Comparison of vascular parameters between normal cynomolgus macaques and healthy humans by optical coherence tomography angiography

Jingyi Peng, Liuxueying Zhong, Li Ma, Jiayi Jin, Yongxin Zheng, Chenjin Jin, Jingyi Peng, Liuxueying Zhong, Li Ma, Jiayi Jin, Yongxin Zheng, Chenjin Jin

Abstract

Background: The metabolic activity of retina is higher than other human tissues and is crucial to the vision. Cynomolgus macaques is widely used in ophthalmic disease research. The evaluation and comparison of macular and optic disc vascular circulation parameters between normal adult cynomolgus macaques and healthy adult humans using OCT-A can promote better use of nonhuman primate models in studies of ophthalmic vascular disease.

Methods: Twelve normal adult cynomolgus macaques with a mean age of 4.91 ± 0.43 years were studied for data collection. The macula of 28 adult healthy humans (14 males and 14 females), with a mean age of 25.11 ± 6.21 years and the optic discs of 9 adult healthy humans (4 males and 5 females) with a mean age of 28.56 ± 6.78 years were measured. The vessel density (VD) was measured using an RTVue XR with AngioVue. The scan sizes of the macular and optic discs were 3 × 3 mm and 4.5 × 4.5 mm, respectively.

Results: OCT-A can image the superficial and deep capillary plexuses and radial peripapillary capillary network. In RPC layer of the optic disc, the VD in the nasal quadrant was lower than the VD in the inferior temporal quadrant. Similarities and significant differences in VD between healthy humans and cynomolgus macaques were obtained using OCT-A.

Conclusions: This study provides normal vascular parameters for adult cynomolgus macaques using OCT-A to help establish an optical parameter database for cynomolgus macaques and compare VD between healthy humans and cynomolgus macaques to promote choroid-retinopathy research.

Trial registration: Current Controlled Trials NCT03692169 , retrospectively registered on 26 sept 2018.

Keywords: Cynomolgus macaques; Healthy humans; Optical coherence tomography angiography (OCT-A); Vascular; Vessel density (VD).

Conflict of interest statement

All authors are affiliated with the State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, Guangdong 510060, China. The author(s) have no potential competing interests to declare.

Figures

Fig. 1
Fig. 1
The OCT-A images of cynomolgus macaques and healthy humans in macular. a Retinal layer (b) Ring centering on macula with foveal ring (1 mm), four quadrants (superior, inferior, temporal, and nasal, 3 mm). (c) The En-face (d) Normal virtual colored macular vascular density
Fig. 2
Fig. 2
Histogram of vessel density (VD) in macula and optic disc of cynomolgus macaques and healthy humans
Fig. 3
Fig. 3
The OCT-A images of cynomolgus macaques and healthy humans in optic disc. Description: (a) Retinal layer (b) A ring with width of 0.75 mm, centering on the disc, automatically divided the scanned area into seven sections. c The En-face (d) Normal virtual colored vascular density

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