Identification of Haplotype Tag Single-Nucleotide Polymorphisms within the PPAR Family Genes and Their Clinical Relevance in Patients with Major Trauma

Jun-Wei Gao, Ling Zeng, An-Qiang Zhang, Xiao Wang, Wei Pan, Ding-Yuan Du, Lian-Yang Zhang, Wei Gu, Jian-Xin Jiang, Jun-Wei Gao, Ling Zeng, An-Qiang Zhang, Xiao Wang, Wei Pan, Ding-Yuan Du, Lian-Yang Zhang, Wei Gu, Jian-Xin Jiang

Abstract

Background: Peroxisome proliferator-activated receptors (PPARs) play important roles in the development of inflammatory diseases and sepsis. Recently, genetic variants of PPARs genes have been widely studied in some inflammatory diseases. However, the association between PPAR family of genes polymorphisms and sepsis risk in trauma patients was little known.

Methods: SNPs were selected from the PPARs genes through constructing haplotype blocks and genotyped by the improved multiplex ligation detection reaction (iMLDR) method. The association between the selected SNPs and the risk of sepsis and multiple organ dysfunction (MOD) scores was evaluated in 734 trauma patients. In addition, tumor necrosis factor α (TNFα) production of peripheral blood leukocytes was also analyzed after lipopolysaccharide (LPS) stimulation.

Results: Our results revealed that there were significant associations between the rs10865710 polymorphism and the risk of sepsis and MOD scores in Chinese Han trauma patients. Further, we found that the level of TNFα production was higher in patients with the rs10865710 G allele compared to those with the variant C allele.

Conclusions: The rs10865710 polymorphism in the PPARγ gene might be used to assess the risk of sepsis and multiple organ dysfunction syndrome (MODS) in trauma patients.

Keywords: MODS; genetic polymorphism; peroxisome proliferator-activated receptor; sepsis; trauma.

Figures

Figure 1
Figure 1
Effect of the PPARγ rs10865710 polymorphism on LPS-induced TNFα production (p = 0.041 for the dominant model; p = 0.027 for the recessive model).

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