Zika Virus in Rectal Swab Samples

Camila Helena Aguiar Bôtto-Menezes, Armando Menezes Neto, Guilherme Amaral Calvet, Edna Oliveira Kara, Marcus Vinícius Guimarães Lacerda, Marcia da Costa Castilho, Ute Ströher, Carlos Alexandre Antunes de Brito, Kayvon Modjarrad, Nathalie Broutet, Patrícia Brasil, Ana Maria Bispo de Filippis, Rafael Freitas Oliveira Franca, ZIKABRA Study Team, André Luiz de Abreu, Adele Schwartz Benzaken, Ximena Pamela Diaz Bermudez, Camila Helena Aguiar Bôtto-Menezes, Patrícia Brasil, Carlos Alexandre Antunes Brito, Nathalie Jeanne Nicole Broutet, Guilherme Amaral Calvet, Marcia da Costa Castilho, Ana Maria Bispo de Filippis, Rafael Freitas Oliveira Franca, Silvana Pereira Giozza, Ndema Habib, Edna Oliveira Kara, Marcus Vinicius Guimarães Lacerda, Sihem Landoulsi, Morganna Costa Lima, Noemia Lima, Maeve Brito de Mello, Ana Izabel Costa de Menezes, Robyn Meurant, Kayvon Modjarrad, Armando Menezes Neto, Cristina Pimenta, Casey Storme, Ute Ströher, Anna Thorson, Lydie Trautman, Camila Helena Aguiar Bôtto-Menezes, Armando Menezes Neto, Guilherme Amaral Calvet, Edna Oliveira Kara, Marcus Vinícius Guimarães Lacerda, Marcia da Costa Castilho, Ute Ströher, Carlos Alexandre Antunes de Brito, Kayvon Modjarrad, Nathalie Broutet, Patrícia Brasil, Ana Maria Bispo de Filippis, Rafael Freitas Oliveira Franca, ZIKABRA Study Team, André Luiz de Abreu, Adele Schwartz Benzaken, Ximena Pamela Diaz Bermudez, Camila Helena Aguiar Bôtto-Menezes, Patrícia Brasil, Carlos Alexandre Antunes Brito, Nathalie Jeanne Nicole Broutet, Guilherme Amaral Calvet, Marcia da Costa Castilho, Ana Maria Bispo de Filippis, Rafael Freitas Oliveira Franca, Silvana Pereira Giozza, Ndema Habib, Edna Oliveira Kara, Marcus Vinicius Guimarães Lacerda, Sihem Landoulsi, Morganna Costa Lima, Noemia Lima, Maeve Brito de Mello, Ana Izabel Costa de Menezes, Robyn Meurant, Kayvon Modjarrad, Armando Menezes Neto, Cristina Pimenta, Casey Storme, Ute Ströher, Anna Thorson, Lydie Trautman

Abstract

We detected Zika virus RNA in rectal swab samples from 10 patients by using real-time reverse transcription PCR, and we isolated the virus from 1 patient. The longest interval from symptom onset to detection was 14 days. These findings are applicable to diagnosis and infection prevention recommendations.

Keywords: Brazil; Zika virus; infection; rectal swab; sexual transmission; virus persistence; viruses.

Figures

Figure
Figure
Detection of Zika virus RNA in human biological specimens from 8 patients, according to Ct and days after disease onset. Patient identification numbers above charts correspond to numbers in the Table. Horizontal dashed lines indicate real-time reverse transcription PCR cutoff Ct of 38. Disease onset is day 0 (screening visit), defined after interviewing patients about symptoms. Ct, cycle threshold.

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Source: PubMed

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