Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation

Miguel Alcaraz, Samuel Quesada, David Armero, Rocio Martin-Gíl, Amparo Olivares, G Daniel Achel, Miguel Alcaraz, Samuel Quesada, David Armero, Rocio Martin-Gíl, Amparo Olivares, G Daniel Achel

Abstract

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001). However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

Keywords: Anaesthesia; genotoxicity; micronucleus; radiation effects; sevofluorane.

Conflict of interest statement

Conflict of interest: The authors declare no conflict of interest.

Figures

Figure 1.. a) Effect of different volumes…
Figure 1.. a) Effect of different volumes of sevofluorane on PNT2 cell viability. b) Radiation dose effects on PNT2 cell viability after 24 and 48 h incubation. Results are expressed as a percentage of surviving PNT2 cells in the control (RO: rosmarinic acid 25 μM irradiated and 48 h incubation; DMSO: Dimetyl sulphoxide 0.2% irradiated and incubated 48h) (1) p <0.001 versus control, (2) p <0.001 versus irradiated control.
Figure 2.. Genotoxic effect (Frequency of MN/500CB)…
Figure 2.. Genotoxic effect (Frequency of MN/500CB) of different volumes of sevofluorane and radiation: C control, RO Rosmarinic acid, DMSO Dimethyl sulfoxide and S5, S20, S40 different volumes of sevofluorane (µL) administered alone or before X-irradiation ((1) p <0.001 versus non-irradiated control, (2) p <0.001 versus irradiated control, (3) p< 0.01 versus irradiated control, (4) p <0.001 versus S5 non-irradiated.

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