The Influence of Probiotic Lactobacillus casei in Combination with Prebiotic Inulin on the Antioxidant Capacity of Human Plasma

Paulina Kleniewska, Arkadiusz Hoffmann, Ewa Pniewska, Rafał Pawliczak, Paulina Kleniewska, Arkadiusz Hoffmann, Ewa Pniewska, Rafał Pawliczak

Abstract

The aim of the present study was to assess whether probiotic bacteria Lactobacillus casei (4 × 10(8) CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n = 32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containing L. casei plus Inulin resulted in a significant increase in FRAP values (p = 0.00008) and CAT activity (p = 0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containing L. casei (4 × 10(8) CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes.

Figures

Figure 1
Figure 1
The influence of combination of probiotic Lactobacillus casei (4 × 108 CFU) and prebiotic Inulin (400 mg) on the FRAP values. Data is shown as mean ± SEM. #p = 0.00008; &p = 0.008; ∧p = 0.004 versus control group.
Figure 2
Figure 2
The influence of combination of probiotic Lactobacillus casei (4 × 108 CFU) and prebiotic Inulin (400 mg) on the CAT activity. Data is shown as mean ± SEM. ∗p = 0.02; ∗∗p = 0.007 versus control group.
Figure 3
Figure 3
The influence of combination of probiotic Lactobacillus casei (4 × 108 CFU) and prebiotic Inulin (400 mg) on the SOD activity. Data is shown as mean ± SEM.

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