Resveratrol-procyanidin blend: nutraceutical and antiaging efficacy evaluated in a placebocontrolled, double-blind study

Daniela Buonocore, Angelo Lazzeretti, Pedro Tocabens, Vincenzo Nobile, Enza Cestone, Giada Santin, Maria G Bottone, Fulvio Marzatico, Daniela Buonocore, Angelo Lazzeretti, Pedro Tocabens, Vincenzo Nobile, Enza Cestone, Giada Santin, Maria G Bottone, Fulvio Marzatico

Abstract

Background: Skin is constantly exposed to pro-oxidant environmental stress from several sources, including air pollutants, ultraviolet solar light, and chemical oxidants. Reactive oxygen species have been implicated in age-related skin disorders. Dietary bioactive antioxidant compounds, such as polyphenols, have beneficial effects on skin health. The advantage of a nutritional administration route is that blood delivers nutraceutical bioactive compounds continuously to all skin compartments, ie, the epidermis, dermis, and subcutaneous fat. The purpose of this study was to evaluate the topical and systemic effects of a dietary supplement containing resveratrol and procyanidin on age-related alterations to the skin, the skin antioxidant pool, and systemic oxidative stress levels.

Methods: An instrumental study was performed in 50 subjects (25 treated with supplements and 25 with placebo) to identify clinical features induced by chronoaging or photoaging. Product efficacy was evaluated after 60 days of treatment in terms of in vivo and in situ skin hydration, elasticity, and skin roughness levels, systemic oxidative stress levels by plasmatic derivatives of reactive oxygen metabolites and oxyadsorbent tests, and extent of the skin antioxidant pool.

Results: After 60 days of treatment, values for systemic oxidative stress, plasmatic antioxidant capacity, and skin antioxidant power had increased significantly. Additionally, skin moisturization and elasticity had improved, while skin roughness and depth of wrinkles had diminished. Intensity of age spots had significantly decreased, as evidenced by improvement in the individual typological angle.

Conclusion: Nutraceutical and pharmacological intervention with a supplement characterized by a specific blend of resveratrol and procyanidin may be a promising strategy to support treatments for the reduction of skin wrinkling, as well as reducing systemic and skin oxidative stress.

Keywords: antiaging; nutraceuticals; procyanidin; resveratrol supplementation; skin.

Figures

Figure 1
Figure 1
Antioxidant evaluation. (A) Derivatives of reactive oxygen metabolites test (Carratelli units). (B) Oxyadsorbent test (mmol HClO/L) both as a function of time in days (dd). Notes: Data are reported as the mean ± standard error of the mean. Intergroup statistical analysis (active versus placebo) is reported inside the bars of the histogram. The lines report the intragroup statistical analysis (T60 vs T0). Statistical analysis is marked with (*) in increasing numbers depending on significance. Abbreviation: d-ROMs, derivatives of reactive oxygen metabolites test.
Figure 2
Figure 2
Skin Antioxidant capacity: FRAP test- ( μmol Fe2+/L) as a function of time in days (dd). Notes: Data are reported as mean ± SEM. Intergroup statistical analysis (active vs placebo) is reported inside the bars of the histogram. The lines report the intragroup statistical analysis (T60 vs T0). Statistical analysis is marked with (*) in increasing numbers depending on significance. Abbreviation: FRAP test, ferric-reducing ability of plasma test.
Figure 3
Figure 3
Skin antiaging effect. (A) Skin moisturization (corneometric units). (B) Skin elasticity (R2 parameter) – (Ua/Uf ratio) both as a function of time in days (dd). Notes: Data are reported as the mean ± standard error of the mean. Intergroup statistical analysis (active versus placebo) is reported inside the bars of the histogram. The lines report the intragroup statistical analysis (T60 vs T0). Statistical analysis is marked with (*) in increasing numbers depending on significance.
Figure 4
Figure 4
Skin surface (μm). (A) Skin roughness. (B) Wrinkle depth, both as a function of time in days (dd). Notes: Data are reported as the mean ± standard error of the mean. Intergroup statistical analysis (active versus placebo) is reported inside the bars of the histogram. The lines report the intragroup statistical analysis (T60 vs T0). Statistical analysis is marked with (*) in increasing numbers depending on significance.
Figure 5
Figure 5
Intensity of brown spot: ITA° - grades (°) as a function of time in days (dd). Notes: Data are reported as the mean ± standard error of the mean. Intergroup statistical analysis (active versus placebo) is reported inside the bars of the histogram. The lines report the intragroup statistical analysis (T60 vs T0). Statistical analysis is marked with (*) in increasing numbers depending on significance.

