Clinical microfluidics for neutrophil genomics and proteomics
Kenneth T Kotz, Wenzong Xiao, Carol Miller-Graziano, Wei-Jun Qian, Aman Russom, Elizabeth A Warner, Lyle L Moldawer, Asit De, Paul E Bankey, Brianne O Petritis, David G Camp 2nd, Alan E Rosenbach, Jeremy Goverman, Shawn P Fagan, Bernard H Brownstein, Daniel Irimia, Weihong Xu, Julie Wilhelmy, Michael N Mindrinos, Richard D Smith, Ronald W Davis, Ronald G Tompkins, Mehmet Toner, Inflammation and the Host Response to Injury Collaborative Research Program, Henry V Baker, Ulysses G J Balis, Timothy R Billiar, Steven E Calvano, J Perren Cobb, Joseph Cuschieri, Celeste C Finnerty, Richard L Gamelli, Nicole S Gibran, Brian G Harbrecth, Douglas L Hayden, Laura Hennessy, David N Herndon, Marc G Jeschke, Jeffrey L Johnson, Matthew B Klein, Stephen F Lowry, Ronald V Maier, Philop H Mason, Grace P McDonald-Smith, Joseph P Minei, Ernest E Moore, Avery B Nathens, Grant E O'Keefe, Laurence G Rahme, Daniel G Remick, David A Schoenfeld, Michael B Shapiro, Jason Sperry, John D Storey, Robert Tibshirani, H Shaw Warren, Michael A West, Bram Wispelwey, Wing H Wong, Kenneth T Kotz, Wenzong Xiao, Carol Miller-Graziano, Wei-Jun Qian, Aman Russom, Elizabeth A Warner, Lyle L Moldawer, Asit De, Paul E Bankey, Brianne O Petritis, David G Camp 2nd, Alan E Rosenbach, Jeremy Goverman, Shawn P Fagan, Bernard H Brownstein, Daniel Irimia, Weihong Xu, Julie Wilhelmy, Michael N Mindrinos, Richard D Smith, Ronald W Davis, Ronald G Tompkins, Mehmet Toner, Inflammation and the Host Response to Injury Collaborative Research Program, Henry V Baker, Ulysses G J Balis, Timothy R Billiar, Steven E Calvano, J Perren Cobb, Joseph Cuschieri, Celeste C Finnerty, Richard L Gamelli, Nicole S Gibran, Brian G Harbrecth, Douglas L Hayden, Laura Hennessy, David N Herndon, Marc G Jeschke, Jeffrey L Johnson, Matthew B Klein, Stephen F Lowry, Ronald V Maier, Philop H Mason, Grace P McDonald-Smith, Joseph P Minei, Ernest E Moore, Avery B Nathens, Grant E O'Keefe, Laurence G Rahme, Daniel G Remick, David A Schoenfeld, Michael B Shapiro, Jason Sperry, John D Storey, Robert Tibshirani, H Shaw Warren, Michael A West, Bram Wispelwey, Wing H Wong
Abstract
Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.
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Source: PubMed