Formulation and in vivo evaluation for anti-aging effects of an emulsion containing basil extract using non- invasive biophysical techniques

A Rasul, N Akhtar, A Rasul, N Akhtar

Abstract

BACKGROUND AND THE PURPOSE OF STUDY: Skin aging is a complex process induced by constant exposure to ultraviolet (UV) irradiation and damages human skin. UV generates reactive oxygen species leading to collagen deficiency and eventually skin wrinkling. Basil contains a number of phenolics and favonoids which possess antioxidant properties. The aim of this study was to formulate and investigate the antiaging potential of a cream containing Basil extract.

Methods: A single blinded study was conducted using non-invasive methods. Formulation containing 3% of the concentrated extract of Basil was developed by entrapping in the inner aqueous phase of w/o emulsion and base contained no extract. Both creams were stored at different storage conditions of 8°C, 25°C, 40°C and 40°C+ 75% relative humidity to predict their stabilities. The formulation and base were evaluated for their effects on various skin parameters i.e., moisture and trans epidermal water loss (TEWL), volume, energy and surface evaluation of the living skin (SELS).

Results: Significant effects (p≤0.05) were observed for both creams in the case of TEWL. The base showed insignificant (p≤0.05) while formulation showed significant effects on skin moisture. Volume, SELS SEr (skin roughness), SEsc (skin scaliness), SEsm (skin smoothness), SEw (skin wrinkles) parameter showed significant decline while texture parameter of 'Energy' showed significant increase.

Conclusion: The results statistically indicated that the active formulation containg extract of Basil exert antiaging effects when applied topically.

Keywords: Ocimum basilicum; Surface evaluation of living skin (SELS); Transepidermal water loss (TEWL); w/o emulsion.

Figures

Figure 1
Figure 1
Percentage of change in skin moisture content after application of base and formulation
Figure 2
Figure 2
Percentage of change in TEWL after application of base and formulation.
Figure 3
Figure 3
Percentage of change in volume after application of base and formulation.
Figure 4
Figure 4
Percentage of change in energy after application of base and formulation.
Figure 5
Figure 5
Percentage of change in mean VC 98 units of SELS parameters after application of base and formulation. 1B; Base values after one month, 1F; Formulation values after one month, 2B; Base values after two months, 2F; Formulation values after two months, 3B; Base values after three months, 3F; Formulation values after three months

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