Molecular dysfunctions in acute rejection after renal transplantation revealed by integrated analysis of transcription factor, microRNA and long noncoding RNA
Weiguo Sui, Hua Lin, Wujian Peng, Yuanshuai Huang, Jiejing Chen, Yue Zhang, Yong Dai, Weiguo Sui, Hua Lin, Wujian Peng, Yuanshuai Huang, Jiejing Chen, Yue Zhang, Yong Dai
Abstract
Acute rejection remains a problem in renal transplantation. To further illustrate the mechanism of rejection, we integrated protein array-based proteomics and RNA microarray-based genomics to investigate the transcription factor, microRNA and long noncoding RNA of biopsies of three patients with acute rejections and a control group. 99 transcription factors were identified in acute rejection biopsies compared to normal renal tissue. We correlated transcription factor data with microRNA and long noncoding RNA data sets and reported the expression of 5 transcription factors (AP-1, AP-4, STATx, c-Myc and p53), 12 miRNAs and 32 lncRNAs in acute rejection biopsies. Pathway analysis demonstrated that over-presentation of transcription factor pathway plays a critical role in acute rejection. This is the first study to comprehensively report the acute rejection transcription factor pathway. Integrative analysis of the transcription factor, microRNA and long noncoding RNA provided an expansive view of molecular signaling pathways in acute rejection after renal transplantation.
Keywords: AR; Kidney transplantation; Long noncoding RNA, linc-MD1, human; MicroRNAs; Microarray analysis; Protein microarrays; QRT-PCR; SCOR; Stem Cell Omics Repository; TFs; Transcription factors; acute rejection; lncRNA; long noncoding RNA; miRNA; microRNA; quantitative real-time PCR; transcription factors.
Copyright © 2013 Elsevier Inc. All rights reserved.
Source: PubMed