cells. a The flow cytometry picture of lysed peripheral blood including lymphocytes; b the flow cytometry picture of T cells gated from lymphocytes; c the flow cytometry picture of CD4 T cells and CD8 T cells gated from T cells; d the distribution of naïve T cells (CD45RO − CCR7+), central memory T cells (CM, CD45RO+ CCR7+), effector memory T cells (EM, CD45RO + CCR7−), EMRA (CD45RO − CCR7−) gated from CD4 T cells; e the distribution of naïve T cells (CD45RO − CCR7+), central memory T cells (CM, CD45RO+ CCR7+), effector memory T cells (EM, CD45RO + CCR7−), EMRA (CD45RO − CCR7−) gated from CD8 T cells

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