Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome
28. august 2021 opdateret af: Jessica Ellis, East Carolina University
Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome in Lean and Overweight or Obese Subjects
The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.
Studieoversigt
Status
Afsluttet
Betingelser
Intervention / Behandling
Detaljeret beskrivelse
After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study.
All participants then gave a muscle sample and ate a calorically controlled breakfast.
Participants completed ~1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.
Undersøgelsestype
Interventionel
Tilmelding (Faktiske)
30
Fase
- Ikke anvendelig
Kontakter og lokationer
Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.
Studiesteder
-
-
Indiana
-
West Lafayette, Indiana, Forenede Stater, 47907
- Purdue University
-
-
Deltagelseskriterier
Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.
Berettigelseskriterier
Aldre berettiget til at studere
18 år til 30 år (Voksen)
Tager imod sunde frivillige
Ja
Køn, der er berettiget til at studere
Han
Beskrivelse
Inclusion Criteria:
- healthy males that completed a physical examination with a State Licensed M.D. or D.O. within the past 2 years and completed a physical activity readiness questionnaire (PARQ), the International Physical Activity Questionnaires (IPAQ) and and a medical history questionnaire prior to the study
Exclusion Criteria:
- any participants that had not had a physical examination by a State Licensed M.D. or D.O. within the past 2 years
- persons not considered "healthy" (with any previous medical diagnoses) or not medically cleared by a M.D. or D.O. to perform physical activity/exercise
- persons who are regularly physically active
Studieplan
Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Grundvidenskab
- Tildeling: Ikke-randomiseret
- Interventionel model: Parallel tildeling
- Maskning: Enkelt
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
|---|---|
|
Aktiv komparator: Lean
males aged 18-30 years with BMI < 25
|
All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
|
|
Eksperimentel: Overweight/Obese
males aged 18-30 years with BMI >= 25
|
All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
|
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
|
Change from baseline in skeletal muscle acylcarnitines
Tidsramme: Baseline and immediately after acute cycling exercise
|
Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels.
|
Baseline and immediately after acute cycling exercise
|
|
Change from baseline in skeletal transcriptome
Tidsramme: Baseline and immediately after acute cycling exercise
|
RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes.
|
Baseline and immediately after acute cycling exercise
|
|
Change from baseline in skeletal muscle whole genome nucleosome maps
Tidsramme: Baseline and immediately after acute cycling exercise
|
MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing.
|
Baseline and immediately after acute cycling exercise
|
Sekundære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
|
Glucose
Tidsramme: Baseline and immediately after acute cycling exercise
|
Commercially validated and available colorimetric used to determine plasma glucose levels.
|
Baseline and immediately after acute cycling exercise
|
|
Insulin
Tidsramme: Baseline and immediately after acute cycling exercise
|
Commercially validated and available ELISAs used to determine plasma insulin levels.
|
Baseline and immediately after acute cycling exercise
|
|
Triglycerides
Tidsramme: Baseline and immediately after acute cycling exercise
|
Commercially validated and available colorimetric kits used to determine plasma triglyceride levels.
|
Baseline and immediately after acute cycling exercise
|
|
Free fatty acids
Tidsramme: Baseline and immediately after acute cycling exercise
|
Commercially validated and available colorimetric kits used to determine plasma free fatty acid levels.
|
Baseline and immediately after acute cycling exercise
|
|
Cholesterol
Tidsramme: Baseline and immediately after acute cycling exercise
|
Commercially validated and available colorimetric kits used to determine total cholesterol, HDL, LDL and VLDL levels in plasma.
|
Baseline and immediately after acute cycling exercise
|
Samarbejdspartnere og efterforskere
Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.
Sponsor
Efterforskere
- Ledende efterforsker: Tara Henagan, PhD, Purdue University, Louisiana State University Medical School at Shreveport
Datoer for undersøgelser
Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.
Studer store datoer
Studiestart (Faktiske)
14. september 2014
Primær færdiggørelse (Faktiske)
6. oktober 2015
Studieafslutning (Faktiske)
14. marts 2016
Datoer for studieregistrering
Først indsendt
16. september 2020
Først indsendt, der opfyldte QC-kriterier
28. august 2021
Først opslået (Faktiske)
5. september 2021
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
5. september 2021
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
28. august 2021
Sidst verificeret
1. august 2021
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- AcuteExerciseNucleo
Lægemiddel- og udstyrsoplysninger, undersøgelsesdokumenter
Studerer et amerikansk FDA-reguleret lægemiddelprodukt
Ingen
Studerer et amerikansk FDA-reguleret enhedsprodukt
Ingen
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