COVID-19: Nasal and oropharyngeal swab
Gerardo Petruzzi, Armando De Virgilio, Barbara Pichi, Francesco Mazzola, Jacopo Zocchi, Giuseppe Mercante, Giuseppe Spriano, Raul Pellini, Gerardo Petruzzi, Armando De Virgilio, Barbara Pichi, Francesco Mazzola, Jacopo Zocchi, Giuseppe Mercante, Giuseppe Spriano, Raul Pellini
Abstract
Performing a proper nasal and oropharyngeal swab procedure is essential in the screening of COVID-19 infection. The video illustration of nasal and oropharyngeal swab is presented (Video S1). To correctly perform the nasopharyngeal swab, the patient must be seated comfortably with the back of their head against the headrest. The swab is inserted in the nose horizontally, along an imaginary line between the nostril and the ear. Oropharyngeal sampling is easier to perform. The swab is directed toward the rear wall of the oropharynx and it is rotated a few times before removal. After taking the sample, it is necessary to insert both swabs in the same tube, breaking the rod with one swift and controlled movement. Finally, carefully reset the cap. It appears to be extremely important to properly collect nasopharyngeal and oropharyngeal swabs in order to minimize the false negative rate among COVID-19 positive patients.
Keywords: COVID-19; coronavirus; nasopharynx; oropharynx; swab.
Conflict of interest statement
The authors declare no potential conflict of interests.
© 2020 Wiley Periodicals, Inc.
References
- Loeffelholz MJ, Tang YW. Laboratory diagnosis of emerging human coronavirus infections—the state of the art. Emerg Microbes Infect. 2020;9:747‐756. 10.1080/22221751.2020.1745095.
- Chen Y, Chen L, Deng Q, et al. The presence of SARS‐CoV‐2 RNA in feces of COVID‐19 patients. J Med Virol. 2020. 10.1002/jmv.25825.
- Gaunt ER, Hardie A, Claas EC, et al. Epidemiology and clinical presentations of the four human coronaviruses 229E, HKU1, NL63, and OC43 detected over 3 years using a novel multiplex real‐time PCR method. J Clin Microbiol. 2010;48(8):2940‐2947. 10.1128/JCM.00636–00610.
- Charlton CL, Babady E, Ginocchio CC, et al. Practical guidance for clinical microbiology laboratories: viruses causing acute respiratory tract infections. Clin Microbiol Rev. 2019;32(1):e00042‐18. 10.1128/CMR.00042-18.
Source: PubMed