The CoLaus study: a population-based study to investigate the epidemiology and genetic determinants of cardiovascular risk factors and metabolic syndrome

Mathieu Firmann, Vladimir Mayor, Pedro Marques Vidal, Murielle Bochud, Alain Pécoud, Daniel Hayoz, Fred Paccaud, Martin Preisig, Kijoung S Song, Xin Yuan, Theodore M Danoff, Heide A Stirnadel, Dawn Waterworth, Vincent Mooser, Gérard Waeber, Peter Vollenweider, Mathieu Firmann, Vladimir Mayor, Pedro Marques Vidal, Murielle Bochud, Alain Pécoud, Daniel Hayoz, Fred Paccaud, Martin Preisig, Kijoung S Song, Xin Yuan, Theodore M Danoff, Heide A Stirnadel, Dawn Waterworth, Vincent Mooser, Gérard Waeber, Peter Vollenweider

Abstract

Background: Cardiovascular diseases and their associated risk factors remain the main cause of mortality in western societies. In order to assess the prevalence of cardiovascular risk factors (CVRFs) in the Caucasian population of Lausanne, Switzerland, we conducted a population-based study (Colaus Study). A secondary aim of the CoLaus study will be to determine new genetic determinants associated with CVRFs.

Methods: Single-center, cross-sectional study including a random sample of 6,188 extensively phenotyped Caucasian subjects (3,251 women and 2,937 men) aged 35 to 75 years living in Lausanne, and genotyped using the 500 K Affymetrix chip technology.

Results: Obesity (body mass index > or = 30 kg/m2), smoking, hypertension (blood pressure > or = 140/90 mmHg and/or treatment), dyslipidemia (high LDL-cholesterol and/or low HDL-cholesterol and/or high triglyceride levels) and diabetes (fasting plasma glucose > or = 7 mmol/l and/or treatment) were present in 947 (15.7%), 1673 (27.0%), 2268 (36.7%), 2113 (34.2%) and 407 (6.6%) of the participants, respectively, and the prevalence was higher in men than in women. In both genders, the prevalence of obesity, hypertension and diabetes increased with age.

Conclusion: The prevalence of major CVRFs is high in the Lausanne population in particular in men. We anticipate that given its size, the depth of the phenotypic analysis and the availability of dense genome-wide genetic data, the CoLaus Study will be a unique resource to investigate not only the epidemiology of isolated, or aggregated CVRFs like the metabolic syndrome, but can also serve as a discovery set, as well as replication set, to identify novel genes associated with these conditions.

Figures

Figure 1
Figure 1
Flow chart of the CoLaus Study.
Figure 2
Figure 2
Power estimates for the CoLaus study. Power for various sample sizes in unmatched case-control studies. Arbitrary allelic frequency of 0.3, a disease prevalence of 50% and a type 1 error rate of 10-7taking into account 500'000 genetics markers. Curves are for estimated genetic effects (odds ratios) of 1.2 to 1.8. The numbers of cases for main CVRFs in the CoLaus study are: Hypertension: 2268. Dyslipidemia: 2021. Obesity: 963. Smoking (> 25 cigarettes/day): 746. Type 2 diabetes: 398. Coronary heart disease: 262. Low HDL: 170.
Figure 3
Figure 3
Power estimates for the CoLaus study. Power for the analysis of systolic blood pressure as a continuous variable. Power calculations using a continuous outcome for independent subjects, with an additive mode of action for the allele and a type 1 error rate of 10-7. Calculations were done for various minor allele frequencies (0.1 to 0.5).

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