References

    1. Food and Nutrition Board, Institute of Medicine. Dietary Reference Intakes. Washington, DC: National Academy Press; 2002.
    1. Steenvoorden DP, van Henegouwen GM. The use of endogenous antioxidants to improve photoprotection. J Photochem Photobiol. 1997;41:1–10.
    1. Thiele JJ, Dreher F, Packer L. Cosmeceuticals. In: Elsner P, Maibach H, editors. Drugs vs Cosmetics. New York, NY: Dekker; 2000.
    1. Sies H. Polyphenols and health: update and perspectives. Arch Biochem Biophys. 2010;501:2–5.
    1. Kiselev KV. Perspectives for production and application of resveratrol. Appl Microbiol Biotechnol. 2011;90:417–425.
    1. Agarwal B, Bauer JA. Resveratrol and life extension. Ann N Y Acad Sci. 2011;1215:138–143.
    1. Chen D, Wan SB, Yang H, et al. EGCG green tea polyphenol and their synthetic analogs for human cancer prevention and treatment. Adv Clin Chem. 2011;53:155–177.
    1. Wang Y, Ho CT. Polyphenolic chemistry of tea and coffee: a century of progress. J Agric Food Chem. 2009;57:8109–8114.
    1. Zhou H, Beevers CS, Huang S. The targets of curcumin. Curr Drug Targets. 2011;12:332–347.
    1. Katiyar SK, Agarwal R, Mukhtar H. Inhibition of tumor promotion in SENCAR mouse skin by ethanol extract of Zingiber officinale rhizome. Cancer Res. 1996;56:1023–1030.
    1. Surh Y. Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances. Mutat Res. 1999;428:305–327.
    1. McKenzie RC. Selenium, ultraviolet radiation and the skin. Clin Exp Dermatol. 2000;25:631–636.
    1. Stahl W, Sies H. Carotenoids and protection against solar UV radiation. Skin Pharmacol Appl Skin Physiol. 2002;15:291–296.
    1. Cesarini JP, Michel L, Maurette JM, et al. Immediate effects of UV radiation on the skin: modification by an antioxidant complex containing carotenoids. Photodermatol Photoimmunol Photomed. 2003;19:182–189.
    1. Guo W, Kong E, Meydani M. Dietary polyphenol, inflammation and cancer. Nutr Cancer. 2009;61:807–810.
    1. Jagdeo J, Adams L, Lev Tov H, et al. Dose-dependent antioxidant function of resveratrol demonstrated via modulation of reactive oxygen species in normal human skin fibroblast in vitro. J Drugs Dermatol. 2010;9:1523–1526.
    1. Bastianetto S, Dumont Y, Duranton A, et al. Protective action of resveratrol in human skin: possible involvement of specific receptor binding sites. PLoS One. 2010;5:e12935.
    1. Baxter RA. Anti-aging properties of resveratrol: review and report of a potent new antioxidant skin care formulation. J Cosmet Dermatol. 2008;7:2–7.
    1. Wie H, Saladi R, Lu Y, et al. Isoflavone genistein: photoprotection and clinical implications in dermatology. J Nutr. 2003;133(11 Suppl 1):S3811–S3819.
    1. Kenny TP, Keen CL, Jones P, et al. Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H2O2. Exp Biol Med. 2004;229:765–771.
    1. Cesarone MR, Belcaro G, Carratelli M, et al. A simple test to monitor oxidative stress. Int Angiol. 1999;18:127–130.
    1. Vassalle C, Masini S, Carpeggiani C, et al. In vivo total antioxidant capacity: comparison of two different analytical methods. Clin Chem Lab Med. 2004;42:84–89.
    1. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 1996;239:70–76.
    1. Courage W. Hardware and measuring principle: corneometer. In: Elsner P, Berardesca E, Maibach HI, editors. Bioengineering of the Skin: Water and the Stratum Corneum. London, UK: CRC Press; 1994.
    1. Berardesca E. EEMCO guidance for the assessment of stratum corneum hydration: electrical methods. Skin Research and Technology. 1997;3:126–132.
