Wolbachia Reduces the Transmission Potential of Dengue-Infected Aedes aegypti

Yixin H Ye, Alison M Carrasco, Francesca D Frentiu, Stephen F Chenoweth, Nigel W Beebe, Andrew F van den Hurk, Cameron P Simmons, Scott L O'Neill, Elizabeth A McGraw, Yixin H Ye, Alison M Carrasco, Francesca D Frentiu, Stephen F Chenoweth, Nigel W Beebe, Andrew F van den Hurk, Cameron P Simmons, Scott L O'Neill, Elizabeth A McGraw

Abstract

Background: Dengue viruses (DENV) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. Wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. The Wolbachia strain wMel, which has been introduced into the mosquito vector, Aedes aegypti, has been shown to invade and spread to near fixation in field releases. Standard measures of Wolbachia's efficacy for blocking virus replication focus on the detection and quantification of virus in mosquito tissues. Examining the saliva provides a more accurate measure of transmission potential and can reveal the extrinsic incubation period (EIP), that is, the time it takes virus to arrive in the saliva following the consumption of DENV viremic blood. EIP is a key determinant of a mosquito's ability to transmit DENVs, as the earlier the virus appears in the saliva the more opportunities the mosquito will have to infect humans on subsequent bites.

Methodology/principal findings: We used a non-destructive assay to repeatedly quantify DENV in saliva from wMel-infected and Wolbachia-free wild-type control mosquitoes following the consumption of a DENV-infected blood meal. We show that wMel lengthens the EIP, reduces the frequency at which the virus is expectorated and decreases the dengue copy number in mosquito saliva as compared to wild-type mosquitoes. These observations can at least be partially explained by an overall reduction in saliva produced by wMel mosquitoes. More generally, we found that the concentration of DENV in a blood meal is a determinant of the length of EIP, saliva virus titer and mosquito survival.

Conclusions/significance: The saliva-based traits reported here offer more disease-relevant measures of Wolbachia's effects on the vector and the virus. The lengthening of EIP highlights another means, in addition to the reduction of infection frequencies and DENV titers in mosquitoes, by which Wolbachia should operate to reduce DENV transmission in the field.

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1. Extrinsic incubation period (DPI) of…
Fig 1. Extrinsic incubation period (DPI) of WT (white) and Wolbachia infected (wMel.F) mosquitoes (grey).
Bars depict means ± S.E.M. Mosquitoes were orally infected with either 107 or 106 PFU/ml of DENV. Each data point represents a pool of 10 mosquitoes.
Fig 2. Number of days infective saliva…
Fig 2. Number of days infective saliva was detected for WT (white) and wMel.F mosquitoes (grey).
Bars depict medians ± interquartile range. Mosquitoes were orally infected with either 107 or 106 PFU/ml of DENV. Each data point represents a pool of 10 mosquitoes.
Fig 3. The DENV titer in the…
Fig 3. The DENV titer in the sucrose solution of WT (white bar) and wMel.F mosquitoes (grey) fed on 107 (A) or 106 (B) PFU/ml of DENV.
Bars depict medians ± interquartile range. DENV titer is expressed as copies per live individual to correct for death.
Fig 4. Survival curves of WT (grey…
Fig 4. Survival curves of WT (grey line) and wMel.F mosquitoes (black line) orally infected with either 107 (A) or 106 (B) PFU/ml of DENV.
Bars depict means ± S.E.M.
Fig 5. Percentage of replicates infective through…
Fig 5. Percentage of replicates infective through time ± confidence intervals for WT (grey line) and wMel.F mosquitoes (black line) orally infected with either 107 (A) or 106 (B) PFU/ml of DENV.
Fig 6. Comparison of saliva volume of…
Fig 6. Comparison of saliva volume of WT (white) and wMel.F mosquitoes (grey) of different ages.
Saliva volume is measure through the sphere volume of saliva droplets using mineral oil. Bars depict means ±S.E.M. Number of replicates range from 33 to 80 with a mean of 62 individuals for WT mosquitoes and 34 to 85 with a mean of 64 for wMel.F mosquitoes. 86.8% of all the mosquitoes produced saliva during the assay.
Fig 7. Comparison of sucrose feeding frequency…
Fig 7. Comparison of sucrose feeding frequency of WT (white) and wMel.F mosquitoes (grey) at four different ages.
Bars depict means ±S.E.M. Number of replicates range from 26 to 40 with a mean of 37 individuals for WT mosquitoes and 20 to 40 with a mean of 35 for wMel.F mosquitoes across all time points. 80.1% of all the mosquitoes produced feeding frequency data.

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