Variability of anti-staphylococcal antibodies in healthy volunteers and pre-cardiac surgery patients

Sarka Moravcova, Bonnie Kyle, Hilary Shanahan, Savvas Giannaris, Andrew Smith, Colin Hamilton-Davies, Sarka Moravcova, Bonnie Kyle, Hilary Shanahan, Savvas Giannaris, Andrew Smith, Colin Hamilton-Davies

Abstract

Background: Pre-operative antibody levels have been shown to be inversely related to development of post-operative complications. Staphylococcal infection is a major source of morbidity following surgery.

Methods: We examined the variability of anti-staphylococcal antibody levels across a group of healthy volunteers and compared this with patients scheduled to undergo cardiac surgery.

Results: Pre-operative cardiac surgical patients exhibited significantly higher levels of staphylococcal antibodies compared with healthy volunteers.

Conclusions: The relationship between pre-surgery staphylococcal antibody levels and outcome warrants further investigation.

Keywords: Antibody; Complication; EndoCAb; Infection; Pre-operative; Staphylococcus.

Figures

Fig. 1
Fig. 1
Categorised antibody response (positive, weakly positive, negative) to alpha-toxin and teichoic acid domains in healthy volunteers (HV), n = 25, and in pre-operative cardiac surgical patients (PS), n = 25. Fisher’s exact test (Freeman-Halton extension) used to test variability of response between groups

References

    1. Allen KB, Fowler VG, Jr, Gammie JS, et al. Staphylococcus aureus infections after elective cardiothoracic surgery: observations from an international randomized placebo-controlled trial of an investigational S. aureus vaccine. Open Forum Infect Dis. 2014;1(2):1–7. doi: 10.1093/ofid/ofu071.
    1. Barclay GR. Bacterial endotoxins: lipopolysaccharides from genes to therapy. In: Levin J, Alving CR, Munford RS, Redl H, editors. Progress in clinical and biological research. New York: Wiley; 1995.
    1. Bennett-Guerrero E, Ayuso L, Hamilton-Davies C, et al. Relationship of preoperative antiendotoxin core antibodies and adverse outcomes following cardiac surgery. JAMA. 1997;277(8):646–50. doi: 10.1001/jama.1997.03540320048034.
    1. Colque-Navarro P, Soderquist B, Holmberg H, Blomqvist L, Olcen P, Mollby R. Antibody response in Staphylococcus aureus septicaemia—a prospective study. J Med Microbiol. 1998;47(3):217–25. doi: 10.1099/00222615-47-3-217.
    1. Coskun D, Aytac J, Aydinli A, Bayer A. Mortality rate, length of stay and extra cost of sternal surgical site infections following coronary artery bypass grafting in a private medical centre in Turkey. J Hosp Infect. 2005;60(2):176–9. doi: 10.1016/j.jhin.2004.10.017.
    1. Down JF, Barclay GR, Bennett-Guerrero E, et al. A descriptive study of the variation in baseline levels of antiendotoxin core antibodies between US and UK populations. J Endotoxin Res. 2004;10(3):195–6.
    1. Dryla A, Prustomersky S, Gelbmann D, et al. Comparison of antibody repertoires against Staphylococcus aureus in healthy individuals and in acutely infected patients. Clin Diagn Lab Immunol. 2005;12(3):387–98.
    1. Fowler VG, Jr, O’Brien SM, Muhlbaier LH, Corey GR, Ferguson TB, Peterson ED. Clinical predictors of major infections after cardiac surgery. Circulation. 2005;112(9 Suppl):I358–65.
    1. Hamilton-Davies C, Barclay GR, Cardigan RA, et al. Relationship between preoperative endotoxin immune status, gut perfusion, and outcome from cardiac valve replacement surgery. Chest. 1997;112(5):1189–96. doi: 10.1378/chest.112.5.1189.
    1. Haq K, McElhaney JE. Immunosenescence: influenza vaccination and the elderly. Curr Opin Immunol. 2014;29:38–42. doi: 10.1016/j.coi.2014.03.008.
    1. Kanafani ZA, Arduino JM, Muhlbaier LH, et al. Incidence of and preoperative risk factors for Staphylococcus aureus bacteremia and chest wound infection after cardiac surgery. Infect Control Hosp Epidemiol. 2009;30(3):242–8. doi: 10.1086/596015.
    1. Kwak YK, Vikstrom E, Magnusson KE, Vecsey-Semjen B, Colque-Navarro P, Mollby R. The Staphylococcus aureus alpha-toxin perturbs the barrier function in Caco-2 epithelial cell monolayers by altering junctional integrity. Infect Immun. 2012;80(5):1670–80. doi: 10.1128/IAI.00001-12.
    1. Mollby R. Isolation and properties of membrane damaging toxins. In: Easmon CSF, Adlam C, editors. Staphylococci and staphylococcal infections. 2. London: Academic; 1983. pp. 628–30.
    1. Ridderstolpe L, Gill H, Granfeldt H, Ahlfeldt H, Rutberg H. Superficial and deep sternal wound complications: incidence, risk factors and mortality. Eur J Cardiothorac Surg. 2001;20(6):1168–75. doi: 10.1016/S1010-7940(01)00991-5.
    1. Soderquist BC-N P, Blomqvist L, Olcen P, Holmberg H, Mollby R. Enzyme immunoassay for detection of ∝-toxin from staphylococcus aureus. Serodiagn Immunother Infect Dis. 1993;1:23–6. doi: 10.1016/0888-0786(93)90020-Z.

Source: PubMed

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