Design and Analysis of Single-Cell Sequencing Experiments
Dominic Grün, Alexander van Oudenaarden, Dominic Grün, Alexander van Oudenaarden
Abstract
Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.
Copyright © 2015 Elsevier Inc. All rights reserved.
Source: PubMed