PiggyBac transposon vectors: the tools of the human gene encoding

Shuang Zhao, Enze Jiang, Shuangshuang Chen, Yuan Gu, Anna Junjie Shangguan, Tangfeng Lv, Liguo Luo, Zhenghong Yu, Shuang Zhao, Enze Jiang, Shuangshuang Chen, Yuan Gu, Anna Junjie Shangguan, Tangfeng Lv, Liguo Luo, Zhenghong Yu

Abstract

A transposon is a DNA segment, which is able to change its relative position within the entire genome of a cell. The piggyBac (PB) transposon is a movable genetic element that efficiently transposes between vectors and chromosomes through a "cut-and-paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeats (ITRs) sequences located on both ends of the transposon vector and eight efficiently moves the contents from its original positions and efficiently integrates them into TTAA chromosomal sites. PB has drawn much attention because of its transposition efficiency, safety and stability. Due to its priorities, PB can be used as a new genetic vehicle, a new tool for oncogene screening and a new method for gene therapy. PB has created a new outlook for human gene encoding.

Keywords: PiggyBac (PB); transgene; transposon.

Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Transposons are assigned to one of two classes according to their mechanism of transposition. Retrotransposons copy themselves first from DNA to RNA by transcription, then from RNA to DNA by reverse transcription, and the DNA copy is then inserted into the genome in a new position through a “copy-and-paste” mechanism, while DNA transposons through a “cut-and-paste” mechanism, without creating a second copy of the DNA during transposition.
Figure 2
Figure 2
The piggyBac (PB) elements can be cut down from the donor chromosome by the transposase, and the split donor DNA was then reconnected with DNA ligase. After that, the vector was inserted into the target insertion site (TTAA), complete the transposition.

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Source: PubMed

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