    1. Barel AO, Courage W, Clarys P. Suction method for measurement of skin mechanical properties: the cutometer. In: Serup J, Jemec GBE, editors. Handbook of Non-Invasive Methods and the Skin. Boca Raton, FL: CRC Press; 1995.
    1. Fujimura T, Haketa K, Hotta M, et al. Global and systematic demonstration for the practical usage of a direct in vivo measurement system to evaluate wrinkles. Int J Cosmet Sci. 2007;29:423–436.
    1. Hoppe U, Lunderstädt R, Sauermann G. Quantitative analysis of the skin’s surface by digital processing. In: Frosch PJ, Kligman AM, editors. Noninvasive Methods for the Quantification of Skin Functions. Heidelberg, Germany: Springer; 1993.
    1. Wilhelm KP, Elsner P, Berardesca E, Maibach HI. Bioengineering of the Skin: Skin Surface Imaging and Analysis. Boca Raton, FL: CRC Press; 1996.
    1. COLIPA Guidelines. Guideline for Colometric Determination of Skin Colour Typing and Prediction of the Minimal Erythemal Dose (MED) Without UV Exposure. European Cosmetic, Toiletry and Perfumery Association; 2007.
    1. Aggarwal BB. Targeting inflammation-induced obesity and metabolic diseases by curcumin and other nutraceuticals. Annu Rev Nutr. 2010;30:173–199.
    1. Prasad S, Phromnoi K, Yadav VR, et al. Targeting inflammatory pathways by flavonoids for prevention and treatment of cancer. Planta Med. 2010;76:1044–1063.
    1. Anand P, Kunnumakara A, Sundaram C, et al. Cancer is a preventable disease that requires major lifestyle changes. Pharm Res. 2008;25:2097–2116.
    1. Richelle M, Sabatier M, Steiling H, et al. Skin bioavailability of dietary vitamin E, carotenoids, polyphenols, vitamin C, zinc and selenium. Br J Nutr. 2006;96:227–238.
    1. Baur JA, Sinclair DA. Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov. 2006;5:493–506.
    1. Lagouge M, Argmann C, Gerhart-Hines Z, et al. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell. 2006;127:1109–1122.
    1. Zang M, Xu S, Maitland-Toolan KA, et al. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes. 2006;55:2180–2191.
    1. Halliwell B. Dietary polyphenols: good, bad, or indifferent for your health? Cardiovasc Res. 2007;73:341–347.
    1. Smoliga JM, Baur JA, Hausenblas HA. Resveratrol and health: a comprehensive review of human clinical trials. Mol Nutr Food Res. 2011;55:1–13.
    1. Chachay VS, Kirkpatrick CMJ, Hickman IJ, et al. Resveratrol – pills to replace a healthy diet? Br J Clin Pharmacol. 2011;72:27–38.
    1. Fabbrocini G, Staibano S, De Rosa G, et al. Resveratrol-containing gel for the treatment of acne vulgaris: a single-blind vehicle-controlled, pilot study. Am J Clin Dermatol. 2011;12:133–141.
    1. Morris B. Calorie restriction mimetics and aging. In: Everitt AV, Rattan SIS, Couteur DG, de Cabo R, editors. Calorie Restriction, Aging Longevity. Dordrecht, The Netherlands: Springer Publishing; 2010.
    1. Giacomoni PU, Declercq L, Hellemans L, et al. Aging of human skin: review of a mechanistic model and first experimental data. IUBMB Life. 2000;49:259–263.
    1. Hellemans L, Corstjens H, Neven A, et al. Antioxidant enzyme activity in human stratum corneum shows seasonal variation with an age-dependent recovery. J Invest Dermatol. 2003;120:434–439.
    1. Pillai S, Oresajo C, Hayward J. Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation. Int J Cosmet Sci. 2005;27:17–34.
    1. Bae JY, Choi J-S, Kang S-W, et al. Dietary compound ellagic acid alleviates skin wrinkle and inflammation induced by UV-B irradiation. Exp Dermatol. 2010;19:e182–e190.

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