Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling
Khem Bahadur Adhikari, Morten Rohde, Sten Velschow, Ulla Feldt-Rasmussen, Jesper Johannesen, Anders Holten Johnsen, Khem Bahadur Adhikari, Morten Rohde, Sten Velschow, Ulla Feldt-Rasmussen, Jesper Johannesen, Anders Holten Johnsen
Abstract
Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.
Keywords: LC–MS/MS; cortisol; dried blood spot; fluispotter; hematocrit; microsampling.
Source: PubMed