Effect of bio-oss collagen and collagen matrix on bone formation

R W K Wong, A B M Rabie, R W K Wong, A B M Rabie

Abstract

Objective: to compare the amount of new bone produced by Bio-Oss((R)) Collagen to that produced by collagen matrix in vivo.

Method: eighteen bone defects, 5mm by 10mm were created in the parietal bone of 9 New Zealand White rabbits. 6 defects were grafted with Bio-Oss((R)) Collagen. 6 defects were grafted with collagen matrix alone (positive control) and 6 were left empty (negative control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation was made on 100 sections (50 sections for each group) using image analysis.

Results: A total of 339% more new bone was present in defects grafted with Bio-Oss((R)) Collagen than those grafted with collagen matrix (positive control). No bone was formed in the negative control group.

Conclusion: Bio-Oss((R)) Collagen has the effect of stimulating new bone formation locally compared with collagen matrix in vivo. Bio-Oss((R) )Collagen may be utilized as a bone graft material.

Keywords: Bio-Oss® Collagen.; Bone graft; Collagen.

Figures

Fig. (1)
Fig. (1)
diagram of the dorsal view of the skull of a rabbit, showing the sites of two surgically-created bone defects.
Fig. (2)
Fig. (2)
photomicrograph showing healing of a bony defect grafted with Bio-Oss® Collagen on day 14. New bone (N) can be seen near the margins of the defect. H = host bone. Some Bio-Oss® granules (B) remained at the centre of the bony defect (periodic acid-Schiff stain, original magnification ×40). Image inside box was enlarged in Fig. (3).
Fig. (3)
Fig. (3)
high power photomicrograph showing the formation of new bone in a bony defect grafted with Bio-Oss® Collagen (box in Fig. 2). New bone (N) with osteocytes (O) could be seen growing around the Bio-Oss® granules (B) from the margin of the host bone defect (H). Capillaries (cap) were present. No cartilage was found. (periodic acid-Schiff stain, original magnification ×200).
Fig. (4)
Fig. (4)
photomicrograph of defect grafted with collagen matrix in day 14. No bone could be seen across the defect except for a little new bone (N) near the ends of the host bone (H). Collagen matrix (C) remained across the bone defect (periodic acid-Schiff stain, original magnification ×40).

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