Lack of pathogenic germline DICER1 variants in males with testicular germ-cell tumors
Lauren M Vasta, Mary L McMaster, Laura A Harney, Alexander Ling, Jung Kim, Anne K Harris, Ann G Carr, Scott M Damrauer, Daniel J Rader, Rachel L Kember, Peter A Kanetsky, Katherine L Nathanson, Louise C Pyle, Mark H Greene, Kris Ann Schultz, Douglas R Stewart, Regeneron Genetics Center (RGC) Research Team, Lauren M Vasta, Mary L McMaster, Laura A Harney, Alexander Ling, Jung Kim, Anne K Harris, Ann G Carr, Scott M Damrauer, Daniel J Rader, Rachel L Kember, Peter A Kanetsky, Katherine L Nathanson, Louise C Pyle, Mark H Greene, Kris Ann Schultz, Douglas R Stewart, Regeneron Genetics Center (RGC) Research Team
Abstract
Background: Several studies have reported conflicting evidence on the inclusion of testicular germ cell tumors (TGCT) in the DICER1 tumor-predisposition phenotype. We evaluated the relationship between DICER1 and TGCT by reviewing scrotal ultrasounds of males with pathogenic germline variants in DICER1 and queried exome data from TGCT-affected men for DICER1 variants.
Methodology: Fifty-four male DICER1-carriers and family controls (n=41) enrolled in the National Cancer Institute (NCI) DICER1 Natural History Study were offered scrotal ultrasounds. These studies were examined by a single radiologist for abnormalities. In parallel, DICER1 variants from two large exome-sequenced TGCT cohorts were extracted. We used previously published AMG-AMP criteria to characterize rare DICER1 variants.
Results: There was no observed difference in frequency of testicular cystic structures in DICER1-carriers versus controls. DICER1 variation was not associated with TGCT in the NCI DICER1-carriers. In 1,264 exome-sequenced men with TGCT, none harbored ClinVar- or InterVar-determined pathogenic or likely pathogenic variants in DICER1. Three DICER1 variants of uncertain significance (one case and two controls) were predicted "damaging" based on a priori criteria.
Conclusion: Using two complementary approaches, we found no evidence of an association between pathogenic DICER1 variants and TGCT.
Keywords: DICER1; Exome sequencing; Scrotal ultrasound; Testicular cancer; miRNA.
Conflict of interest statement
Declaration of Competing Interest DRS provides contract clinical tele-genetics services to Genome Medical Inc. in accordance with relevant NCI ethics policies. SMD receives research support to his institution from RenalytixAI and personal consulting fees from Calico Labs; all outside the current work.
Copyright © 2020. Published by Elsevier Inc.
Source: PubMed