The Human Microbiome Project: a community resource for the healthy human microbiome

Dirk Gevers, Rob Knight, Joseph F Petrosino, Katherine Huang, Amy L McGuire, Bruce W Birren, Karen E Nelson, Owen White, Barbara A Methé, Curtis Huttenhower, Dirk Gevers, Rob Knight, Joseph F Petrosino, Katherine Huang, Amy L McGuire, Bruce W Birren, Karen E Nelson, Owen White, Barbara A Methé, Curtis Huttenhower

Abstract

This manuscript describes the NIH Human Microbiome Project, including a brief review of human microbiome research, a history of the project, and a comprehensive overview of the consortium's recent collection of publications analyzing the human microbiome.

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1. Timeline of microbial community studies…
Figure 1. Timeline of microbial community studies using high-throughput sequencing.
Each circle represents a high-throughput sequence-based 16S or shotgun metagenomic bioproject in NCBI (May 2012), indicating the amount of sequence data produced for each project (circle area and y-coordinate) at the time of publication/registration (x-coordinate). Projects are grouped by human-associated (red), other animal (black), or environmental (green) communities, and shotgun metagenomic projects are marked with a grey band. Selected representative projects are labeled: open ocean , deep sea , lean mouse , diarrheal illness , costal ocean , lean/obese gut , human microbiome , MetaHIT (gut) , cow rumen , soil (NCBI BioProject PRJNA50473), and human gut . Note that HMP has deposited a total of 7.44 terabases of shotgun data in SRA, of which 49% is host DNA derived data that was filtered and only available through protected access in dbGaP project phs000228.
Figure 2. HMP consortium activities as a…
Figure 2. HMP consortium activities as a model for microbiome data generation and analyses.
(A) Initiatives within the HMP coordinated to isolate samples, generate data, perform analysis, and publish results. Technology development was employed to develop novel bacterial culture and DNA isolation techniques. Ethical Legal and Social Implications (ELSI) work anticipated societal implications and guided policies associated with human subject microbiomes. Clinical sites were collected samples from large cohorts of healthy individuals, with nucleotide sequence information derived at four sequencing centers at the Baylor College of Medicine (BCM), the Broad Institute, the J. Craig Venter Institute (JCVI), and the Washington University Genome Institute (WUGI). Additional demonstration projects assessed primarily microbiome alterations related to disease. In addition to analysis throughout the HMP consortium, computational tools were funded to address, for example, genome assembly, microbial ecology, and statistical modeling. A data analysis and coordination center provided a portal to all data generated. (B) Overview of the analysis approaches that were the ultimate product of the HMP consortium, corresponding to data products and protocols available at http://hmpdacc.org.

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