Validation of a 40-gene expression profile test to predict metastatic risk in localized high-risk cutaneous squamous cell carcinoma
Ashley Wysong, Jason G Newman, Kyle R Covington, Sarah J Kurley, Sherrif F Ibrahim, Aaron S Farberg, Anna Bar, Nathan J Cleaver, Ally-Khan Somani, David Panther, David G Brodland, John Zitelli, Jennifer Toyohara, Ian A Maher, Yang Xia, Kristin Bibee, Robert Griego, Darrell S Rigel, Kristen Meldi Plasseraud, Sarah Estrada, Lauren Meldi Sholl, Clare Johnson, Robert W Cook, Chrysalyne D Schmults, Sarah T Arron, Ashley Wysong, Jason G Newman, Kyle R Covington, Sarah J Kurley, Sherrif F Ibrahim, Aaron S Farberg, Anna Bar, Nathan J Cleaver, Ally-Khan Somani, David Panther, David G Brodland, John Zitelli, Jennifer Toyohara, Ian A Maher, Yang Xia, Kristin Bibee, Robert Griego, Darrell S Rigel, Kristen Meldi Plasseraud, Sarah Estrada, Lauren Meldi Sholl, Clare Johnson, Robert W Cook, Chrysalyne D Schmults, Sarah T Arron
Abstract
Background: Current staging systems for cutaneous squamous cell carcinoma (cSCC) have limited positive predictive value for identifying patients who will experience metastasis.
Objective: To develop and validate a gene expression profile (GEP) test for predicting risk for metastasis in localized, high-risk cSCC with the goal of improving risk-directed patient management.
Methods: Archival formalin-fixed paraffin-embedded primary cSCC tissue and clinicopathologic data (n = 586) were collected from 23 independent centers in a prospectively designed study. A GEP signature was developed using a discovery cohort (n = 202) and validated in a separate, nonoverlapping, independent cohort (n = 324).
Results: A prognostic 40-GEP test was developed and validated, stratifying patients with high-risk cSCC into classes based on metastasis risk: class 1 (low risk), class 2A (high risk), and class 2B (highest risk). For the validation cohort, 3-year metastasis-free survival rates were 91.4%, 80.6%, and 44.0%, respectively. A positive predictive value of 60% was achieved for the highest-risk group (class 2B), an improvement over staging systems, and negative predictive value, sensitivity, and specificity were comparable to staging systems.
Limitations: Potential understaging of cases could affect metastasis rate accuracy.
Conclusion: The 40-GEP test is an independent predictor of metastatic risk that can complement current staging systems for patients with high-risk cSCC.
Keywords: cutaneous squamous cell carcinoma; gene expression profile; metastasis; prognostication; risk.
Copyright © 2020 American Academy of Dermatology, Inc. All rights reserved.
Source: PubMed