Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome

Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome in Lean and Overweight or Obese Subjects

The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.

Study Overview

• Status: Completed
• Conditions: Cardiovascular Diseases; Obesity; Type 2 Diabetes; Acute Exercise
• Intervention / Treatment: Behavioral: Acute exercise

Detailed Description

After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study. All participants then gave a muscle sample and ate a calorically controlled breakfast. Participants completed ~1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.

• Study Type: Interventional
• Enrollment (Actual): 30
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Indiana
    • West Lafayette, Indiana, United States, 47907
      • Purdue University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• healthy males that completed a physical examination with a State Licensed M.D. or D.O. within the past 2 years and completed a physical activity readiness questionnaire (PARQ), the International Physical Activity Questionnaires (IPAQ) and and a medical history questionnaire prior to the study

Exclusion Criteria:

• any participants that had not had a physical examination by a State Licensed M.D. or D.O. within the past 2 years
• persons not considered "healthy" (with any previous medical diagnoses) or not medically cleared by a M.D. or D.O. to perform physical activity/exercise
• persons who are regularly physically active

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Lean; males aged 18-30 years with BMI < 25	Behavioral: Acute exercise; All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
Experimental: Overweight/Obese; males aged 18-30 years with BMI >= 25	Behavioral: Acute exercise; All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change from baseline in skeletal muscle acylcarnitines	Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels.	Baseline and immediately after acute cycling exercise
Change from baseline in skeletal transcriptome	RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes.	Baseline and immediately after acute cycling exercise
Change from baseline in skeletal muscle whole genome nucleosome maps	MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing.	Baseline and immediately after acute cycling exercise

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Glucose	Commercially validated and available colorimetric used to determine plasma glucose levels.	Baseline and immediately after acute cycling exercise
Insulin	Commercially validated and available ELISAs used to determine plasma insulin levels.	Baseline and immediately after acute cycling exercise
Triglycerides	Commercially validated and available colorimetric kits used to determine plasma triglyceride levels.	Baseline and immediately after acute cycling exercise
Free fatty acids	Commercially validated and available colorimetric kits used to determine plasma free fatty acid levels.	Baseline and immediately after acute cycling exercise
Cholesterol	Commercially validated and available colorimetric kits used to determine total cholesterol, HDL, LDL and VLDL levels in plasma.	Baseline and immediately after acute cycling exercise

Collaborators and Investigators

• Sponsor: East Carolina University
• Collaborators: Purdue University; Louisiana State University Health Sciences Center Shreveport
• Investigators: Principal Investigator: Tara Henagan, PhD, Purdue University, Louisiana State University Medical School at Shreveport

Study record dates

Study Major Dates

• Study Start (Actual): September 14, 2014
• Primary Completion (Actual): October 6, 2015
• Study Completion (Actual): March 14, 2016

Study Registration Dates

• First Submitted: September 16, 2020
• First Submitted That Met QC Criteria: August 28, 2021
• First Posted (Actual): September 5, 2021

Study Record Updates

• Last Update Posted (Actual): September 5, 2021
• Last Update Submitted That Met QC Criteria: August 28, 2021
• Last Verified: August 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Cardiovascular Diseases
• Other Study ID Numbers: AcuteExerciseNucleo

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No