Plasma anandamide and other N-acylethanolamines are correlated with their corresponding free fatty acid levels under both fasting and non-fasting conditions in women

Michel M Joosten, Michiel Gj Balvers, Kitty Cm Verhoeckx, Henk Fj Hendriks, Renger F Witkamp, Michel M Joosten, Michiel Gj Balvers, Kitty Cm Verhoeckx, Henk Fj Hendriks, Renger F Witkamp

Abstract

N-acylethanolamines (NAEs), such as anandamide (AEA), are a group of endogenous lipids derived from a fatty acid linked to ethanolamine and have a wide range of biological activities, including regulation of metabolism and food intake. We hypothesized that i) NAE plasma levels are associated with levels of total free fatty acids (FFAs) and their precursor fatty acid in fasting and non-fasting conditions and ii) moderate alcohol consumption alters non-fasting NAE levels. In a fasting and non-fasting study we sampled blood for measurements of specific NAEs and FFAs. In the fasting study blood was drawn after an overnight fast in 22 postmenopausal women. In the non-fasting study blood was sampled before and frequently after a standardized lunch with beer or alcohol-free beer in 19 premenopausal women. Fasting AEA levels correlated with total FFAs (r = 0.84; p < 0.001) and arachidonic acid levels (r = 0.42; p < 0.05). Similar results were observed for other NAEs with both total FFAs and their corresponding fatty acid precursors. In addition, AEA (r = 0.66; p < 0.01) and OEA levels (r = 0.49; p <0.02) positively related with BMI. Changes over time in non-fasting AEA levels were correlated with changes in total FFA levels, both after a lunch with beer (r = 0.80; 95% confidence interval: 0.54-0.92) and alcohol-free beer (r = 0.73; 0.41-0.89). Comparable correlations were found for other NAEs, without differences in correlations of each NAE between beer and alcohol free beer with lunch. In conclusion, i) in fasting and non-fasting states circulating anandamide and other N-acylethanolamines were associated with free fatty acid levels and ii) moderate alcohol consumption does not affect non-fasting NAE levels. This suggests that similar physiological stimuli cause the release of plasma N-acylethanolamines and free fatty acids in blood. The trials are registered at ClinicalTrials.gov numbers: NCT00524550 and NCT00652405.

Figures

Figure 1
Figure 1
Structures of the N-acylethanolamines: anandamide (AEA), palmitoylethanolamide (PEA), oleoylethanolamide (OEA) and stearoylethanolamide (SEA).
Figure 2
Figure 2
Pearson correlations between plasma levels of several N-acylethanolamines and their corresponding serum levels of fatty acid in twenty-two postmenopausal women after an overnight fast.
Figure 3
Figure 3
Means (±SEM) of serum free fatty acid (FFA) concentrations and plasma concentrations of anandamide (AEA), palmitoylethanolamide (PEA) and oleoylethanolamide (OEA) before and during three hours after a lunch with beer (black circles) or alcohol-free beer consumption (open circles) in 19 normal-weight premenopausal women.

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